Molecular cloning and expression patterns of three putative functional aldehyde oxidase genes and isolation of two aldehyde oxidase pseudogenes in tomato

Min, Xiang Jia; Okada, Kazunori; Brockmann, B.; Koshiba, Tomokazu; Kamiya, Yûji

doi:10.1016/s0167-4781(00)00190-1

Cited by 30 publications

(29 citation statements)

References 14 publications

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“…In addition they both accumulate trans-ABA-alcohol, trans-ABA and catabolites (as does flacca), and are deficient in ABA-aldehyde oxidase activity (Taylor et al, 1988;Duckham et al, 1989;Rock, 1991;Marin and Marion-Poll, 1997;Ori et al, 1997). Although three putative aldehyde oxidase cDNAs have been cloned from tomato (Min et al, 2000), the sitiens gene has not yet been identified and it remains to be determined if sit, dr and aao3 (Seo et al, 2000b) are orthologues. It is unknown why cis and trans isomers of ABA-alcohol (likely reduced from ABA-aldehyde) accumulate in flc and sit mutants, but this phenomenon resulted in discovery of a minor shunt pathway of ABA biosynthesis from ABA-alcohol that bypasses ABA-aldehyde and is catalyzed by a P-450 mono-oxygenase ; Figure 2).…”

Section: Late Specific Steps In Aba Biosynthesismentioning

confidence: 99%

Abscisic Acid Biosynthesis and Response

Finkelstein¹,

Rock

2002

The Arabidopsis Book

190

141

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Section: Late Specific Steps In Aba Biosynthesismentioning

confidence: 99%

Abscisic Acid Biosynthesis and Response

Finkelstein¹,

Rock

2002

The Arabidopsis Book

190

141

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“…Only the sitiens mutant of tomato is thought to have a mutation in a structural gene of an aldehyde oxidase specific for abscisic aldehyde (11,16). Although three putative aldehyde oxidase cDNAs have been cloned from tomato (17,18), the corresponding gene for sitiens has not yet been identified.…”

mentioning

confidence: 99%

The Arabidopsis aldehyde oxidase 3 ( AAO3 ) gene product catalyzes the final step in abscisic acid biosynthesis in leaves

Seo

Peeters

Koiwai

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

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385

310

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Abscisic acid (ABA) is a plant hormone involved in seed development and germination and in responses to various environmental stresses. The last step of ABA biosynthesis involves oxidation of abscisic aldehyde, and aldehyde oxidase (EC 1.2.3.1) is thought to catalyze this reaction. An aldehyde oxidase isoform, AO␦, encoded by AAO3, one of four Arabidopsis aldehyde oxidase genes (AAO1, AAO2, AAO3, and AAO4), is the most likely candidate for the enzyme, because it can efficiently catalyze the oxidation of abscisic aldehyde to ABA. Here, we report the isolation and characterization of an ABA-deficient Arabidopsis mutant that maps at the AAO3 locus. The mutant exhibits a wilty phenotype in rosette leaves, but seed dormancy is not affected. ABA levels were significantly reduced in the mutant leaves, explaining the wilty phenotype in rosettes, whereas the level in the mutant seeds was less reduced. No AO␦ activity could be detected in the rosette leaves of the mutant. Sequence data showed that the mutant contains a G to A substitution in the AAO3 gene. The mutation causes incorrect splicing of the ninth intron of AAO3 mRNA. We thus conclude that the ABA-deficient mutant is impaired in the AAO3 gene and that the gene product, AO␦, is an aldehyde oxidase that catalyzes the last step of ABA biosynthesis in Arabidopsis, specifically in rosette leaves. Other aldehyde oxidases may be involved in ABA biosynthesis in other organs.

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“…The tomato ABA-deficient mutant sitiens is thought to be defective in one of the tomato aldehyde oxidase (TAO) genes (Taylor et al 1988, Marin and Marion-Poll 1997, Min et al 2000. The extent of ABA deficiency in sitiens is similar to that in another mutant, flacca, which has a defect in MOSU (Taylor and Tarr 1984).…”

Section: Discussionmentioning

confidence: 99%

Comparative Studies on the Arabidopsis Aldehyde Oxidase (AAO) Gene Family Revealed a Major Role of AAO3 in ABA Biosynthesis in Seeds

Seo

Aoki

Koiwai

et al. 2004

Plant and Cell Physiology

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170

124

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;The Arabidopsis aldehyde oxidase 3 (AAO3) gene encodes an enzyme that catalyzes the final step of ABA biosynthesis. AAO3 has been shown to be the major AAO involved in ABA biosynthesis in leaves under stress conditions. On the other hand, less severe phenotypes of the aao3 seeds suggested that other AAO(s) might also be involved in ABA biosynthesis in seeds. Among four AAOs (AAO1-AAO4), AAO1 and AAO4 were the AAO expressed most abundantly in dry seeds and developing siliques, respectively. Unlike aao3, single loss-of-function mutants for AAO1 and AAO4 (aao1 and aao4), failed to show significant changes in endogenous ABA levels in seeds when compared with wild type. While aao3 seed germination was resistant to the gibberellin biosynthesis inhibitor, uniconazole, aao1 and aao4 showed no resistance and were similar to wild type. These results indicate that AAO3, but not AAO1 or AAO4, plays an important role in ABA biosynthesis in seeds. Mutations of AAO1 or AAO4 in the aao3 mutant background enhanced ABA deficiency in seeds, demonstrating that both gene products contribute partially to ABA biosynthesis in the aao3 mutant background. However, considering the enzymatic characters of AAO1 and AAO4, their involvement in ABA biosynthesis in wild-type seeds may be negligible. We have concluded that AAO3 is the AAO that plays a major role in ABA biosynthesis in Arabidopsis seeds as well as in leaves.

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Molecular cloning and expression patterns of three putative functional aldehyde oxidase genes and isolation of two aldehyde oxidase pseudogenes in tomato

Cited by 30 publications

References 14 publications

Abscisic Acid Biosynthesis and Response

Abscisic Acid Biosynthesis and Response

The Arabidopsis aldehyde oxidase 3 ( AAO3 ) gene product catalyzes the final step in abscisic acid biosynthesis in leaves

Comparative Studies on the Arabidopsis Aldehyde Oxidase (AAO) Gene Family Revealed a Major Role of AAO3 in ABA Biosynthesis in Seeds

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