Characterization and expression pattern analysis of the facilitative glucose transporter 10 gene (slc2a10) in Danio rerio

Abstract

The SLC2A10 gene located on chromosome 20q13.1 encodes the facilitative glucose transporter 10 (GLUT10), a class III member of the SLC2A facilitative glucose transporter family. Mutations in the human SLC2A10 gene cause arterial tortuosity syndrome (ATS), a rare autosomal recessive connective tissue disorder. In this work, we report the characterization of the slc2a10 ortholog gene in zebrafish (Danio rerio) and its expression pattern during embryonic development and in adult tissues. The slc2a10 gene consists of 5 exons, spanning 8 kb and mapping to a region on chromosome 11 that exhibits conserved synteny with human chromosome 20. The gene encodes Glut10, a 513 amino acid protein that maintains the 12 transmembrane domain structure typical of the GLUTs family, and shares the specific functional motifs involved in sugar transport with the vertebrate GLUT10. RT-PCR analysis showed that two specific splice variants, both including the 5'-UTR region, were expressed during embryogenesis and in different adult zebrafish tissues and organs. In situ hybridization analyses demonstrated a maternal origin of the total slc2a10 mRNA and its ubiquitous distribution until the early somitogenesis stage. In later embryonic stages, slc2a10 mRNA was detected in the otic vesicles, hatching gland cells, pectoral fin, posterior tectum and swim bladder. Overall, these results suggest a wide role of slc2a10 during zebrafish development.

GLUT10 deficiency leads to oxidative stress and non-canonical αvβ3 integrin-mediated TGFβ signalling associated with extracellular matrix disarray in arterial tortuosity syndrome skin fibroblasts

“…Mouse models failed to recapitulate the ATS vascular phenotype because vascular abnormalities were absent in mice with Slc2a10 homozygous missense substitutions ( 11 , 12 ). The zebrafish model better highlighted the essential role of GLUT10 in cardiovascular development because knockdown of the slc2a10 orthologue, which is broadly expressed during embryogenesis ( 13 ), was associated with several cardiovascular defects ( 14 ).…”

The pathobiology of vascular malformations: insights from human and model organism genetics

“…The cloning of SLC2A10 revealed structural similarities to other facilitative glucose transporters , indicating that glucose transport may be a main function of this protein. Expression of SLC2A10 has been identified in many tissues, with high expression noted in the pancreas, stomach, aorta, and adipose tissue . Furthermore, within the aorta, VSMCs have been shown to express high levels of SLC2A10 .…”

Transcript Analysis of Zebrafish GLUT3 Genes, slc2a3a and slc2a3b, Define Overlapping as Well as Distinct Expression Domains in the Zebrafish (Danio rerio) Central Nervous System

“…Recently, models for symptom dimensions of psychiatric disorders such as autism and schizophrenia, as well as for the neurodegenerative diseases Alzheimer’s and Parkinson’s diseases have been developed (Stewart et al, 2015; Fontana et al, 2018). Only some members of the GLUT family of proteins have been investigated in zebrafish to date and current knowledge is restricted to GLUT1, 2, 3, 10 and 12 (Tseng et al, 2009; Zheng et al, 2010; Chiarelli et al, 2011; Willaert et al, 2012; Carayannopoulos et al, 2014; Jiménez-Amilburu et al, 2015; Marín-Juez et al, 2015; Kuwabara et al, 2018). One zebrafish gene orthologous to human SLC2A3 has been identified ( slc2a3a , previously termed glut3 ; Tseng et al, 2009) and its expression pattern briefly described (Carayannopoulos et al, 2014).…”