Molecular Cloning, Identification, and Expression Patterns of Myostatin Gene in Water Buffalo (<i>Bubalus Bubalis</i>)

Zhu, Peng; Li, Haiyang; Huang, Guiting; Cui, Jiayu; Zhang, Ruimen; Cui, Kuiqing; Yang, Sufang; Shi, Dongquan

doi:10.1080/10495398.2017.1289941

Cited by 7 publications

(5 citation statements)

References 29 publications

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“…The Inhibin Subunit Beta A ( INHBA ) gene was characterized in buffalo bulls and single amino acid variations detected at cleaving sites with potential association with growth, maintenance, and reproduction [ 15 ]. Moreover, myostatin ( MSTN) is involved in skeletal muscle growth, and additionally was also found to be involved in folliculogenesis [ 16 ]. Water buffalo is an economically important genetic asset that contributes more than 15% of the world’s total milk supply [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of TGF-Beta Gene Family in Buffalo to Identify Gene Duplication and Functional Mutations

Saif-ur-Rehman

Hassan

Rehman

et al. 2022

Genes

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The TGF-β superfamily is ubiquitously distributed from invertebrates to vertebrates with diverse cellular functioning such as cell adhesion, motility, proliferation, apoptosis, and differentiation. The present study aimed to characterize the TGF-β gene superfamily in buffalo through evolutionary, structural, and single nucleotide polymorphism (SNPs) analyses to find the functional effect of SNPs in selected genes. We detected 32 TGF-β genes in buffalo genome and all TGF-β proteins exhibited basic nature except INHA, INHBC, MSTN, BMP10, and GDF2, which showed acidic properties. According to aliphatic index, TGF-β proteins were thermostable but unstable in nature. Except for GDF1 and AMH, TGF-β proteins depicted hydrophilic nature. Moreover, all the detected buffalo TGF-β genes showed evolutionary conserved nature. We also identified eight segmental and one tandem duplication event TGF-β gene family in buffalo, and the ratio of Ka/Ks demonstrated that all the duplicated gene pairs were under selective pressure. Comparative amino acid analysis demonstrated higher variation in buffalo TGF-β gene family, as a total of 160 amino acid variations in all the buffalo TGF-β proteins were detected. Mutation analysis revealed that 13 mutations had an overall damaging effect that might have functional consequences on buffalo growth, folliculogenesis, or embryogenesis.

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Section: Introductionmentioning

confidence: 99%

Molecular Characterization of TGF-Beta Gene Family in Buffalo to Identify Gene Duplication and Functional Mutations

Saif-ur-Rehman

Hassan

Rehman

et al. 2022

Genes

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“…RNA isolation, cDNA synthesis, and real-time quantitative polymerase chain reaction Total RNA extraction, cDNA synthesis, and real-time quantitative polymerase chain reaction (qPCR) were performed as previously described (Zhu et al, 2018). The expression levels of the transcript mRNAs were calculated by the 2 -66ct method.…”

Section: Bovine Tissue Sample Preparationmentioning

confidence: 99%

“…The full-length sequence of the ORF (Open Reading Frame) region of SQLE ( Supplementary Table S1) was amplified using Q5 Ò Super fidelity 2 • Master Mix (DNA polymerase, NEW ENGLAND Biolabs Ò ). In addition, bioinformatics analysis of the ORF region of SQLE gene was carried out according to the method as previously described (Zhu et al, 2018). To explore the role of SQLE in bovine MSCs, SQLE overexpression vector was constructed.…”

Section: Gene Cloning and Vector Constructionmentioning

confidence: 99%

SQLEPromotes Differentiation and Apoptosis of Bovine Skeletal Muscle-Derived Mesenchymal Stem Cells

Zhang¹,

Deng²,

Lv³

et al. 2020

Cellular Reprogramming

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In this study, Squalene epoxidase (SQLE) overexpression vector was transfected into bovine skeletal musclederived mesenchymal stem/stromal cells (MSCs) to study the molecular mechanism of SQLE regulating meat quality through myogenesis. We initially profiled the expression of SQLE in cattle embryos and adults, in the muscle tissue of four different cattle varieties, and in 11 different tissues/organs of Guangxi cattle variety. Subsequently, we isolated and cultured bovine skeletal muscle-derived MSCs and detected the expression of SQLE during cell proliferation and differentiation. Then, we constructed a bovine SQLE overexpression vector and transfected it into bovine skeletal muscle-derived MSCs by liposome transfection. Cell Counting Kit-8 (CCK-8), 5-ethynyl-20-deoxyuridine (EdU), flow cytometry, immunofluorescence, and quantitative polymerase chain reaction assays were used to characterize cell proliferation and differentiation in detail. The results showed that the relative expression level of bovine SQLE gene in brain tissue was the highest, and in adult muscle tissue was significantly higher than that in embryonic stage. Especially, the expression of SQLE was significantly upregulated in cell differentiation stage. Furthermore, the proliferation, cell cycle, apoptosis, and myoblast differentiation assays indicated that SQLE significantly promoted the differentiation and apoptosis of bovine skeletal muscle-derived MSCs, but inhibited their proliferation. In conclusion, our study reveals the role of SQLE in myoblast differentiation. These results will provide new clues for the regulation network of bovine muscle development.

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“…Inhibition of MSTN and knockdown of the MSTN gene cause muscle hypertrophy, which results in ‘double-muscled' cattle, sheep, mice, rabbits, dogs, and humans [ 1 ]. MSTN is predominately expressed in skeletal muscles but is also expressed in other tissues [ 2 , 3 ]. As a signaling ligand, MSTN modulates muscle mass development through the MSTN-activin receptor IIB- (ActRIIB-) Smad2/3 signaling pathway [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Myostatin Deficiency Enhances Antioxidant Capacity of Bovine Muscle via the SMAD-AMPK-G6PD Pathway

Zhu

Wang

Wei

et al. 2022

Oxidative Medicine and Cellular Longevity

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During exercise, the body’s organs and skeletal muscles produce reactive oxygen species (ROS). Excessive ROS can destroy cellular lipids, sugars, proteins, and nucleotides and lead to cancer. The production of nicotinamide adenine dinucleotide phosphate (NADPH) by the pentose phosphate pathway (PPP) is an auxiliary process of the cellular antioxidant system that supplements the reducing power of glutathione (GSH) to eliminate ROS in the cell. Myostatin (MSTN) is mainly expressed in skeletal muscle and participates in the regulation of skeletal muscle growth and development. Loss of MSTN leads to muscular hypertrophy, and MSTN deficiency upregulates glycolysis. However, the effect of MSTN on the PPP has not been reported. This study investigated the effect of MSTN on muscle antioxidant capacity from a metabolic perspective. We found that reducing MSTN modulates AMP-activated protein kinase (AMPK), a key molecule in cellular energy metabolism that directly regulates glucose metabolism through phosphorylation. Downregulation of MSTN promotes tyrosine modification of glucose-6-phosphate-dehydrogenase (G6PD) by AMPK and is regulated by the Smad signaling pathway. The Smad2/3 complex acts as a transcription factor to inhibit the AMPK expression. These results suggest that reduced MSTN expression inhibits the Smad signaling pathway, promotes AMPK expression, enhances the activity of G6PD enzyme, and enhances the antioxidant capacity of nonenzymatic GSH.

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Molecular Cloning, Identification, and Expression Patterns of Myostatin Gene in Water Buffalo (Bubalus Bubalis)

Abstract: MSTN, myostatin; ORF, open reading frame.

Cited by 7 publications

References 29 publications

Molecular Characterization of TGF-Beta Gene Family in Buffalo to Identify Gene Duplication and Functional Mutations

Molecular Characterization of TGF-Beta Gene Family in Buffalo to Identify Gene Duplication and Functional Mutations

SQLEPromotes Differentiation and Apoptosis of Bovine Skeletal Muscle-Derived Mesenchymal Stem Cells

Myostatin Deficiency Enhances Antioxidant Capacity of Bovine Muscle via the SMAD-AMPK-G6PD Pathway

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