Molecular cloning and expression of a cDNA encoding 1-deoxy-d-xylulose-5-phosphate synthase from oil palm Elaeis guineensis Jacq.

Khemvong, Sawitri; Suvachittanont, Wallie

doi:10.1016/j.plantsci.2005.05.001

Cited by 42 publications

(21 citation statements)

References 48 publications

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“…Our results in C. stellatopilosus are in agreement with studies in Coleus forskohlii, 20) Elaeis guineensis 21) and Camptotheca acuminata. 22) Those studies concluded that the MEPS protein is not a rate limiting step in the DXP pathway, however, it plays an important role in the regulation of terpenoid production.…”

Section: Resultssupporting

confidence: 92%

Transcription Profiles Analysis of Genes Encoding 1-Deoxy-D-xylulose 5-Phosphate Synthase and 2C-Methyl-D-erythritol 4-Phosphate Synthase in Plaunotol Biosynthesis from Croton stellatopilosus

Wungsintaweekul

Sirisuntipong

Kongduang

et al. 2008

Biological & Pharmaceutical Bulletin

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The deoxyxylulose phosphate (DXP) pathway was completely elucidated in 2002 as the alternative pathway of isoprenoid biosynthesis in Escherichia coli.1) Distribution in nature of the DXP pathway occurs in bacteria, malaria parasites and higher plants.2) The latter, compartment-dependent isoprenoid biosynthesis was firstly observed in Ginkgo biloba. 3)Schwarz and Arigoni suggested that monoterpenoids, diterpenoids, tetraterpenoids are biosynthesized from the DXP pathway in plastids, whereas triterpenoids and sterols derive from the classical mevalonate (MVA) pathway in the cytoplasm.3) However, the contributions of both pathways between the two compartments have been shown to different according to plant species and type of cultures.2) Many studies have since re-investigated the genuine source of isoprene units in order to better understand the biosynthesis of terpenoid compounds.Croton stellatopilosus OHBA (formerly named C. sublyratus KURZ., Euphorbiaceae) 4) contains the mucosal protective factor-enhancing antiulcer agent, plaunotol.5) Plaunotol has therapeutic effects for peptic ulcers including Helicobacter pylori-associated and non-steroidal anti-inflammatory drug induced ulcers. 6) Biosynthetically, plaunotol is formed from geranylgeranyl diphosphate, a general precursor of diterpene compounds. Geranylgeranyl diphosphate (GGPP) phosphatase then cleaves the phosphate group from GGPP yielding geranylgeraniol (GGOH).7) Finally, GGOH is hydroxylated at C-18 by geranylgeraniol 18-hydroxylase, affording plaunotol ( Fig. 1). 8)We have previously reported a study on the biosynthesis of plaunotol, an acyclic diterpene alcohol, in C. stellatopilosus young shoots. The results suggested that four isoprene units in the skeleton of plaunotol are exclusively supplied from the DXP pathway.9) Earlier studies on the phytochemicals and their localization demonstrated that production of intermediates for terpenoid biosynthesis was related to chloroplastforming cells. 10,11) However for the early steps of isoprenoid biosynthesis in C. stellatopilosus, there is lack of knowledge about genes and enzymes, which have not as yet been reported. Therefore, the objective of this work is to study the genes encoding the 1-deoxy-D-xylulose 5-phosphate synthase (CSDXS) and 2C-methyl-D-erythritol 4-phosphate synthase (CSMEPS) from C. stellatopilosus. Expression profiling of the CSDXS and the CSMEPS in leaves, twigs and roots were investigated in terms of the levels of mRNA expression and plaunotol accumulation. The results obtained from this study will be useful for understanding the plaunotol biosynthetic pathway, which may lead to enhance plaunotol production either in intact plants or plant cell cultures by metabolic engineering. MATERIALS AND METHODS Plant MaterialsYoung leaves of C. stellatopilosus were collected from a two-year old plant, which grows in the botanical garden of the Faculty of Pharmaceutical Sciences, Prince of Songkla University (PSU), Hat Yai Campus, Songkhla, Thailand. A voucher specimen was deposited in the Souther...

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Section: Resultssupporting

confidence: 92%

Transcription Profiles Analysis of Genes Encoding 1-Deoxy-D-xylulose 5-Phosphate Synthase and 2C-Methyl-D-erythritol 4-Phosphate Synthase in Plaunotol Biosynthesis from Croton stellatopilosus

Wungsintaweekul

Sirisuntipong

Kongduang

et al. 2008

Biological & Pharmaceutical Bulletin

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“…The initial step of the MEP pathway involves the condensation of pyruvate and glyceraldehyde-3-phosphate which results in 1-deoxy-D-xylulose-5-phosphate (DXP). 1-deoxy-D-xylulose-5-phosphate synthase (DXS) is the first enzyme that catalyzes this transketolase reaction (Khemvong and Suvachittanont 2005) (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

Increasing anthraquinone production by overexpression of 1-deoxy-d-xylulose-5-phosphate synthase in transgenic cell suspension cultures of Morinda citrifolia

et al. 2010

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A Morinda citrifolia cell line was obtained by overexpresion of 1-deoxy-D: -xylulose 5-phosphate synthase (DXS) from Catharanthus roseus, a key enzyme of the metabolic pathway of anthraquinones (AQs). This cell line increased AQs production by about 24% compared to the control cell line. This transgenic cell line which carries dxs cDNA isolated from Catharanthus roseus, was achieved by direct transformation of cell suspension cultures of M. citrifolia using a hypervirulent Agrobacterium tumefaciens strain. The effects of the overexpression of the dxs gene also resulted in increased levels of dxs mRNA transcripts and DXS activity compared to the control cell line. In addition, total phenolics and phenylalanine ammonia-lyase activity were evaluated and were significantly higher in the transgenic line than in controls.

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“…Despite the attractive quantitative and qualitative carotenoid composition of the oil palm mesocarp, very few studies have focused on the transcriptional mechanisms underlying carotenoid gene metabolism or performed metabolite profiling during mesocarp development (Ikemefuna and Adamson, 1984;Khemvong and Suvachittanont, 2005;Rasid et al, 2008). Only two genes, PHYTOENE SYNTHASE (PSY) and 1-DEOXY-XYLULOSE 5-PHOSPHATE SYNTHASE (DXS), have been investigated to date in oil palm.…”

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confidence: 99%

Regulatory Mechanisms Underlying Oil Palm Fruit Mesocarp Maturation, Ripening, and Functional Specialization in Lipid and Carotenoid Metabolism

Tranbarger

Dussert²,

Joët³

et al. 2011

Plant Physiology

194

220

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Fruit provide essential nutrients and vitamins for the human diet. Not only is the lipid-rich fleshy mesocarp tissue of the oil palm (Elaeis guineensis) fruit the main source of edible oil for the world, but it is also the richest dietary source of provitamin A. This study examines the transcriptional basis of these two outstanding metabolic characters in the oil palm mesocarp. Morphological, cellular, biochemical, and hormonal features defined key phases of mesocarp development. A 454 pyrosequencingderived transcriptome was then assembled for the developmental phases preceding and during maturation and ripening, when high rates of lipid and carotenoid biosynthesis occur. A total of 2,629 contigs with differential representation revealed coordination of metabolic and regulatory components. Further analysis focused on the fatty acid and triacylglycerol assembly pathways and during carotenogenesis. Notably, a contig similar to the Arabidopsis (Arabidopsis thaliana) seed oil transcription factor WRINKLED1 was identified with a transcript profile coordinated with those of several fatty acid biosynthetic genes and the high rates of lipid accumulation, suggesting some common regulatory features between seeds and fruits. We also focused on transcriptional regulatory networks of the fruit, in particular those related to ethylene transcriptional and GLOBOSA/PISTILLATA-like proteins in the mesocarp and a central role for ethylene-coordinated transcriptional regulation of type VII ethylene response factors during ripening. Our results suggest that divergence has occurred in the regulatory components in this monocot fruit compared with those identified in the dicot tomato (Solanum lycopersicum) fleshy fruit model.

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Molecular cloning and expression of a cDNA encoding 1-deoxy-d-xylulose-5-phosphate synthase from oil palm Elaeis guineensis Jacq.

Cited by 42 publications

References 48 publications

Transcription Profiles Analysis of Genes Encoding 1-Deoxy-D-xylulose 5-Phosphate Synthase and 2C-Methyl-D-erythritol 4-Phosphate Synthase in Plaunotol Biosynthesis from Croton stellatopilosus

Transcription Profiles Analysis of Genes Encoding 1-Deoxy-D-xylulose 5-Phosphate Synthase and 2C-Methyl-D-erythritol 4-Phosphate Synthase in Plaunotol Biosynthesis from Croton stellatopilosus

Increasing anthraquinone production by overexpression of 1-deoxy-d-xylulose-5-phosphate synthase in transgenic cell suspension cultures of Morinda citrifolia

Regulatory Mechanisms Underlying Oil Palm Fruit Mesocarp Maturation, Ripening, and Functional Specialization in Lipid and Carotenoid Metabolism

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