Identification and expression analysis on bactericidal permeability-increasing protein/lipopolysaccharide-binding protein of blunt snout bream, Megalobrama amblycephala

Tang, Leilei; Liang, Yinhua; Jiang, Yuhong; Liu, Shaojun; Zhang, Fuyun; He, Xia; Wang, Tian-Yi; Zhou, Yi; Zhong, Huan; Ji, Yan

doi:10.1016/j.fsi.2015.05.013

Cited by 24 publications

(11 citation statements)

References 50 publications

(45 reference statements)

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“…This antimicrobial effector selectively binds to LPS, determining the specificity of recognition for Gram-negative bacteria, and leads to bacterial cell lysis 75 . The conserved antimicrobial function of fish BPIs, demonstrated by multiple studies 76–78 , implies that its induction in salmon might provide increased protection against Gram-negative bacterial infection. This activity could be complementary to that of lysozyme, expected to confer increased protection against Gram-positive bacterial infections.…”

Section: Resultsmentioning

confidence: 99%

Dietary fatty acid source has little effect on the development of the immune system in the pyloric caeca of Atlantic salmon fry

Jalili

Jin

Bones

et al. 2019

Sci Rep

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The quality and relative amounts of dietary lipids may affect the health and growth of cultured Atlantic salmon. So far, little is known about their effects on the performance of the fish immune system during early life stages and, in particular their importance in the transition from endogenous nutrition (yolk) in the alevin stage to exogenous nutrition in the later fry stage. We investigated the immunomodulatory effects of fish oil, vegetable oil and phospholipid-rich oil in feeds for farmed Atlantic salmon using a transcriptomic approach. The experiment allowed a fine-scale monitoring of gene expression profiles in two tissues, the pyloric caeca of the intestine and the liver, in a 94 days-long first feeding experiment. The analysis of transcriptional profiles revealed that first feeding induced a strong immunomodulation in the pyloric caeca after 48 days of feeding, lasting up to day 94 and possibly beyond. On the other hand, the differential effect of the three dietary regimes was negligible. We interpret this upregulation, undetectable in liver, as a potentiation of the immune system upon the first contact of the digestive system with exogenous feed. This process involved a complex network of gene products involved in both cellular and humoral immunity. We identified the classical pathway of the complement system, acting at the crossroads between innate and adaptive immunity, as a key process modulated in response to the switch from endogenous to exogenous nutrition.

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Section: Resultsmentioning

confidence: 99%

Dietary fatty acid source has little effect on the development of the immune system in the pyloric caeca of Atlantic salmon fry

Jalili

Jin

Bones

et al. 2019

Sci Rep

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show abstract

“…The response of immune function related genes to LPS was tissue-specific, compared to the other tissues. Nevertheless, changes in the humoral response due to bacterial pathogen exposure was reported in Ictalurus punctatus [116] and Megalobrama amblycephala [117] and bpi, one of the modified gene transcripts, was also differentially expressed in N. coriiceps. Similarly, peptidoglycan recognition proteins (e.g., pgrp-lbl), that bind multiple components of bacteria, a well characterized part of the innate humoral response in Danio rerio [118] and Cyprinus carpio L. [119], were changed in N. coriiceps.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Down-Regulation of Immune System Components in Barrier and Hematopoietic Tissues after Lipopolysaccharide Injection in Antarctic Notothenia coriiceps

Sousa

Power

Guerreiro

et al. 2022

Fishes

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The environmental conditions and isolation in the Antarctic have driven the evolution of a unique biodiversity at a macro to microorganism scale. Here, we investigated the possible adaptation of the teleost Notothenia coriiceps immune system to the cold environment and unique microbial community of the Southern Ocean. The fish immune system was stimulated through an intraperitoneal injection of lipopolysaccharide (LPS 0111:B4 from E. coli) and the tissue transcriptomic response and plasma biochemistry were analyzed 7 days later and compared to a sham injected control. Gene transcription in the head-kidney, intestine and skin was significantly modified by LPS, although tissues showed different responsiveness, with the duodenum most modified and the skin the least modified. The most modified processes in head-kidney, duodenum and skin were related to cell metabolism (up-regulated) and the immune system (comprising 30% of differentially expressed genes). The immune processes identified were mostly down-regulated, particularly interleukins and pattern recognition receptors (PRRs), nucleotide-binding oligomerization domain-like receptors and mannose receptors, unlike the toll-like receptors response commonly described in other teleost fish. The modified transcriptional response was not mirrored by a modified systemic response, as the circulating levels of enzymes of innate immunity, lysozyme and antiproteases, were not significantly different from the untreated and sham control fish. In conclusion, while the N. coriiceps immune system shares many features with other teleosts there are also some specificities. Further studies should better characterize the PRRs and their role in Antarctic teleosts, as well as the importance of the LPS source and its consequences for immune activation in teleosts.

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“…5). Both V. alginolyticus and V. parahaemolyticus are gram-negative bacteria which are responsible for approximately one-third of aquaculture disease cases [28]. LPSs are a major component of the outer membrane of the gram-negative bacteria and can initiate potent innate immune responses that prime the immune system against further infection [29].…”

Section: Discussionmentioning

confidence: 99%

A novel innexin2 forming membrane hemichannel exhibits immune responses and cell apoptosis in Scylla paramamosain

Wang

Chen

Dong

et al. 2015

Fish & Shellfish Immunology

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Identification and expression analysis on bactericidal permeability-increasing protein/lipopolysaccharide-binding protein of blunt snout bream, Megalobrama amblycephala

Cited by 24 publications

References 50 publications

Dietary fatty acid source has little effect on the development of the immune system in the pyloric caeca of Atlantic salmon fry

Dietary fatty acid source has little effect on the development of the immune system in the pyloric caeca of Atlantic salmon fry

Transcriptomic Down-Regulation of Immune System Components in Barrier and Hematopoietic Tissues after Lipopolysaccharide Injection in Antarctic Notothenia coriiceps

A novel innexin2 forming membrane hemichannel exhibits immune responses and cell apoptosis in Scylla paramamosain

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