Molecular cloning, expression and characterization of cDNAs encoding the ferritin subunits from the beetle, Apriona germari

Kim, Seong Ryul; Lee, Kwang-Sik; Yoon, Hyung Joo; Park, Nam Sook; Lee, Sang Mong; Kim, Iksoo; Seo, Sook Jae; Sohn, Hung Dae; Jin, Byung Rae

doi:10.1016/j.cbpc.2004.05.008

Cited by 19 publications

(15 citation statements)

References 32 publications

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“…Similarly ferritin is detected commonly throughout the life history of insects such as H. cunea (Kim et al, 2004b), D. melanogaster , Lind et al, 1998a, and Ae. aegypti (Geiser et al, 2003b.…”

Section: Discussionmentioning

confidence: 99%

“…aegypti mature LCH peptide (Asp22/23) (Geiser et al, 2003), known dipteran LCH homologues lack glycosylation, including G. m. morsitans (Lind et al, 1998a). Previously published alignments of insect LCHs, including that of D. melanogaster, maintain conservation of three Cys residues (Kim et al, 2004b, Kim et al, 2004a. In our alignment, GmmFer2LCH maintains the three conserved Cys residues in the N-terminus in alignment with D. melanogaster; however, in our alignment, the D. melanogaster sequence does not align with the conserved Cys residues of other insect sequences as was described previously (Kim et al, 2004b).…”

Section: Characterization Of G M Morsitans Gmmfer2lch and Gmmfer1hcmentioning

confidence: 99%

“…aegypti , Galleria mellonella (Kim et al, 2002) and the moth Hyphantria cunea (Kim et al, 2004b). The cDNA sequence of the Ae.…”

Section: Characterization Of G M Morsitans Gmmfer2lch and Gmmfer1hcmentioning

confidence: 99%

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Molecular characterization of iron binding proteins from Glossina morsitans morsitans (Diptera: Glossinidae)

Strickler-Dinglasan

Güz

Attardo

et al. 2006

Insect Biochemistry and Molecular Biology

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The regulation of iron is critical for maintaining homeostasis in the tsetse fly (Diptera:Glossinidae), in which both adult sexes are strict blood feeders. We have characterized the cDNAs for two putative iron-binding proteins (IBP) involved in transport and storage; transferrin (GmmTsf1) and ferritin from Glossina morsitans morsitans. GmmTsf1 transcripts are detected in the female fat body and in adult reproductive tissues, and only in the adult developmental stage in a bloodmeal independent manner. In contrast, the ferritin heavy chain (GmmFer1HCH) and light chain (GmmFer2LCH) transcripts are expressed ubiquitously, suggesting a more general role for these proteins in iron transport and storage. Protein domain predictions for each IBP suggest both the conservation and loss of several motifs present in their vertebrate homologues. In concert with many other described insect transferrins, putative secreted GmmTsf1 maintains 3 of the 5 residues necessary for ironbinding in the N-terminal lobe, but exhibits a loss of this iron-binding ability in the C-terminal role as well as a loss of large sequence blocks. Both putative GmmFer1HCH and GmmFer2LCH proteins have signal peptides, similar to other insect ferritins. GmmFer2LCH has lost the 5'UTR ironresponsive element (IRE) and, thus, translation is no longer regulated by cellular iron levels. On the other hand, GmmFer1HCH maintains both the conserved ferroxidase center and the 5'UTR IRE; however, transcript variants suggest a more extensive regulatory mechanism for this subunit.

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Section: Discussionmentioning

confidence: 99%

Section: Characterization Of G M Morsitans Gmmfer2lch and Gmmfer1hcmentioning

confidence: 99%

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Molecular characterization of iron binding proteins from Glossina morsitans morsitans (Diptera: Glossinidae)

Strickler-Dinglasan

Güz

Attardo

et al. 2006

Insect Biochemistry and Molecular Biology

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“…However, the L subunit has been found only in vertebrates [5,6]. The H subunit has seven conserved residues among different species which confer ferroxidase activity to the protein, converting Fe 2þ to Fe 3þ for a rapid detoxification of iron [7e10].…”

Section: Introductionmentioning

confidence: 99%

Single subunit type of ferritin from visceral mass of Saccostrea cucullata: Cloning, expression and cisplatin-subunit analysis

Zhu¹,

Lin²,

Cai³

et al. 2011

Fish & Shellfish Immunology

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“…In vertebrates, such complexes are composed of up to 24 ferritin heterodimers that comprise heavy ( H ) and light ( L ) subunits encoded by separate genes (Cairo et al, 1991;Orino et al, 1997). Although they are similar in structure among species from diff erent animal phyla in which the protein has been studied, the presence of L subunits appears to be restricted to ferritins from vertebrates (Theil, 2003;Kim et al, 2004). Interestingly, in these ferritins, cooperation of the H and L subunits is essential for effi cient iron uptake (Santambrogio et al, 1996).…”

Section: Introductionmentioning

confidence: 97%

Construction of a cDNA library for sea cucumber Acaudina leucoprocta and differential expression of ferritin peptide

Zhou

Hou²,

et al. 2016

Chin. J. Ocean. Limnol.

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Acaudina leucoprocta is an edible sea cucumber of economic interest that is widely distributed in China. Little information is available concerning the molecular genetics of this species although such knowledge would contribute to a better understanding of the optimal conditions for its aquaculture and its mechanisms of defense against disease. Therefore, we constructed a cDNA library and, based on bioinformatics analysis of the sequences, the functions of 75% of the cDNAs were identifi ed, including those involved in cell structure, energy metabolism, mitochondrial function, and signal transduction pathways. Approximately 25% of genes in the library were unmatched. The gene for A. leucoprocta ferritin was also cloned. The predicted amino-acid sequence of ferritin displayed signifi cant homology with other sea-cucumber counterparts but indicated that it was a new member of the ferritin family. Semiquantitative real-time RT-PCR indicated the highest levels of ferritin mRNA expression in the intestine. A polyclonal antibody of ferritin was also produced. These data provide a set of molecular tools essential for further studies of the functions of ferritin protein in A. leucoprocta .

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Molecular cloning, expression and characterization of cDNAs encoding the ferritin subunits from the beetle, Apriona germari

Cited by 19 publications

References 32 publications

Molecular characterization of iron binding proteins from Glossina morsitans morsitans (Diptera: Glossinidae)

Molecular characterization of iron binding proteins from Glossina morsitans morsitans (Diptera: Glossinidae)

Single subunit type of ferritin from visceral mass of Saccostrea cucullata: Cloning, expression and cisplatin-subunit analysis

Construction of a cDNA library for sea cucumber Acaudina leucoprocta and differential expression of ferritin peptide

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