Molecular cloning and functional characterisation of the galactolipid biosynthetic gene TaMGD in wheat grain

Du, Chenyang; Gao, Honghuan; Liu, Sujun; Ma, Dongyun; Feng, Jianchao; Wang, Chenyang; Jiang, Xiaoxing; Li, Gezi; Xie, Yingxin

doi:10.1016/j.plaphy.2020.04.033

Cited by 2 publications

(2 citation statements)

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“…TaGRF1-2D is a close homolog of OsGRF10 , which is involved in floral organ development in rice ( Liu et al, 2014 ). In addition, we found that the monogalactosyl diacylglycerol (MGD) synthase TaMGD-6B (TraesCS6B02G404700), which controls the number of grains per spike and grain weight per plant in wheat, was bound by Q and upregulated in je0275 ( Du et al, 2020 ). Thus, we concluded that the Q gene regulates a variety of traits, including spike morphology, threshability, heading time, and grain yield, by interacting with these genes.…”

Section: Resultsmentioning

confidence: 99%

“…Interestingly, we found that TaMGD-6B was the target gene of Q and was upregulated in je0275 . Overexpression of TaMGD-6B significantly increased the number of grains per spike and the grain weight per plant but did not influence the 1,000 grain weight in transgenic rice lines ( Du et al, 2020 ). As a previous study also reported that the q allele had higher grain numbers per spike, we speculated that the Q gene affects numerous yield traits by regulating TaMGD-6B ( Xie et al, 2018 ).…”

Section: Discussionmentioning

confidence: 96%

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Identification of the Q Gene Playing a Role in Spike Morphology Variation in Wheat Mutants and Its Regulatory Network

et al. 2022

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The wheat AP2 family gene Q controls domestication traits, including spike morphology and threshability, which are critical for the widespread cultivation and yield improvement of wheat. Although many studies have investigated the molecular mechanisms of the Q gene, its direct target genes, especially those controlling spike morphology, are not clear, and its regulatory pathways are not well established. In this study, we conducted gene mapping of a wheat speltoid spike mutant and found that a new allele of the Q gene with protein truncation played a role in spike morphology variation in the mutant. Dynamic expression levels of the Q gene throughout the spike development process suggested that the transcript abundances of the mutant were decreased at the W6 and W7 scales compared to those of the WT. We identified several mutation sites on the Q gene and showed that mutations in different domains resulted in distinct phenotypes. In addition, we found that the Q gene produced three transcripts via alternative splicing and that they exhibited differential expression patterns in nodes, internodes, flag leaves, and spikes. Finally, we identified several target genes directly downstream of Q, including TaGRF1-2D and TaMGD-6B, and proposed a possible regulatory network. This study uncovered the target genes of Q, and the results can help to clarify the mechanism of wheat spike morphology and thereby improve wheat grain yield.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 96%