Molecular Cloning, Characterization, and mRNA Expression of Intestinal Fatty Acid Binding Protein (<i>I-FABP</i>) in <i>Columba Livia</i>

Abstract

Intestinal fatty acid binding protein (I-FABP) is involved in fatty acid transportation in mammals. To verify its role in fatty acid metabolism in birds, the model of pigeon intestinal organ culture was established, and a full-length cDNA of I-FABP was cloned from the intestine of Columba livia by rapid amplification of cDNA ends (RACE) method for the first time. The full length cDNA of C. livia I-FABP was 855 bp, including a 5′ untranslated region (UTR) of 34 bp, a 3′ UTR of 422 bp and an open reading frame (ORF) of 399 bp encoding a protein of 132 amino acids with the predicted molecular weight of 15.13 kDa. Sequence comparison indicated that I-FABP of C. livia had high identity with other avian I-FABP and belonged to the first subfamily of FABPs. Using quantitative real-time PCR, pigeon I-FABP mRNA in duodenum and jejunum increased progressively with development stage. I-FABP mRNA was expressed at the highest level at 28 day post hatch in the duodenum, while in ileum, it reached the maximum at the day of hatch, and decreased subsequently. The effects of fatty acids on pigeon I-FABP expression were also investigated in vitro. The results showed that significant increases in the pigeon I-FABP mRNA level were induced by linoleic acid and arachidonic acid, whereas I-FABP gene expression appeared to be unaffected by oleic acid and α-linolenic acid. The results indicate that I-FABP may be indicative of intestine development in pigeon, and it could be regulated by n-6 polyunsaturated fatty acids.

Effects of incubation and chick rearing on intestinal morphology, digestive enzyme activities, and mRNA expression of nutrient transporter genes in the pigeon (Columba livia) under artificial farming conditions

“…Nutrient transporters can be representatives of intestine development in pigeons ( Dong et al., 2012b , Xie et al., 2012 , Xie et al., 2013 , Zhang et al., 2017 ). FAT/CD36 and fatty acid–binding protein ( FABP ) are important components of the long-chain fatty acid transport system in various types of cells ( Veerkamp and Maatman, 1995 , Febbraio et al., 2001 ).…”

Intestinal fatty acid binding protein gene (I-FABP) in golden pompano Trachinotus ovatus (Linnaeus 1758) larvae: ontogenetic expression and response to water temperature and nutrition manipulation

“…To the best of our knowledge, there was no report on the expression level of I-FABP gene during early development of fish larvae. In terrestrial species such as pigeon, chickens, and turkeys, the expression of I-FABP gene significantly increases after hatching (Ding and Lilburn, 2002;Katongole and March, 1980;Xie et al, 2013), while the expression level of I-FABP gene in mice rises rapidly on day 17 during embryonic development (Green et al, 1992). Furthermore, the increase of I-FABP over time after hatching may be correlated with the uptake of dietary fatty acids after the formation of a functional digestive tract at the late developmental stage of larval golden pompano.…”

Bioinformatics analysis and tissue-specific expression of intestinal-type fatty acid binding protein in domestic pigeons (Columba livia)

“…In our previous study, the full-length cDNA of FABP2 gene had been cloned from the intestine of domestic pigeons. The expression of FABP2 gene showed a distinct development pattern in the duodenum and ileum, which could be an indicator of intestinal development in pigeons (Xie et al 2013). The FABP2 gene has been studied mainly in mammals, but its physiological function remains to be clearly defined.…”