Avidin Expressed in Transgenic Rice Confers Resistance to the Stored-Product Insect Pests<i>Tribolium confusum</i>and<i>Sitotroga cerealella</i>

Yoza, Koh-Ichi; Imamura, Taro; Kramer, Karl J.; Morgan, Thomas D.; Nakamura, Sumiko; Akiyama, Kazuo; Kawasaki, Shinji; Takaiwa, Fumio; Ohtsubo, Ken’ichi

doi:10.1271/bbb.69.966

Cited by 33 publications

(19 citation statements)

References 17 publications

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“…Different sizes (1.3 kb, 2.3 kb and 2.4 kb) of the GluB-1 promoter have been used in various transgenic approaches to produce higher levels of ectopic gene expressions. These include: β-carotene in golden rice, human lactoferrin (hLF) gene in Bulgarian barley, soybean ferritin gene in rice, over expression of GABA in transgenic rice cell lines, artificial avidin in rice and over expressed TPC7 antigen in rice (Goto et al, 1999;Katsube et al, 1999;Yoza et al, 2005;Kamenarova et al, 2007;Akama et al, 2009;Wang et al, 2013). All GluB-1 promoters showed seed specific expression in the aleurone and sub aleurone layers (Wu et al, 1998).…”

Section: December 2019mentioning

confidence: 99%

Cloning and characterisation of an endosperm specific glutelin B1 promoter from Sri Lankan rice (Oryza sativa L. ssp. indica) variety Bg 250

Kajendran

Chandrasekharan

Hettiarachchi

et al. 2019

J. Natn. Sci. Foundation Sri Lanka

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Glutelins are the primary source of energy storage in the endosperm of rice grains. Among the glutelin promoters, Glutelin B-1 (GluB-1) is widely studied and used in transgenic rice plants to express recombinant proteins in the endosperm. In this study, three regions: 350 bp, 1308 bp and 2300 bp of the GluB-1 promoter were PCR amplified from the genomic DNA of Bg 250 rice variety. The amplified fragments were cloned into pGEM®-T Easy vector for characterisation of GluB-1 promoter. Each region of GluB-1 promoter was separately cloned into the promoterless binary vector pCAMBIA1391Z harbouring the β-glucuronidase (GUS) reporter gene. Putative transgenic plants were generated by Agrobacterium-mediated gene transformation and confirmed by PCR using nopaline synthase terminator primers. All GluB-1 promoter constructs showed expression of the GUS gene in the endosperm of T 0 transgenic plant seeds. The 1308 bp GluB-1 promoter revealed the highest expression as determined by the GUS assay. This indicates the potential of this promoter for expression of recombinant proteins in rice endosperm.

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Section: December 2019mentioning

confidence: 99%

Cloning and characterisation of an endosperm specific glutelin B1 promoter from Sri Lankan rice (Oryza sativa L. ssp. indica) variety Bg 250

Kajendran

Chandrasekharan

Hettiarachchi

et al. 2019

J. Natn. Sci. Foundation Sri Lanka

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“…25,26) Here, we optimized the pattern of codon use by the tamavidin 2 gene for high-level expression in human cells.…”

Section: )mentioning

confidence: 99%

High-level expression of tamavidin 2 in human cells by codon-usage optimization

Takakura

Katayama

Nagata

2015

Bioscience, Biotechnology, and Biochemistry

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Tamavidin 2 is a fungal protein that binds to biotin with an extremely high affinity. Tamavidin 2 is superior to avidin or streptavidin in terms of its low-level non-specific binding and high-level thermal stability. However, the gene for tamavidin 2 is highly expressed in Escherichia coli but not in mammalian cells, restricting its application as an affinity tag in mammalian cells. Here, we optimized the codon usage of tamavidin 2 for human cells and found that the resultant mutant expressed tamavidin 2 at approximately 30-fold higher level compared with the native gene. The protein thus produced in human cells could be purified by iminobiotin affinity chromatography, bound tightly to biotin, and was stable at high temperature (82 °C). This powerful technology for high-level expression of tamavidin 2 in mammalian cells will be of value in evaluating various fusion proteins produced in mammalian cells for numerous applications.

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“…Furthermore, avidin is detrimental to other potato pests, such as Colorado potato beetle (Cooper et al 2006). It is also effective against number of lepidopteran and coleopteran pests in other crops; therefore, it may negatively impact other pests of the potato like wireworms, Elateridae spp., Andean potato weevil, Premnotrypes suturicallus Kuschel, and variegated cutworms, Peridroma saucia (Hübner) (Kramer et al 2000Burgess et al 2002;Yoza et al 2005).…”

Section: Potato Tuberworm Bioassaysmentioning

confidence: 99%

“…Avidin denatures during cooking and loses its ability to bind to biotin (Durance 1991). Less than 3% of the avidin in transgenic rice was able to bind to biotin after cooking (Yoza et al 2005). Similarly, potato is typically cooked before being consumed.…”

Section: Potato Tuberworm Bioassaysmentioning

confidence: 99%

Enhanced Resistance to Control Potato Tuberworm by Combining Engineered Resistance, Avidin, and Natural Resistance Derived from, Solanum Chacoense

et al. 2008

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Potato tuberworm, Phthorimaea operculella (Zeller), is a destructive insect pest of potato, Solanum tuberosum (L.), found primarily in tropical and sub-tropical regions. It has recently become established in the northwestern United States. Avidin is a natural protein found in chicken (Gallus gallus L.) egg whites that has insecticidal properties against a number of lepidopteran and coleopteran pests. Biotin is a cofactor of carboxylases that are required for many important processes like lipogenesis, gluconeogensis, fatty acid and amino acid catabolism. Without biotin, an insect's growth is severely stunted, eventually leading to death. Avidin binds and sequesters biotin, limiting its availability during insect growth and development. Previous studies have demonstrated that avidin is effective against potato tuberworm. We sought to elevate resistance by combining avidin with natural host plant resistance factors from the wild species Solanum chacoense Bitter. We expressed avidin in two potato lines: MSE149-5Y, a susceptible potato line, and ND5873-15, a line with S. chacoense-derived resistance. The avidin expression was determined by ELISA to be 10.6 μM in MSE75.7 (avidin) and 12.5 μM in ND75.3 (avidin + S. chacoense-derived) in the tuber. Potato tuberworm bioassays were performed on MSE149-5Y, MSE75.7, ND5873-15, and ND75.3. Mortality was measured after 28 d. Mortality of larvae fed on MSE149-5Y (susceptible) did not differ significantly compared to the mortality of larvae fed on MSE75.7 (avidin) or ND5873-1 (S. chacoense-derived). Significantly higher mortality (98%) was observed for larvae fed on ND75.3 (avidin + S. chacoense-derived) tubers than for larvae fed on MSE149-5Y (susceptible). The present study is the first report of combining avidin with natural host plant resistance factors against potato tuberworm. Expressing avidin in combination with natural host plant resistance may be of value in managing potato tuberworm. Resumen El gusano del tubérculo Phthomaea operculella (Zeller) es una plaga destructiva de papa Solanum tuberosum L., se encuentra principalmente en las regiones tropical y subtropical. Se le ha encontrado recientemente en los estados del noroeste de los EE.UU. La avidina es una proteína que se halla en la clara de huevo de las aves de corral (Gallus gallus L.) que tiene propiedades insecticidas contra ciertos lepidópteros y coleópteros. La biotina es un cofactor de las carboxilasas requerido para muchos procesos importantes como la lipogénesis, gluconogésis y el catabolismo de los ácidos grasos y aminoácidos. Sin la biotina, el crecimiento del insecto es severamente afectado conduciéndolo eventualmente a la muerte. La avidina une y secuestra a la biotina, limitando su disponibilidad durante el crecimiento y desarrollo del insecto. Estudios previos han demostrado que la avidina es efectiva contra el gusano del tubérculo de papa. Buscamos elevar la resistencia mediante la combinación de avidina con factores de resistencia natural de plantas hospedantes provenientes de Solanum chacoense Bit...

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Avidin Expressed in Transgenic Rice Confers Resistance to the Stored-Product Insect PestsTribolium confusumandSitotroga cerealella

Cited by 33 publications

References 17 publications

Cloning and characterisation of an endosperm specific glutelin B1 promoter from Sri Lankan rice (Oryza sativa L. ssp. indica) variety Bg 250

Cloning and characterisation of an endosperm specific glutelin B1 promoter from Sri Lankan rice (Oryza sativa L. ssp. indica) variety Bg 250

High-level expression of tamavidin 2 in human cells by codon-usage optimization

Enhanced Resistance to Control Potato Tuberworm by Combining Engineered Resistance, Avidin, and Natural Resistance Derived from, Solanum Chacoense

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