Proteomic analysis of the potato tuber life cycle

Lehesranta, Satu; Davies, Howard V.; Shepherd, L.V.T.; Koistinen, Kaisa M.; Massat, Nathalie; Nunan, Naoise; McNicol, James W.; Kärenlampi, Sirpa

doi:10.1002/pmic.200600383

Cited by 75 publications

(68 citation statements)

References 45 publications

(64 reference statements)

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“…In potato, proteome studies have been carried out mainly on tuber tissue (Bauw et al 2006;Delaplace et al 2005;Lehesranta et al 2005Lehesranta et al , 2006 and in connection with investigation on the action of phytopathogens such as Pectobacterium atrosepticum (Coulthurst et al 2006, Mattinen et al 2007) and the aphid Macrosiphum euphorbiae (Nguyen et al 2007). The application of protein expression studies for cryopreservation is a new research area.…”

Section: Biochemical Analysis and Proteome Researchmentioning

confidence: 99%

Potato Shoot Tip Cryopreservation. A Review

2010

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Potato is one of the most important crops worldwide. Genetic resources of potato (Solanum tuberosum L. ssp. tuberosum) and related cultivated species are conserved through storage of tubers, in vitro plants and in cryopreservation. Cryopreservation, storage in or above liquid nitrogen, is the best option to maintain vegetatively propagated plants in the long term. The present review gives comprehensive information about various cryopreservation techniques for potato published from 1977 until the present. It discusses factors that affect the process and success of cryopreservation, such as donor culture conditions, preculture, cooling, warming and post-culture treatments. Studies are presented that analyse the histological and ultrastructural changes after different cryopreservation steps and the morphological pathways during regeneration of plants after rewarming. The maintenance of genetic stability in potato after cryopreservation has also been demonstrated by various phenotypic and molecular methods. The first thermal analyses on potato shoot tips are presented using differential scanning calorimetry to analyse the state of water during cooling and warming. Biochemical analyses of different compounds, such as soluble sugars and proteins, have been performed to understand and improve existing cryogenic methods. Potato is an example where successful virus elimination has been obtained via cryopreservation of shoot tips (cryotherapy). There are already cryopreserved collections of potato shoot tips in

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Section: Biochemical Analysis and Proteome Researchmentioning

confidence: 99%

Potato Shoot Tip Cryopreservation. A Review

2010

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“…Comparative protein profiling analysis has also been carried out in potatoes using different varieties, environmental conditions and genetically modified lines [59].…”

Section: Proteomicsmentioning

confidence: 99%

Profiling of Genetically Modified Organisms Using Omics Technologies

Valdés

Simó

Ibáñez

et al. 2014

Comprehensive Analytical Chemistry

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Strict regulations including risk assessment, labeling, traceability and marketing have been established due to the controversial safety aspects of genetically modified organisms (GMOs).One of the main polemic issues associated with GMOs safety are the possible unintended effects, defined as effects that go beyond the primary expected effects of the genetic modification. In order to effectively investigate the potential adverse effects on the human health, including the existence or not of unintended effects, new analytical tools are needed to facilitate comprehensive compositional studies of GMOs. In this context, profiling technologies have the potential to provide valuable information regarding GMOs composition that can be useful for characterization, traceability and even GMO detection. In this chapter, the application of the main -omics technologies (transcriptomics, proteomics, and metabolomics) in combination with bioinformatics and chemometrics tools to GMO profiling are discussed.

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“…Gel matching in the present study was based on a reference gel image, created by Progenesis Same Spots (Nonlinear Dynamics) during the image analysis procedure. The reference gel image was used to compare the migration of spots with three previously published potato tuber proteome maps that had been generated by the same 2-DE gel methods to separate protein spots [14,31,32]. Of these three studies, one of them used the same potato variety as used in the present study-Santé [14].…”

Section: Protein Inference By Gel Matchingmentioning

confidence: 99%

“…Of the protein spots whose abundance was significantly (p < 0.01) affected by fertilization and / or previous crop management (PCM) treatments, the function of 47 was inferred by gel matching using 3 published potato tuber proteome maps [14,31,32] and the reference proteome map generated in this study ( Figure 5). Proteins fell into six distinct functional groups: ATP synthesis/binding (2 protein spots), disease/defense/stress response (16 protein spots), energy production-glycolysis (11 protein spots), metabolism (3 protein spots), protein transport/destination/storage (11 protein spots), and protein breakdown (proteolysis/catabolism, 2 protein spots) ( Table 4).…”

Section: Protein Inference By Gel Matchingmentioning

confidence: 99%

Effects of Previous Crop Management, Fertilization Regime and Water Supply on Potato Tuber Proteome and Yield

et al. 2013

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There is increasing concern about the sustainability and environmental impacts of mineral fertilizer use in agriculture. Increased recycling of nutrients via the use of animal and green manures and fertilizers made from domestic organic waste may reduce reliance on mineral fertilizers. However, the relative availability of nutrients (especially nitrogen) is lower in organic compared to mineral fertilizers, which can result in significantly lower yields in nutrient demanding crops such as potato. It is therefore important to gain a better understanding of the factors affecting nutrient use efficiency (yield per unit fertilizer input) from organic fertilizers. Here we show that (a) previous crop management (organic vs. conventional fertilization and crop protection regimes), (b) organic fertilizer type and rate (composted cattle manure vs. composted chicken manure pellets) and (c) watering regimes (optimized and restricted) significantly affected leaf chlorophyll content, potato tuber N-concentration, proteome and yield. Protein inference by gel matching indicated several functional groups significantly affected by previous crop management and organic fertilizer type and rate, including stress/defense response, glycolysis and protein destination and storage. These results indicate genomic pathways controlling crop responses (nutrient use efficiency and yield) according to contrasting types and rates of organic fertilizers that can be linked to the respective encoding genes.

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Proteomic analysis of the potato tuber life cycle

Cited by 75 publications

References 45 publications

Potato Shoot Tip Cryopreservation. A Review

Potato Shoot Tip Cryopreservation. A Review

Profiling of Genetically Modified Organisms Using Omics Technologies

Effects of Previous Crop Management, Fertilization Regime and Water Supply on Potato Tuber Proteome and Yield

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