Molecular cloning and expression of MyD88 in large yellow croaker, Pseudosciaena crocea

Functional genomic studies on an immune- and antiviral-related gene of MyD88 in orange-spotted grouper, Epinephelus coioides

“…Og-MyD88 showed higher expression levels in immune response-related tissues, including spleen, head kidney, kidney, skin and intestine. The tissue distribution pattern of Og-MyD88 is similar to MyD88 in other species, including mammalian [18], avian [20], amphibian [21] and other fish [28,30,31].…”

“…Following this, the death domain of MyD88 mediates the interaction with the corresponding domain in IL-1R-associated kinases (IRAKs), leading to recruitment of downstream immune molecules and resulting in activation of NF-κB, IRFs and induction of proinflammatory cytokines or antiviral genes [10,24,25]. Until now, MyD88 orthologs have been identified in several fish species, including zebrafish [26], rainbow trout [27], Japanese flounder [28], Atlantic salmon [29], large yellow croaker and rock bream [30,31]. However, the roles of MyD88 in fish innate immune responses, especially in combating virus infection, have not been fully elucidated.…”

Effects of dietary size-fractionated fish hydrolysates on growth, activities of digestive enzymes and aminotransferases and expression of some protein metabolism related genes in large yellow croaker (Larimichthys crocea) larvae

“…The absolute value of the slope was less than 0.1, which was close to zero and indicated that ΔΔC T calculation for the relative quantification of target genes could be use. To calculate the expression of PepT1, CCK, trypsin and TOR, the comparative CT method (2 − ΔΔCt method) was used as described by Yao et al (2009).…”

Characterization, molecular cloning, and expression analysis of Ecsit in the spinyhead croaker, Collichthys lucidus

“…In recent years, many TLRs and MYD88 proteins (important adaptor proteins involved in the TLR signaling pathway) have been studied. For instance, MYD88 (Yao et al, 2009), TLR1 , TLR2 (Baoprasertkul et al, 2007a), TLR3 (Baoprasertkul et al, 2006), TLR4 (Jault et al, 2004), TLR5 (Baoprasertkul et al, 2007b), TLR7 and TLR8 , TLR9 (Franch et al, 2006), TLR20 and TLR21 (Baoprasertkul et al, 2007b;Rebl et al, 2007) have been identified and characterized in fishes. However, only a few Ecsits have so far been identified in species other than mammals.…”