Molecular Cloning and Single Nucleotide Polymorphism Detection of Buffalo DGAT1 Gene

Yuan, Jing; Zhou, Jun; Deng, Xuemei; Hu, Xiaoxiang; Li, Ning

doi:10.1007/s10528-007-9100-3

Cited by 18 publications

(27 citation statements)

References 22 publications

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“…In the current study, it was found that all four buffalo groups shared the same K genotype with K232A mutation, which was consistent with the report that K allele genotype associating with higher milk fat yield, higher fat and protein percentage (Tantia et al. 2006; Yuan et al. 2007).…”

Section: Discussionsupporting

confidence: 92%

DGAT1, GH, GHR, PRL and PRLR Polymorphism in Water Buffalo (Bubalus bubalis)

Shi¹,

Wang²,

Yang

et al. 2011

Reprod Domestic Animals

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The polymorphism of several genes has been shown to affect the milk composition traits in dairy cattle, including DGAT1-exon8 K232A, GH-intron3 MspI, GH-exon5 AluI, GHR-exon8 F279Y, PRL-exon3 RsaI and PRLR-exon3 S18N. However, the polymorphism and effects of these genes on the milk traits of water buffalo are still unclear. In this study, four DNA pooling samples from Murrah, Nili-ravi, Murrah-Nili-Swamp crossbreed and Chinese swamp buffalo were constructed, respectively, and polymorphism of these sites was investigated using PCR-Single-strand conformation polymorphism and sequencing. Twenty-eight inter-specific single-nucleotide polymorphism (SNPs) were found in these six assayed gene fragments between buffalo and dairy cattle, including nine intra-specific SNPs among buffalo groups. All buffalo fixed a K allele genotype in DGAT1-exon8, MspI(+) restriction site(c nucleotide) and AluI(+) site(c nucleotide) at intron3 and exon5 of GH gene, F allele genotype of F279Y mutation in GHR gene, RsaI(-) restriction site at PRL-exon3/exon4 and N allele genotype of S18N mutation at PRLR-exon3. It provides an indirect evidence that water buffalo have fixed alleles with genotypes reported in dairy cattle, which is thought to be responsible for high milk fat, high protein content and low milk yield. Moreover, three new intra-specific SNPs were found including 275th bp (c/t) in DGAT1 of Murrah buffalo, 109th bp (t/a) in PRL-exon3/exon4 and 43rd bp (c/t) in PRLR-exon3 of Chinese swamp buffalo. Information provided in this study will be useful in further studies to improve buffalo breeding for better lactation performances.

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Section: Discussionsupporting

confidence: 92%

DGAT1, GH, GHR, PRL and PRLR Polymorphism in Water Buffalo (Bubalus bubalis)

Shi¹,

Wang²,

Yang

et al. 2011

Reprod Domestic Animals

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“…Polymorphism was seen as a substitution at four positions (Table 1). Seven SNPs have been reported based on PCR-SSCP studies of the entire DGAT1 gene in Chinese buffalo (Yuan et al , 2007) and 19 SNPs (DQ886485) were reported based on sequencing studies in Indian buffalo (Mishra et al , 2007), but none have been reported for the region studied here. The sequence of allele A identified here was identical to that reported for the DGAT1 gene in Indian buffalo (DQ886485).…”

mentioning

confidence: 89%

Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP

Raut

Kumar²,

Kala

et al. 2012

Genet. Mol. Biol.

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Diacylglycerol O-acyltransferase 1 (DGAT1) is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. The DGAT1 gene is a strong functional candidate for determining milk fat content in cattle. In this work, we used PCR-SSCP (polymerase chain reaction-single-strand conformation polymorphism) and DNA sequencing to examine polymorphism in the region spanning exon 7 to exon 9 of the DGAT1 gene in Murrah and Pandharpuri buffaloes. Three alleles (A, B and C) and four novel single-nucleotide polymorphisms were identified in the buffalo DGAT1 gene. The frequencies of the alleles differed between the two buffalo breeds, with allele C being present in Murrah but not in Pandharpuri buffalo. The allele variation detected in this work may influence DGAT1 expression and function. The results described here could be useful in examining the association between the DGAT1 gene and milk traits in buffalo.

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“…They increased the fat content of the milk in Holstein cows (Spelman et al 2002) and decreased protein content and milk yield in Jersey cows (Weller et al 2003). Cardoso et al (2015) found that the a variable nucleotide repeat (VNRT) in the promoter region of DGAT1 that explains 32 % of additive genetic variance of fat percentage and Yuan et al (2007) found a nonsynonymous nucleotide substitution in DGAT1 gene (Ala484Val) in Murrah buffaloes not described in cattle, but without association studies.…”

Section: Introductionmentioning

confidence: 99%

Genetic association between SNPs in the DGAT1 gene and milk production traits in Murrah buffaloes

Freitas

Camargo

Stafuzza

et al. 2016

Trop Anim Health Prod

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This study identified polymorphisms in the DGAT1 gene in Murrah buffaloes and investigated the associations to milk production and quality traits (milk, fat and protein yields and percentages, somatic cell count). Genomic DNA was extracted from hair follicles collected from the tail of 196 females. Three SNPs were identified in DGAT1 gene by sequencing. Statistical analyses were performed to verify the linkage and the association between polymorphisms and traits. The estimated value of r (2) between two SNPs in exon 17 (g.11,783G > A and g.11,785 T > C) was 0.029. SNP g.11,785 T > C was significantly associated (P < 0.05) to fat and protein percentage. Dominance effect was significant for milk and fat yields and protein percentage (P < 0.05). The additive effect of the SNP g.11,785 T > C was significant for protein production and somatic cell count (P < 0.05). It indicates that assisted marker selection might be done with considerations to balance production and udder health.

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Molecular Cloning and Single Nucleotide Polymorphism Detection of Buffalo DGAT1 Gene

Cited by 18 publications

References 22 publications

DGAT1, GH, GHR, PRL and PRLR Polymorphism in Water Buffalo (Bubalus bubalis)

DGAT1, GH, GHR, PRL and PRLR Polymorphism in Water Buffalo (Bubalus bubalis)

Identification of novel single nucleotide polymorphisms in the DGAT1 gene of buffaloes by PCR-SSCP

Genetic association between SNPs in the DGAT1 gene and milk production traits in Murrah buffaloes

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