Molecular cloning and sequence variation of UDP-glucose pyrophosphorylase cDNAs from potatoes sensitive and resistant to cold sweetening

Sowokinos, Joseph R.; Вигдорович, В. И.; Abrahamsen, Mitchell S.

doi:10.1016/j.jplph.2004.04.006

Cited by 26 publications

(24 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Unlike most other enzymes involved in carbohydrate metabolism, UDPglucose pyrophosphorylase (spot 4713), known to be involved in sucrose biosynthesis or breakdown depending on the metabolic status of the tuber [23], did not show highly significant differences but increased slightly in mature tubers.…”

Section: Proteins Involved In Carbohydrate Metabolismmentioning

confidence: 79%

Proteomic analysis of the potato tuber life cycle

et al. 2006

View full text Add to dashboard Cite

The tuber of potato (Solanum tuberosum) is commonly used as a model for underground storage organs. In this study, changes in the proteome were followed from tuberization, through tuber development and storage into the sprouting phase. Data interrogation using principal component analysis was able to clearly discriminate between the various stages of the tuber life cycle. Moreover, five well-defined protein expression patterns were found by hierarchical clustering. Altogether 150 proteins showing highly significant differences in abundance between specific stages in the life cycle were highlighted; 59 of these were identified. In addition, 50 proteins with smaller changes in abundance were identified, including several novel proteins. Most noticeably, the development process was characterized by the accumulation of the major storage protein patatin isoforms and enzymes involved in disease and defense reactions. Furthermore, enzymes involved in carbohydrate and energy metabolism and protein processing were associated with development but decreased during tuber maturation. These results represent the first comprehensive picture of many proteins involved in the tuber development and physiology.

show abstract

Section: Proteins Involved In Carbohydrate Metabolismmentioning

confidence: 79%

Proteomic analysis of the potato tuber life cycle

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Snowden using RT-PCR (Sowokinos et al, 2004). The cDNA for the UgpA gene (1.5 kb XhoI/NcoI fragment) was fused into a pET21d bacterial expression vector without a polyhistidine tag.…”

Section: Cloning Of a Ugpase Cdna From Cultivar Snowdenmentioning

confidence: 99%

“…'Norchip' (UGP3) and the cold-sweetening resistant cv. 'Snowden' (UGP5) differed in their composition by only five amino acids (Sowokinos et al, 2004). Regardless, these five amino acid differences had a marked influence on substrate-binding efficiency and on other catalytic properties (Gupta and Sowokinos, 2003).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Regulation of UDP-glucose pyrophosphorylase isozyme UGP5 associated with cold-sweetening resistance in potatoes

Sowokinos

Hahn

2008

Journal of Plant Physiology

Self Cite

View full text Add to dashboard Cite

“…T405, Arabidopsis) and for binding of 14:3:3 proteins (e.g. S416, barley; S420, potato) (Obenauer et al, 2003;Sowokinos et al, 2004). A fine regulation of UGPase by those mechanisms would not be entirely surprising, given that UDP-Glc (a substrate/product of the enzyme) is not only a key precursor for polysaccharides, but also a signaling molecule (Koch, 1996;Higuita et al, 2003).…”

Section: Perspectivesmentioning

confidence: 99%

Toward a blueprint for UDP-glucose pyrophosphorylase structure/function properties: homology-modeling analyses

et al. 2004

View full text Add to dashboard Cite

UDP-glucose pyrophosphorylase (UGPase) is an important enzyme of synthesis of sucrose, cellulose, and several other polysaccharides in all plants. The protein is evolutionarily conserved among eukaryotes, but has little relation, aside from its catalytic reaction, to UGPases of prokaryotic origin. Using protein homology modeling strategy, 3D structures for barley, poplar, and Arabidopsis UGPases have been derived, based on recently published crystal structure of human UDP-N-acetylglucosamine pyrophosphorylase. The derived 3D structures correspond to a bowl-shaped protein with the active site at a central groove, and a C-terminal domain that includes a loop (I-loop) possibly involved in dimerization. Data on a plethora of earlier described UGPase mutants from a variety of eukaryotic organisms have been revisited, and we have, in most cases, verified the role of each mutation in enzyme catalysis/regulation/structural integrity. We have also found that one of two alternatively spliced forms of poplar UGPase has a very short I-loop, suggesting differences in oligomerization ability of the two isozymes. The derivation of the structural model for plant UGPase should serve as a useful blueprint for further function/structure studies on this protein.

show abstract

Molecular cloning and sequence variation of UDP-glucose pyrophosphorylase cDNAs from potatoes sensitive and resistant to cold sweetening

Cited by 26 publications

References 37 publications

Proteomic analysis of the potato tuber life cycle

Proteomic analysis of the potato tuber life cycle

Regulation of UDP-glucose pyrophosphorylase isozyme UGP5 associated with cold-sweetening resistance in potatoes

Toward a blueprint for UDP-glucose pyrophosphorylase structure/function properties: homology-modeling analyses

Contact Info

Product

Resources

About