Construction of a cDNA library for sea cucumber Acaudina leucoprocta and differential expression of ferritin peptide

Zhou, Jun; Hou, Fujing; Li, Ye; Su, Xiurong; Li, Taiwu; Jin, Chunhua

doi:10.1007/s00343-016-5010-8

Cited by 3 publications

(3 citation statements)

References 31 publications

(40 reference statements)

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“…The molecular mass of the purified product was in good agreement with the predicted molecular weight of ScFER, AjFER, and AlFER. These three protein bands had molecular weights between 20.1 and 29 kDa and were detected in the positive transformants in our previous work[ 36 – 38 ]. Here, recombinant ferritin from S .…”

Section: Resultsmentioning

confidence: 80%

“…japonicus , and A . Leucoprocta were expressed and purified as experimental materials, and the correctness was verified by western blot in the past[ 36 – 38 ]. The intensity of the recombinant protein bands increased with time, and peak expression of recombinant ferritin occurred 5 h after IPTG was introduced.…”

Section: Resultsmentioning

confidence: 99%

“…japonicus , and A . leucoprocta [ 36 – 38 ]. These three benthic animals are widely distribution in coastal areas of China.…”

Section: Discussionmentioning

confidence: 99%

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Preparation and Representation of Recombinant Mn-Ferritin Flower-Like Spherical Aggregates from Marine Invertebrates

et al. 2015

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Ferritin has important functions in the transition and storage of toxic metal ions, but its regulation and function in many invertebrate species are still largely unknown. In our previous work, the cDNA sequence of Sinonovacula constricta, Apostichopus japonicas and Acaudina leucoprocta were constructed and efficiently expressed in E. Coli BL21 under IPTG induction. In this follow-up study, the recombinant ferritins were exposed to heavy metal manganese. The manganese concentration levels in three recombinant ferritins were greater than horse spleen ferritin (HSF). Compared with HSF, the amount of manganese enrichment in the three recombinant ferritins was 1.75-fold, 3.25-fold and 2.42-fold increases in ScFER, AjFER, and AlFER, respectively. After phosphate stimulation, the concentration of manganese increased and was higher than the ordinary dialysis control groups. The ScFER was four times its baseline value. The AjFER and AlFER were 1.4- and 8-fold higher, respectively. The AlFER sample stimulated by phosphate was 22-fold that of HSF. The morphologies of the resulting Mn-Ferritin from different marine invertebrates were characterized with scanning electron microscopy. Surface morphologies were lamella flower-like and are consistent with changes in surface morphologies of the standard Mn-HSF. Invertebrate recombinant ferritin and HSF both can uptake manganese. We found that the structure of A. leucoproctarecombinant Mn-Ferritin aggregate changed over time. The surface formed lamella flower-like aggregate, but gradually merged to create a relatively uniform plate-like phase of aggregate spherically and fused without clear boundaries.

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Section: Resultsmentioning

confidence: 80%

Section: Resultsmentioning

confidence: 99%

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Preparation and Representation of Recombinant Mn-Ferritin Flower-Like Spherical Aggregates from Marine Invertebrates

et al. 2015

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Protective effect of polysaccharides of sea cucumber Acaudina leucoprocta on hydrogen peroxide-induced oxidative injury in RAW264.7 cells

Zhou

Xiang

et al. 2019

International Journal of Biological Macromolecules

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Conservative and Atypical Ferritins of Sponges

Adameyko

Burakov

Finoshin

et al. 2021

IJMS

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Ferritins comprise a conservative family of proteins found in all species and play an essential role in resistance to redox stress, immune response, and cell differentiation. Sponges (Porifera) are the oldest Metazoa that show unique plasticity and regenerative potential. Here, we characterize the ferritins of two cold-water sponges using proteomics, spectral microscopy, and bioinformatic analysis. The recently duplicated conservative HdF1a/b and atypical HdF2 genes were found in the Halisarca dujardini genome. Multiple related transcripts of HpF1 were identified in the Halichondria panicea transcriptome. Expression of HdF1a/b was much higher than that of HdF2 in all annual seasons and regulated differently during the sponge dissociation/reaggregation. The presence of the MRE and HRE motifs in the HdF1 and HdF2 promotor regions and the IRE motif in mRNAs of HdF1 and HpF indicates that sponge ferritins expression depends on the cellular iron and oxygen levels. The gel electrophoresis combined with specific staining and mass spectrometry confirmed the presence of ferric ions and ferritins in multi-subunit complexes. The 3D modeling predicts the iron-binding capacity of HdF1 and HpF1 at the ferroxidase center and the absence of iron-binding in atypical HdF2. Interestingly, atypical ferritins lacking iron-binding capacity were found in genomes of many invertebrate species. Their function deserves further research.

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Construction of a cDNA library for sea cucumber Acaudina leucoprocta and differential expression of ferritin peptide

Cited by 3 publications

References 31 publications

Preparation and Representation of Recombinant Mn-Ferritin Flower-Like Spherical Aggregates from Marine Invertebrates

Preparation and Representation of Recombinant Mn-Ferritin Flower-Like Spherical Aggregates from Marine Invertebrates

Protective effect of polysaccharides of sea cucumber Acaudina leucoprocta on hydrogen peroxide-induced oxidative injury in RAW264.7 cells

Conservative and Atypical Ferritins of Sponges

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