Molecular cloning, functional characterization and phylogenetic analysis of B-cell activating factor in zebrafish (Danio rerio)

Molecular structure, expression and bioactivity characterization of TNF13B (BAFF) gene in mefugu, Takifugu obscurus

“…It is consistent with the previous studies such as Liang et al. [36] and Glenney et al [37]. The real-time quantitative PCR results showed that fBAFF gene was predominantly expressed in spleen as the same as rainbow trout [38].…”

Molecular cloning, expression, bioinformatics analysis and bioactivity characterization of TNF13B (BAFF) gene in bat (Vespertilio superans Thomas)

“…Studies have demonstrated that, due to the highly conserved nature of BAFF, functional cross-reactivity exists among different species, the BAFF of other species can bind to mouse BAFF receptors and has bioactivity on mouse B cells [27,28]. In this study, MTT assay verified that bsBAFF has similar bioactivity on mouse splenic B-cells as msBAFF (mouse BAFF).…”

The first BAFF gene cloned from the cartilaginous fish

“…Strangely, the BAFF gene of the stickleback (Gasterosteus aculeatus), has a furin protease cleavage site RKKR, but no such site was found in BAFFs from other bony fishes, such as the zebrafish (Danio rerio) or rainbow trout (Oncorhynchus mykiss) [23]. The absence of a signal peptide suggested that CpBAFF is a type II membrane protein, which is typical of the members of the TNF ligand family.…”

“…The sBAFF protein has been shown to be an anti-IgM co-stimulator of B-cell proliferation in vitro [45]. Studies have demonstrated that, due to the highly conserved nature of BAFF, functional cross-reactivity exists not only among mammalian and poultry BAFFs, but also between mammalian and fish BAFFs, such as zebrafish BAFF, which can bind to mouse BAFF receptors and has bioactivity on mouse Bcells [23,24,26]. In this study, a MTT assay verified that CpsBAFF has similar bioactivity on mouse splenic B-cells as hsBAFF.…”