Molecular cloning and functional characterization of a human 5-HT1B serotonin receptor: A homologue of the rat 5-HT1B receptor with 5-HT1D-like pharmacological specificity

Hamblin, Mark W.; Metcalf, Mark A.; McGuffin, Robert W.; Karpells, Sharon T.

doi:10.1016/0006-291x(92)90654-4

Cited by 110 publications

(48 citation statements)

References 23 publications

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“…For the 5-HT 1B studies, three oligonucleotides corresponding to base pairs 1343-1382, 1630-1668, and 1790-1829 of the rat 5-HT 1B clone, MG11B (Hamblin et al, 1992) were used. For 5-HT 1A probes, one oligonucleotide corresponding to base pairs 1110-1151 was used (Neumaier et al, 2000).…”

Section: Nih-pa Author Manuscriptmentioning

confidence: 99%

Chronic low dose ovine corticotropin releasing factor or urocortin II into the rostral dorsal raphe alters exploratory behavior and serotonergic gene expression in specific subregions of the dorsal raphe

et al. 2007

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Corticotropin releasing factor (CRF) family peptides play key roles in integrating neural responses to stress. Both major CRF receptors have been pharmacologically identified in the dorsal raphe nucleus (DRN), a stress sensitive and internally heterogeneous nucleus supplying many forebrain regions with serotonergic input. Despite the involvement of chronic stress and serotonergic dysfunction in human mood and anxiety disorders, little is known about the effects of chronic CRF receptor activation on the DRN. We infused ovine CRF (1ng/hr), urocortin II (UCNII, 1ng/hr), or vehicle alone into rat DRN over 6 days. During infusion, animals were allowed to freely explore an open field for 15 minutes on each of two days, with the addition of a novel object on the second day. Following behavioral testing, 5-HT 1A , 5-HT 1B , serotonin transporter (SERT), and tryptophan hydroxylase-2 (Tph2) expression were examined through the DRN by in situ hybridization. Ovine CRF infusion resulted in significantly decreased novel object touches, climbs, as well as increased latency to first novel object contact. UCNII had a similar but less dramatic effect, decreasing only climbing behavior. Both ovine CRF and UCNII blunted the decrease in corner time expected on reexposure to the open field. Both peptides also produced regionally specific changes in gene expression: 5-HT 1A expression was increased 30% in the mid-rostral ventromedial DRN, while SERT was decreased by 30% in the mid-caudal shell dorsomedial DRN. There also appeared to be a shift in the relative level of Tph2 expression between the ventromedial and core dorsomedial DRN at the mid-rostral level. Changes in 5-HT 1A , SERT, and relative Tph2 mRNA abundance were correlated with novel object exploration. These findings suggest chronic intra-DRN administration of CRF agonists decreases exploratory behavior, while producing subregionally limited changes in serotonergic gene expression. These studies may be relevant to mechanisms underlying behavioral changes after chronic stress. NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author ManuscriptDepression and anxiety disorders are among the leading causes of morbidity, mortality, and disability in the United States (Greenberg et al., 2003). They are often comorbid, and both are highly associated with chronic exposure to stress. Multiple lines of evidence suggest that chronic and inescapable stress produce long lasting effects on brain function and behavior that play a prominent role in the development, maintenance, and recurrence of both depression and a variety of anxiety disorders (Gulley and Nemeroff, 1993, Arborelius et al., 1999, Ressler and Nemeroff, 2000.Altered serotonin neurotransmission appears to be a central mechanism inducing both depressive (Maes and Meltzer, 1995) and anxiety disorders (Ressler and Nemeroff, 2000). The majority of serotonergic fibers to forebrain regions thought to be involved in mood and anxiety regulation arise from the dorsal raphe nucleus (DRN) and median raphe nucleus (MRN) ...

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Section: Nih-pa Author Manuscriptmentioning

confidence: 99%

Chronic low dose ovine corticotropin releasing factor or urocortin II into the rostral dorsal raphe alters exploratory behavior and serotonergic gene expression in specific subregions of the dorsal raphe

et al. 2007

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“…With the exception of the ligand-gated channel 5-HT3 receptor, all 5-HT receptors belong to the super-family of G-protein coupled receptors containing a predicted seventransmembrane domain structure. The 5-HT1 receptor class is subdivided into the 5-HTIA (Fargin et al, 1988), 5-HTlB (5-HTIDP) (Voigt et al, 1991;Adham et al, (1992); Bruinvels et al, 1992;Hamblin et al, (1992); Maroteaux et al, (1992)), 5-HTID (5-HTlDa) , 5-htIE and 5-htlF (Adham et al, 1993) subtypes, all of which are negatively coupled to adenylate cyclase activity. Three 5-HT2 receptor subtypes, each coupled to phosphatidylinositol-4,5-biphosphate hydrolysis, are at present defined: the 5-HT2A (Pritchett et al, 1988), 5-HT2C (previously 5-HT1c, Julius et al, 1988), and 5-HT2B (Foguet et al, 1992;Kursar et al, 1992) receptors.…”

Section: Introductionmentioning

confidence: 99%

Sequential onset of three 5‐HT receptors during the 5‐hydroxytryptaminergic differentiation of the murine 1C11 cell line

Kellermann

Loric

Maroteaux

et al. 1996

British J Pharmacology

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“…In Fig. 2, several specific key amino acid residues can be recognised: residues involved in a disulfide bridge (121Cys and 198Cys, SWISSPROT website http://us.expasy.org/sprot/; Swissprot accession number P28222), and potential sites for palmitoylation (387Cys), N-linked glycosylation (24Asp and 31Asp), phosphorylation by protein kinase C (76Thr, 157Ser, 161Thr, 236Ser and 246Thr), phosphorylation by tyrosine kinase (371Ser), and phosphorylation by protein kinase A (251Thr and 312Thr) Hamblin et al, 1992;Maroteaux et al, 1992).…”

Section: Canine Htr1b Sequencementioning

confidence: 99%

“…Like most serotonin receptors it is a member of the family of G-protein coupled receptors. The protein has seven transmembrane regions and its activity is mediated by G-proteins that inhibit adenylate cyclase (Hamblin et al, 1992;Maroteaux et al, 1992). 5-HT 1B receptors can be found both pre-and postsynaptically on a variety of neurons in several brain areas, and they modulate the release of both serotonin and other neurotransmitters (Hartig, 1997;Hoyer et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…5-HT 1B receptors can be found both pre-and postsynaptically on a variety of neurons in several brain areas, and they modulate the release of both serotonin and other neurotransmitters (Hartig, 1997;Hoyer et al, 2002). The gene encoding serotonin receptor 1B (htr1B) has no introns and in man 16 polymorphisms have been discovered in its coding and surrounding DNA sequence Hamblin et al, 1992;Maroteaux et al, 1992;Sanders et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of the canine serotonin receptor 1B gene (htr1B)

Berg¹,

Imholz²,

Versteeg³

et al. 2004

Gene

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The serotonin receptor 1B gene (htr1B) has been suggested to be implicated in mental disorders in both humans and other species. We have isolated a canine bacterial artificial chromosome (BAC) clone containing htr1B, revealed the coding and surrounding DNA sequence of canine htr1B and designed primer sets for genomic sequencing of the gene. A mutation scan in 10 dogs revealed five single nucleotide polymorphisms in the htr1B coding sequence. By random sequencing of subclones of the BAC a polymorphic microsatellite repeat was found. We found evidence for at least four extended haplotypes in six dogs of the same breed. The chromosomal localization of the gene was confirmed by fluorescence in situ hybridisation and radiation hybrid mapping. This work provides a starting point for mutation scans and association studies on dogs with behavioural problems. D

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Molecular cloning and functional characterization of a human 5-HT1B serotonin receptor: A homologue of the rat 5-HT1B receptor with 5-HT1D-like pharmacological specificity

Cited by 110 publications

References 23 publications

Chronic low dose ovine corticotropin releasing factor or urocortin II into the rostral dorsal raphe alters exploratory behavior and serotonergic gene expression in specific subregions of the dorsal raphe

Chronic low dose ovine corticotropin releasing factor or urocortin II into the rostral dorsal raphe alters exploratory behavior and serotonergic gene expression in specific subregions of the dorsal raphe

Sequential onset of three 5‐HT receptors during the 5‐hydroxytryptaminergic differentiation of the murine 1C11 cell line

Isolation and characterization of the canine serotonin receptor 1B gene (htr1B)

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