Molecular Cloning, Characterization, and mRNA Expression of Hemocyanin Subunit in Oriental River PrawnMacrobrachium nipponense

Abstract

Hemocyanin is a copper-containing protein with immune function against disease. In this study, a hemocyanin subunit named MnHc-1 was cloned from Macrobrachium nipponense. The full-length cDNA of MnHc-1 was 2,163 bp with a 2,028-bp open reading frame (ORF) encoding a polypeptide of 675 amino acids. The MnHc-1 mRNA was expressed in the hepatopancreas, gill, hemocytes, intestine, ovary, and stomach, with the highest level in the hepatopancreas. In the infection trial, the MnHc-1 mRNA transcripts in the hemocytes were significantly downregulated at 3 h after injection of Aeromonas hydrophila and then upregulated at 6 h and 12 h, followed by a gradual recovery from 24 to 48 h. The MnHc-1 transcriptional expression in the hepatopancreas was measured after M. nipponense were fed seven diets with 2.8, 12.2, 20.9, 29.8, 43.1, 78.9, and 157.1 mg Cu kg−1 for 8 weeks, respectively. The level of MnHc-1 mRNA was significantly higher in the prawns fed 43.1–157.1 mg Cu kg−1 diet than in that fed 2.8–29.8 mg Cu kg−1 diet. This study indicated that the MnHc-1 expression can be affected by dietary copper and the hemocyanin may potentially participate in the antibacterial defense of M. nipponense.

OTUs and ASVs Produce Comparable Taxonomic and Diversity from Shrimp Microbiota 16S Profiles Using Tailored Abundance Filters

“…In this regard, studies in different shrimp species have reported a higher diversity in the hepatopancreas than in intestine samples, both of which are part of the gastrointestinal tract [ 12 , 45 ]. This may reflect an increased organ-specific selecting pressure, possibly due to its role in the immune response through the production of lectin, hemocyanin, ferritin, antibacterial proteins, proteolytic enzymes, and nitric oxide [ 46 , 47 , 48 , 49 ].…”

Trypsin polymorphism and modulation in Penaeus vannamei (Boone, 1931): a review

“…As the DNA sequences from family III were not found in the cDNA from the DG of P. vannamei under laboratory conditions (Klein et al, 1996), several questions remain open such as: why were the corresponding cDNAs not detected by screening? Could it be because of the low expression in the DG or because this gene is expressed in tissues other than the DG as hemocyanin in Macrobrachium nipponense, which is mainly expressed in the digestive gland, but not in the muscle (Kong et al, 2016). Although the gene is not found in adult P. vannamei, it may be present in other life stages as reported for the enzyme α-amylase, which occurs in the same P. vannamei, with an increase of α-amylase isoforms in adults (from 7 to 10 isoforms) revealed by SDS-PAGE (Castro et al, 2012).…”

Molecular Cloning, Structure and Phylogenetic Analysis of a Hemocyanin Subunit from the Black Sea Crustacean Eriphia verrucosa (Crustacea, Malacostraca)

“…With the progress in biotechnology and the development of next-generation sequencing technologies in the last decade, the studies on these important proteins made enormous progress in their isolation, structural, and functional characterization. Until now, the primary structure of hemocyanins have been described in several organisms belonging to suborders: Dendrobranchiata ( L. vannamei , L. monodon , M. japonicas , F. chinensis , F. merguiensis , M. magister , A. vulgare ) and Pleocyemata ( E. sinensis , A. moluccensis , C. sapidus , C. aestuarii , C. multidentata , C. quadricarinatus , P. carinicauda , P. interruptus , P. vulgaris , P. elephas , M. nipponense , M. rosenbergii , S. paramamosain , S. serrata , H. americanus , P. clarkii ) of order Decapoda; suborders Senticaudata ( G. roeselii ) and Corophiida ( C. scammon ) of order Amphypoda and suborders Limnoriidea ( L. quadripunctata ), Cymothoida ( E. pulchra ), Oniscidea ( P. scaber ), and Peracarida ( A. vulgare ) of order Isopoda, all belonging to subphylum Crustacea [ 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ].…”

“…Of the α- and γ-types, seven distinct variants have been described in crustaceans [ 21 ]. Until now, hemocyanins have been isolated from several organisms belonging to orders Decapoda, Amphipoda, and Isopoda of class Malacostraca [ 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ].…”