Molecular cloning and functional expression of the Penaeus monodon 5-HT receptor

Ongvarrasopone, Chalermporn; Roshorm, Yaowaluck Maprang; Somyong, Suthasinee; Pothiratana, Chetsada; Petchdee, Soontaree; Tangkhabuanbutra, Jarasporn; Sophasan, Samaisukh; Panyim, Sakol

doi:10.1016/j.bbaexp.2006.07.004

Cited by 39 publications

(19 citation statements)

References 34 publications

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“…In P. monodon, 5-HT concentrations in the ovary appear to be higher in the late stages (III and IV), and 5-HT-ir is more intense in mature oocytes (Oc4; Wongprasert et al 2006). Additionally, 5-HT1 receptors are also expressed in the oocyte membranes at these stages (Ongvarrasopone et al 2006). These data indicate that 5-HT plays a direct role in regulating oocyte maturation.…”

Section: -Ht-irmentioning

confidence: 84%

Distribution and changes of serotonin and dopamine levels in the central nervous system and ovary of the Pacific white shrimp, Litopenaeus vannamei, during ovarian maturation cycle

et al. 2011

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We investigated changes in serotonin (5-HT) and dopamine (DA) levels and in their distribution patterns in the central nervous system (CNS) and ovary during the ovarian maturation cycle in the Pacific white shrimp, Litopenaeus vannamei. The concentrations of these two neurotransmitters were determined by using high performance liquid chromatography with electrochemical detection. The 5-HT concentration exhibited a gradual increase in the brain and thoracic ganglia during early ovarian stages I, II, and III, reaching a maximum at the mature ovarian stage IV, whereas DA showed its highest concentration at ovarian stage II in the brain and thoracic ganglia and then declined to its lowest concentration at ovarian stage IV. In the ovaries, 5-HT was lowest at ovarian stage I and gradually increased to a peak at ovarian stage IV. Conversely, the concentration of DA was highest at ovarian stages I and II and lowest at ovarian stage IV. In the brain, 5-HT immunoreactivity (-ir) from stage IV and DA-ir from stage II were distributed extensively in neurons of clusters 6, 11, and 17, in fibers, and in the anterior and posterior medial protocerebral, olfactory, antenna II, and tegumentary neuropils. In the circumesophageal, subesophageal, thoracic, and abdominal ganglia, both 5-HT-ir and DA-ir were detected in neuropils and surrounding neurons and fibers. 5-HT-ir and DA-ir were more intense in the thoracic ganglia than in other parts of the CNS. In the ovary, 5-HT-ir exhibited high intensity in late oocytes, whereas DA-ir was more intense in early oocytes. Thus, opposing changes occur in the levels of these two neurotransmitters and in their specific localizations in the CNS and ovary during ovarian maturation, indicating their important involvement in female reproduction.

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Section: -Ht-irmentioning

confidence: 84%

Distribution and changes of serotonin and dopamine levels in the central nervous system and ovary of the Pacific white shrimp, Litopenaeus vannamei, during ovarian maturation cycle

et al. 2011

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“…Serotonin (5-hydroxytryptamine, 5-HT) injection has been demonstrated to induce ovarian maturation in penaeids 27, 28 and enhance release of CHH in the crayfish, Procambarus clarkia 29. Ongvarrasopone et al ., (2006) 30 have cloned a putative serotonin receptor from the ovary of P. monodon and further determined its expression in all tissues examined including the ES. It is possible that elevated arrestin levels at P and 2 stages in the wild-caught eyestalk indicate changes in GPCR related signalling activity at these stages, potentially associated with regulation of eyestalk neuropeptide release.…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Profiling of the Cephalothorax and Eyestalk in Penaeus Monodon during Ovarian Maturation

Brady¹,

Elizur²,

Williams³

et al. 2012

Int. J. Biol. Sci.

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In crustaceans, a range of physiological processes involved in ovarian maturation occurs in organs of the cephalothorax including the hepatopancrease, mandibular and Y-organ. Additionally, reproduction is regulated by neuropeptide hormones and other proteins released from secretory sites within the eyestalk. Reproductive dysfunction in captive-reared prawns, Penaeus monodon, is believed to be due to deficiencies in these factors. In this study, we investigated the expression of gene transcripts in the cephalothorax and eyestalk from wild-caught and captive-reared animals throughout ovarian maturation using custom oligonucleotide microarray screening. We have isolated numerous transcripts that appear to be differentially expressed throughout ovarian maturation and between wild-caught and captive-reared animals. In the cephalothorax, differentially expressed genes included the 1,3-β-D-glucan-binding high-density lipoprotein, 2/3-oxoacyl-CoA thiolase and vitellogenin. In the eyestalk, these include gene transcripts that encode a protein that modulates G-protein coupled receptor activity and another that encodes an architectural transcription factor. Each may regulate the expression of reproductive neuropeptides, such as the crustacean hyperglycaemic hormone and molt-inhibiting hormone. We could not identify differentially expressed transcripts encoding known reproductive neuropeptides in the eyestalk of either wild-caught or captive-reared prawns at any ovarian maturation stage, however, this result may be attributed to low relative expression levels of these transcripts. In summary, this study provides a foundation for the study of target genes involved in regulating penaeid reproduction.

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“…In crustaceans, for instance, 5-HT appears to be involved in ovarian maturation, and a cloned and sequenced 5-HT 1 -like receptor was found to be expressed not only in gonadal somatic cells but also in oocytes themselves during the last steps of gametogenesis (Ongvarrasopone et al, 2006). There thus appears to be local serotonergic regulation of spawning and possibly also of oocyte meiotic maturation and early embryonic cleavage cycles (Buznikov et al, 2001) in diverse lower invertebrate phyla.…”

Section: Widespread Serotonergic Regulation Of Female Reproduction Inmentioning

confidence: 97%

Local serotonergic signaling in mammalian follicles, oocytes and early embryos

Dubé

Amireault

2007

Life Sciences

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Molecular cloning and functional expression of the Penaeus monodon 5-HT receptor

Cited by 39 publications

References 34 publications

Distribution and changes of serotonin and dopamine levels in the central nervous system and ovary of the Pacific white shrimp, Litopenaeus vannamei, during ovarian maturation cycle

Distribution and changes of serotonin and dopamine levels in the central nervous system and ovary of the Pacific white shrimp, Litopenaeus vannamei, during ovarian maturation cycle

Gene Expression Profiling of the Cephalothorax and Eyestalk in Penaeus Monodon during Ovarian Maturation

Local serotonergic signaling in mammalian follicles, oocytes and early embryos

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