Molecular cloning, characterization and expression analysis of peroxiredoxin 6 from disk abalone Haliotis discus discus and the antioxidant activity of its recombinant protein

Nikapitiya, Chamilani; Zoysa, Mahanama De; Whang, Ilson; Kim, Choon-Gon; Lee, Youn-Ho; Kim, Sang-Jin; Lee, Jehee

doi:10.1016/j.fsi.2009.05.002

Cited by 38 publications

(16 citation statements)

References 51 publications

(77 reference statements)

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“…In vitro, Ile inhibited lipid peroxidation of ox-brain phospholipids [68]. The lipid peroxidation and protein oxidation may result from generation of excess ROS [69], which can be removed by non-enzymatic antioxidants such as GSH and antioxidant enzymes such as SOD, CAT, GPx and GR in fish [15]. Therefore, in the current study, non-enzymatic and enzymatic antioxidant responses were also measured.…”

Section: Ile Improved the Intestinal Antioxidant Status Of Fishmentioning

confidence: 88%

Effect of dietary isoleucine on the immunity, antioxidant status, tight junctions and microflora in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian)

Zhao

Feng

Liu

et al. 2014

Fish & Shellfish Immunology

129

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Section: Ile Improved the Intestinal Antioxidant Status Of Fishmentioning

confidence: 88%

Effect of dietary isoleucine on the immunity, antioxidant status, tight junctions and microflora in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian)

Zhao

Feng

Liu

et al. 2014

Fish & Shellfish Immunology

129

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“…cause DNA damage, lipid peroxidation). In molluscs, the Prx6 enzyme scavenges H 2 O 2 and provides protection against H 2 O 2 -induced cell apoptosis (Nikapitiya et al 2009). Down-regulation of the Prx6 gene in S. glomerata due to reduced salinity prior to infection with Marteilia sydneyi could possibly result in higher mortality of S. glomerata from H 2 O 2 -induced damage to host tissue.…”

Section: Discussionmentioning

confidence: 99%

Reduced salinity, but not estuarine acidification, is a cause of immune-suppression in the Sydney rock oyster Saccostrea glomerata

Green¹,

Barnes²

2010

Mar. Ecol. Prog. Ser.

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Acid sulfate soils (ASS) are a major problem around the world. Oxidation of these sulfide-rich sediments by anthropogenic disturbance results in production and release of sulfuric acid. Subsequent rise in the water table due to rain may result in sulfuric acid and heavy metal ions leaching out of the sediments into nearby estuaries, leading to their acidification. Anecdotal evidence exists that flood waters containing ASS leachate may cause adult Sydney rock oysters Saccostrea glomerata to become immune-suppressed, leading to higher mortality rates from the paramyxean parasite Marteilia sydneyi (aetiological agent of QX disease). In the current study, adult S. glomerata were exposed to a seawater matrix of varying salinity, sulfuric acid and Al 3+ for 48 h, and the expression of 7 genes involved in immunity were measured by quantitative reverse-transcription polymerase chain reaction. A change in salinity from 35 to 15 ppt caused a 1.7-fold down-regulation in the expression of peroxiredoxin 6 gene (p < 0.05). The expression of peroxiredoxin 6 was still down-regulated 5 d after oysters were returned to full-strength seawater (35 ppt). Changes in salinity, sulfuric acid and Al 3+ concentration had no effects on the expression of other target genes (p > 0.05). The effects of salinity, sulfuric acid and Al 3+ on the ability of immune genes to respond to microbial invasion were also investigated by exposing oysters to the seawater matrix for 40 h, followed by injection with heat-killed Vibrio alginolyticus bacteria. A drop in salinity coupled with V. alginolyticus injection resulted in down-regulation of peroxiredoxin 6 and C1q-like protein (p < 0.05). Apart from C1q-like protein, exposure to sulfuric acid and Al 3+ had no significant effects on the response of target genes to stimulation by V. alginolyticus (p > 0.05). These results support that reduced salinity, and not estuary acidification, is the main environmental stressor resulting in S. glomerata becoming immunocompromised during the QX disease risk period.KEY WORDS: Oyster · Saccostrea · Gene expression · Acidification · Environmental stress · Acid sulfate soils Resale or republication not permitted without written consent of the publisher

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“…Thioredoxin-related enzymes are involved in thiol-based redox regulation and belong to the thioredoxin (Trx) superfamily, which also consists of glutathione peroxidase, peroxiredoxin and glutaredoxin gene family members (Shchedrina et al, 2007). The activities of these enzymes have been proposed to protect against metal exposure and virus-induced oxidative stress in molluscs (Nikapitiya et al, 2009;Trevisan et al, 2011;, although none were significantly up-regulated in response to the treatments applied here. Contigs were also identified with high sequence similarity to superoxide dismutase (SOD) and catalase (CAT) enzymes.…”

Section: Detection Of Antioxidant Genes Of Interest For Ecotoxicologymentioning

confidence: 93%

Key metabolic pathways involved in xenobiotic biotransformation and stress responses revealed by transcriptomics of the mangrove oyster Crassostrea brasiliana

et al. 2015

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Key metabolic pathways involved in xenobiotic biotransformation and stress responses revealed by transcriptomics of the mangrove oyster Crassostrea brasiliana.Aquatic Toxicology http://dx.doi.org/10. 1016/j.aquatox.2015.06.012 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Highlights-Thetranscriptome of the mangrove oyster Crassostrea brasilianawas sequenced.-The sequencing effort dramatically expanded the existing cDNA sequences available for the species.-Global analysis for transcription in the oyster treated with phenanthrene, diesel and domestic sewage was performed.-The pollutants altered mRNAs for genes in biotransformation, antioxidant and stress response pathways. Abstract 2The Brazilian oyster Crassostrea brasiliana was challenged to three common environmental contaminants: phenanthrene, diesel fuel water-accommodated fraction (WAF) and domestic sewage. Total RNA was extracted from the gill and digestive gland, and cDNA libraries were sequenced using the 454 FLX pla orm. The assembled transcriptome resulted in ˜20,000contigs, which were annotated to produce the first de novo transcriptome for C. brasiliana.Sequences were screened to identify genes potentially involved in the biotransformation of xenobiotics and associated antioxidant defence mechanisms. These gene families included those of the cytochrome P450 (CYP450), 70kDa heat shock, antioxidants, such as glutathione Stransferase, superoxide dismutase, catalase and also multi-drug resistance proteins. Analysis showed that the massive expansion of the CYP450 and HSP70 family due to gene duplication identified in the Crassostrea gigas genome also occurred in C. brasiliana, suggesting these processes form the base of the Crassostrea lineage. Preliminary expression analyses revealed several candidates biomarker genes that were up-regulated during each of the three treatments, suggesting the potential for environmental monitoring.

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Molecular cloning, characterization and expression analysis of peroxiredoxin 6 from disk abalone Haliotis discus discus and the antioxidant activity of its recombinant protein

Cited by 38 publications

References 51 publications

Effect of dietary isoleucine on the immunity, antioxidant status, tight junctions and microflora in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian)

Effect of dietary isoleucine on the immunity, antioxidant status, tight junctions and microflora in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian)

Reduced salinity, but not estuarine acidification, is a cause of immune-suppression in the Sydney rock oyster Saccostrea glomerata

Key metabolic pathways involved in xenobiotic biotransformation and stress responses revealed by transcriptomics of the mangrove oyster Crassostrea brasiliana

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