Molecular Cloning and Functional Expression of cDNA Encoding a Cysteine Proteinase Inhibitor, Cystatin, from Job's Tears (Coix lacryma-jobiL. var.Ma-yuenStapf)

A cold inducible multidomain cystatin from winter wheat inhibits growth of the snow mold fungus, Microdochium nivale

“…It has been reported that a number of plant cystatins were able to inhibit various proteinases such as papain, cathepsin, ficin and chrymopapain. Papain inhibition has been confirmed for cystatins that were isolated from rice , corn (Abe et al 1994), barley (Gaddour et al 2001), Job's tears (Yoza et al 2002), sugarcane (Oliva et al 2004), sunflower (Kouzuma et al 1996), carnation (Sugawara et al 2002 and chestnut (Pernas et al 1998). We analyzed papain inhibition activity of a recombinant wheat cystatin and the calculated value of Ki (5.8·10 À7 M) was comparable to the other plant cystatins (10 À7 -10 À9 M; Brzin et al 1998;Kouzuma et al 1996).…”

“…Papain inhibition by mTaMDC1 was determined by using BAPA (benzoyl-L-arginine-p-nitroanilide) as a substrate according to the previously described method (Yoza et al 2002). Briefly, inhibition activity of different concentrations of mTaMDC1 to 0.1 mg/ml papain (Sigma) was analyzed using activation buffer (100 mM Sodium phosphate, pH 6.8, 2.5 mM DTT, 10 mM EDTA) and 10 mM BAPA (Peptide Institute Inc., Osaka, Japan) as a substrate.…”

Heterologous Expression of Corn Cystatin in Soybean and Effect on Growth of the Stink Bug

“…One group of such inhibitors, cystatins, inhibit such cysteine proteases as papain and cathepsins, and have been isolated from a variety of food crops, including rice, 1) corn (maize), 2) wheat, 3) Job's tears, 4) soybean, 5) and papaya. 6) Cystatins seem to regulate phytophysiological events such as germination and apoptosis by inhibiting endogenous cysteine proteinases.…”

“…We selected three cereal cystatins, OC1 (P09229), 1) CC1 (P31726), 2) and Job's tears cystatin (JTC, BAB21558), 4) whose structures differ considerably from that of soybean cystatin, 5) as candidates. Their deduced amino acid sequences have hydrophobic residues in the N-terminal region resembling a signal peptide, suggesting that they are vacuole-secretory proteins 7) (Fig.…”

Avidin Expressed in Transgenic Rice Confers Resistance to the Stored-Product Insect PestsTribolium confusumandSitotroga cerealella

“…We are interested in protecting crops during storage from attack by insect pests using insect growth-inhibiting proteins, e.g., proteinase inhibitors. [1][2][3] If genes coding for these proteins are introduced into crops, the transformant might produce enough of the protein to provide resistance against pests. There are many biocidal proteins that occur in nature that are potential biopesticides for stored grain protection.…”