Molecular cloning, characterization and expression analysis of cathepsin D gene from turbot Scophthalmus maximus

Molecular characterization and expression analysis of the channel catfish cathepsin D genes

“…Cathepsin D was reported in other organisms to be constitutively expressed in almost all cell types, with much elevated transcript expression in certain forms of cancer and in maturing oocyte of egg-laying animals [21,32]. Consistent with the current study of catfish cathepsin D, cathepsin D genes from other teleost fish studied to-date were found to be expressed in a wide range of tissue types [6,12,13,15,17,18,20,22]. This is in agreement with the fact that the cathepsin D gene is a housekeeping proteinase in the lysosome.…”

“…Cathepsin D has been cloned and sequenced in a number of mammalian species [9,10], and in several species of fish such as tilapia (Tilapia nilotica  Tilapia aurea) [11], rainbow trout (Oncorhynchus mykiss) [12], Antarctic icefish (Chionodraco hamatus) [13], seabream (Sparus aurata) [14], zebrafish (Danio rerio) [15], carp (Cyprinus carpio) [16], herring (Clupea harengus) [17], pufferfish (Takifugu rubripes) [18], Atlantic cod (Gadus morhua) [19], and turbot (Scophthalmus maximus) [20]. In most fish species, cathepsin D was found to be mainly expressed in the spleen and liver [19], but it is also abundant in skin mucosa [21] and muscle based on protein levels [11,17,22].…”

Molecular characterization and expression analysis of Cathepsin B and L cysteine proteases from rock bream (Oplegnathus fasciatus)

“…Cathepsin expression is known to be induced by several stimulators, such as LPS, virus, poly I:C [29], bacteria Vibrio harveyi [30], tumor promoters [31] and growth factors [32]. As a part of the Gram-negative bacteria cell wall, the LPS endotoxin induced the expression of large number of genes related to inflammation, cytokine activity, antigen presentation and binding [33].…”

Identification and expression analysis on bactericidal permeability-increasing protein/lipopolysaccharide-binding protein of blunt snout bream, Megalobrama amblycephala

“…The immune system of fish is obviously different from that of mammals because of its lacking bone marrow and lymph nodes. In contrast, fish use usually the kidney as a major lymphoid organ [50]. Furthermore, fish possess a thymus, spleen, head kidney and mucosa-associated lymphoid tissues (MALT, i.e.…”