Increasing anthraquinone production by overexpression of 1-deoxy-d-xylulose-5-phosphate synthase in transgenic cell suspension cultures of Morinda citrifolia

Quevedo, Carla; Perassolo, Marı́a; Alechine, Evguenia; Corach, Daniel; Giulietti, Ana María; Talou, Julián Rodríguez

doi:10.1007/s10529-010-0250-8

Cited by 14 publications

(5 citation statements)

References 22 publications

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“…It was found that over-expression of AtDXS in Daucus carota resulted in remarkably higher accumulation of both carotenoids and chlorophylls (Simpson et al 2016). Similar results were also obtained in T. wilfordii (Zhang et al 2019), C. roseus (Peebles et al 2011), and other plants (Augustin et al 2015;Kai et al 2011;Morris et al 2006;Muñoz-Bertomeu et al 2006;Quevedo et al 2010;Vaccaro et al 2019). In addition, due to the bacteriostatic and bactericidal effects of terpenoids (Gershenzon and Dudareva 2007), the increase of terpenoid content may help plants resist to pathogens.…”

Section: Over-expression and Co-expression Regulation Of Dxs In Plantssupporting

confidence: 55%

A systematic review of 1-Deoxy-D-xylulose-5-phosphate synthase in terpenoid biosynthesis in plants

et al. 2021

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1-Deoxy-D-xylulose-5-phosphate synthase (DXS) is the first rate-limiting enzyme involved in the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for terpenoids biosynthesis. Up to date, three types of DXS (DXS1, DXS2, and DXS3) have been identified in plants. In this review, we searched all the plant DXS orthologs currently registered in the NCBI database, and selected 40 of them for phylogenetic, physicochemical properties, and structure analyses. Our bioinformatic analysis indicates that three types of DXS are all stable and conservative and that the evolutionary discrimination among them is very clear. We provide a summary of our current understanding about expression and regulation of DXS based on reports published in the past two decades, which demonstrates that DXS plays essential roles in many physiological processes, such as photosynthesis, phytohormone regulation, adversity resistance, and pathogen defense. However, additional studies are still needed for a better understanding of the molecular mechanisms underlying the roles of DXS in these biological processes. Moreover, clarifying the X-ray diffraction structure of DXS from plants remains a major issue to be addressed. This review will lay a foundation for further studies of DXS in plants.

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Section: Over-expression and Co-expression Regulation Of Dxs In Plantssupporting

confidence: 55%

A systematic review of 1-Deoxy-D-xylulose-5-phosphate synthase in terpenoid biosynthesis in plants

et al. 2021

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“…In contrast, the most highly expressed enzyme in the MEP pathway was the terminal enzyme HDR (1-hydroxy-2-methyl-butenyl 4-diphosphate reductase), which catalyzes the formation of isopentenyl diphosphate (IPP) and its isomer, dimethylallyl diphosphate (DMAPP). Anthraquinone production is increased by the overexpression of 1-deoxy-D: -xylulose-5-phosphate synthase (DXS) in Morinda citrifolia cells [44]. In Polygonum, DXS had the lowest expression (RPKM 12.2) compared to the genes coding the downstream enzyme ( Figure 4B).…”

Section: Genes Involved In Secondary Metabolismmentioning

confidence: 99%

De novo characterization of the root transcriptome of a traditional Chinese medicinal plant Polygonum cuspidatum

Hao

Ma²,

et al. 2012

Sci. China Life Sci.

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Various active components have been extracted from the root of Polygonum cuspidatum. However, the genetic basis for their activity is virtually unknown. In this study, 25600002 short reads (2.3 Gb) of P. cuspidatum root transcriptome were obtained via Illumina HiSeq 2000 sequencing. A total of 86418 unigenes were assembled de novo and annotated. Twelve, 18, 60 and 54 unigenes were respectively mapped to the mevalonic acid (MVA), methyl-D-erythritol 4-phosphate (MEP), shikimate and resveratrol biosynthesis pathways, suggesting that they are involved in the biosynthesis of pharmaceutically important anthraquinone and resveratrol. Eighteen potential UDP-glycosyltransferase unigenes were identified as the candidates most likely to be involved in the biosynthesis of glycosides of secondary metabolites. Identification of relevant genes could be important in eventually increasing the yields of the medicinally useful constituents of the P. cuspidatum root. From the previously published transcriptome data of 19 non-model plant taxa, 1127 shared orthologs were identified and characterized. This information will be very useful for future functional, phylogenetic and evolutionary studies of these plants.

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“…It has been reported that the overexpression of DXS in Arabidopsis led to an increase in terpenoid concentrations (Estévez et al, 2001). Overexpression of DXS in transgenic cell-suspension cultures of Morinda citrifolia increases anthraquinone production (Quevedo et al, 2010). These results suggest that the overexpression of DXS can increase secondary metabolic concentrations; however, cloning and analysis of the DXS gene are important prerequisites.…”

Section: Discussionmentioning

confidence: 98%

“…In Catharanthus roseus hairy roots, the expression of DXS and geraniol-10-hydroxylase or anthranilate synthase increases accumulation of the terpenoid indole alkaloid (Peebles et al, 2011). In addition, overexpression of DXS increases anthraquinone production in transgenic cell-suspension cultures of Morinda citrifolia (Quevedo et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Cloning and expression analysis of 1-deoxy-D-xylulose-5-phosphate synthase gene from the medicinal plant Conyza blinii H.Lév.

Sun¹,

Liu²,

Gao³

et al. 2014

Turk J Biol

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Conyza blinii H.Lév. is a traditional Chinese medicinal plant that is distributed mainly in southwestern Sichuan and northern Yunnan. Its characteristic product is blinin, which has, among other properties, antigastric ulcer activity, and can serve as a qualitycontrol standard for such medicine. The problem is that C. blinii only produces low yields of blinin. As a diterpene, blinin is likely formed by the methylerythritol phosphate pathway. While 1-deoxy-D-xylulose-5-phosphate synthase (DXS) is the first rate-limiting enzyme in diterpenoid biosynthesis, it is a switch in the pathway. The DXS gene was successfully cloned and characterized from C. blinii by homologous cloning and rapid-amplification of cDNA ends (RACE). It was designated cbDXS and contains a 2190-bp open reading frame encoding 730 amino acids (aa), including a 17-aa signal peptide and a 713-aa mature protein. Semiquantitative RT-PCR was used to determine the expression levels of cbDXS in different C. blinii tissues at the seedling stage. The corresponding blinin concentrations were also analyzed by high-performance liquid chromatography (HPLC). The cbDXS gene showed tissue specificity. Moreover, its expression levels were highly correlated to blinin concentrations. In summary, it is suggested that overexpression of this gene may increase flux toward blinin synthesis.

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Increasing anthraquinone production by overexpression of 1-deoxy-d-xylulose-5-phosphate synthase in transgenic cell suspension cultures of Morinda citrifolia

Cited by 14 publications

References 22 publications

A systematic review of 1-Deoxy-D-xylulose-5-phosphate synthase in terpenoid biosynthesis in plants

A systematic review of 1-Deoxy-D-xylulose-5-phosphate synthase in terpenoid biosynthesis in plants

De novo characterization of the root transcriptome of a traditional Chinese medicinal plant Polygonum cuspidatum

Cloning and expression analysis of 1-deoxy-D-xylulose-5-phosphate synthase gene from the medicinal plant Conyza blinii H.Lév.

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