Isolation and characterization of the canine serotonin receptor 1B gene (htr1B)

Berg, Linda van den; Imholz, Sandra; Versteeg, Serge A.; Leegwater, P.A.J.; Zijlstra, C.; Bosma, Anneke A.; Oost, B.A. van

doi:10.1016/j.gene.2003.10.028

Cited by 12 publications

(6 citation statements)

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“…Although the draft sequence of canine genome was unveiled on the website of National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov/genome/ guide/dog/) on July 2004, only a few genetic markers that will be related to canine behavioral traits have been listed up [12,14,15,19,20,25,26,37]. This situation is possibly a result of the lack of researchers devoted to this area of science.…”

Section: Discussionmentioning

confidence: 99%

Canine Tyrosine Hydroxylase (TH) Gene and Dopamine .BETA.-Hydroxylase (DBH) Gene: Their Sequences, Genetic Polymorphisms, and Diversities among Five Different Dog Breeds

Takeuchi

Hashizume

Chon

et al. 2005

The Journal of Veterinary Medical Science

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ABSTRACT. Dopamine and noradrenaline are catecholamine neurotransmitters that are produced by biosynthetic enzymes such as tyrosine hydroxylase (TH) and dopamine β -hydroxylase (DBH). As a first step to elucidate the genetic background of canine behavioral traits, we selected these genes as targets and sequenced these canine genes, and found that both were highly homologous with those of human beings. Then brain cDNAs derived from ten unrelated Beagles were used to search for polymorphisms in these genes. Four single nucleotide polymorphisms (SNPs) (C97T, G168A, G180A and C264T), one of which (C97T) will cause amino acid substitution in the TH gene, and two SNPs (C789A and A1819G), both of which will cause amino acid substitutions in the DBH gene were identified. The allelic frequencies among five dog breeds (47 Golden Retrievers, 41 Labrador Retrievers, 40 Malteses, 26 Miniature Schnauzers, and 39 Shibas) were examined and found to have significant variation between them with regards to all these SNPs, except for C97T in the TH gene and A1819G in the DBH gene. The polymorphisms of C97T and A1819G were found only in the Shiba. The present results suggest that the polymorphisms of the genes encoding catecholamine biosynthetic enzymes may become important markers for examining the genetic background of behavioral characteristics in dogs. KEY WORDS: breed difference, DBH, dog, polymorphism, TH.J. Vet. Med. Sci. 67(9): 861-867, 2005 Since a genetic polymorphism related to the dopamine D4 receptor (DRD4) was postulated to be related to the "novelty seeking" behavior [2, 9], numerous genetic markers for such behavioral traits have been researched in the human psychiatric field [1,5,6,17,23,30]. Most of these markers are on genes coding for neurotransmitters, neuropeptides, neurotropic factors or transcription factors, as well as their receptors, synthetic enzymes and metabolic enzymes. Such research is based on the fundamental concept that one's personality is formed from the activity and/or turnover of such components of the hypothalamo-limbic system. Although this system is thought to be conserved in mammals, few polymorphic markers that could be responsible for the behavioral traits or temperament in the other mammals have been listed up. This situation is no exception in case of dogs even though we can now access various canine genome resources for linkage analysis, i.e. the 1 Mb resolution radiation hybrid map of the canine genome [10], the map of 4249 genetic markers featuring one marker every 900kb [3]. Therefore we have tried to search genetic markers that would be related to the behavioral traits in dogs and so far found some single nucleotide polymorphisms (SNPs) in neurotransmitter-related genes [12,19,20].In this study tyrosine hydroxylase (TH) and dopamine β -hydroxylase (DBH) genes were selected for their possible relation to canine behavioral traits. We sequenced these genes, and searched for genetic polymorphisms in these genes. The TH and DBH are biosynthetic enzymes for the synthesis of catecholam...

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Section: Discussionmentioning

confidence: 99%

Canine Tyrosine Hydroxylase (TH) Gene and Dopamine .BETA.-Hydroxylase (DBH) Gene: Their Sequences, Genetic Polymorphisms, and Diversities among Five Different Dog Breeds

Takeuchi

Hashizume

Chon

et al. 2005

The Journal of Veterinary Medical Science

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“…Many of these clones, which were FISH mapped to determine their cytogenetic location, contained genes associated with disease (e.g. van de Sluis et al, 1999Sluis et al, , 2001Wagner et al, 1999;Thomas et al, 2001a;Lozier et al, 2002;Rak et al, 2002Rak et al, , 2003van den Berg et al, 2003van den Berg et al, , 2004Wagner, 2003;Klukowska et al, 2004a, b;Stabej et al, 2004Stabej et al, , 2005Debenham et al, 2005;Philipp et al, 2005; also see http://faculty.vetmed.ucdavis. edu/Faculty/lalyons/Sites/CompGenBACsDOG.htm) and cancer (Thomas et al, 2003a, b).…”

Section: The Role Of Canine Molecular Cytogenetics In Genome Mappingmentioning

confidence: 99%

Canine cytogenetics – from band to basepair

Breen

2008

Cytogenet Genome Res

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Humans and dogs have coexisted for thousands of years, during which time we have developed a unique bond, centered on companionship. Along the way, we have developed purebred dog breeds in a manner that has resulted unfortunately in many of them being affected by serious genetic disorders, including cancers. With serendipity and irony the unique genetic architecture of the 21st century genome of Man’s best friend may ultimately provide many of the keys to unlock some of nature’s most intriguing biological puzzles. Canine cytogenetics has advanced significantly over the past 10 years, spurred on largely by the surge of interest in the dog as a biomedical model for genetic disease and the availability of advanced genomics resources. As such the role of canine cytogenetics has moved rapidly from one that served initially to define the gross genomic organization of the canine genome and provide a reliable means to determine the chromosomal location of individual genes, to one that enabled the assembled sequence of the canine genome to be anchored to the karyotype. Canine cytogenetics now presents the biomedical research community with a means to assist in our search for a greater understanding of how genome architectures altered during speciation and in our search for genes associated with cancers that affect both dogs and humans. The cytogenetics ‘toolbox’ for the dog is now loaded. This review aims to provide a summary of some of the recent advancements in canine cytogenetics.

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“…In general, few differences are found between behavioral tests and questionnaire surveys in the consistency of assessing the fearfulness of a dog [ 15 – 17 ]. The genetic background of fear is polygenic and most likely involves genes associated with different cellular processes and pathways, but the specifics remain unclear [ 18 ]. Thus, further studies to identify specific genes involved in the formation and expression of fear are needed.…”

Section: Introductionmentioning

confidence: 99%

Genetic Variants in the STMN1 Transcriptional Regulatory Region Affect Promoter Activity and Fear Behavior in English Springer Spaniels

Xiao-lin

Zhang

et al. 2016

PLoS ONE

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Stathmin 1 (STMN1) is a neuronal growth-associated protein that is involved in microtubule dynamics and plays an important role in synaptic outgrowth and plasticity. Given that STMN1 affects fear behavior, we hypothesized that genetic variations in the STMN1 transcriptional regulatory region affect gene transcription activity and control fear behavior. In this study, two single nucleotide polymorphisms (SNPs), g. -327 and g. -125 , were identified in 317 English Springer Spaniels. A bioinformatics analysis revealed that both were loci located in the canine STMN1 putative promoter region and affected transcription factor binding. A statistical analysis revealed that the genotype at g.-125 produced a significantly greater fear level than that of the genotype (P < 0.05). Furthermore, the H4H4 () haplotype combination was significantly associated with canine fear behavior (P < 0.01). Using serially truncated constructs of the STMN1 promoters and the luciferase reporter, we found that a 395 bp (−312 nt to +83 nt) fragment constituted the core promoter region. The luciferase assay also revealed that the H4 () haplotype promoter had higher activity than that of other haplotypes. Overall, our results suggest that the two SNPs in the canine STMN1 promoter region could affect canine fear behavior by altering STMN1 transcriptional activity.

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Isolation and characterization of the canine serotonin receptor 1B gene (htr1B)

Cited by 12 publications

References 30 publications

Canine Tyrosine Hydroxylase (TH) Gene and Dopamine .BETA.-Hydroxylase (DBH) Gene: Their Sequences, Genetic Polymorphisms, and Diversities among Five Different Dog Breeds

Canine Tyrosine Hydroxylase (TH) Gene and Dopamine .BETA.-Hydroxylase (DBH) Gene: Their Sequences, Genetic Polymorphisms, and Diversities among Five Different Dog Breeds

Canine cytogenetics – from band to basepair

Genetic Variants in the STMN1 Transcriptional Regulatory Region Affect Promoter Activity and Fear Behavior in English Springer Spaniels

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