Molecular cloning and expression of bovine interleukin-8

Morsey, Mohamad A.; Popowych, Yurij; Kowalski, Jacek; Gerlach, Gerald-F.; Godson, Dale L.; Campos, Manuel; Babiuk, Lorne A.

doi:10.1006/mpat.1996.0019

Cited by 42 publications

(24 citation statements)

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“…In addition, IL-8 gene expression can be detected in tissue macrophages, such as mammalian (porcine and bovine) alveolar macrophages [37,38]. Recently, constitutive expression of IL-8 gene has also been reported in fish, such as Atlantic cod [18], haddock [17], rainbow trout [15], carp [39] and channel catfish [16].…”

Section: Discussionmentioning

confidence: 99%

“…These findings indicate that after an early expression of IL-8 mRNA in the lymphoid organ, turtle liver may be also involved in the significant IL-8 induction. It has been reported that the large increases in IL-8 mRNA could be detected in some mammalian alveolar macrophages and chicken spleen macrophages after stimulation with heat killed bacteria and/or LPS [37,38] and with infectious bursal disease virus [42], respectively. Therefore, whether the upregulated expression of IL-8 mRNA in the liver of infected turtles is related to the Kupffer cell (the macrophages in hepatic sinusoid) activation needs to be investigated further.…”

Section: Discussionmentioning

confidence: 99%

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Molecular characterization and expression profiles in response to bacterial infection of Chinese soft-shelled turtle interleukin-8 (IL-8), the first reptilian chemokine gene

Zhou

Guo

Dai

2009

Developmental & Comparative Immunology

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Molecular characterization and expression profiles in response to bacterial infection of Chinese soft-shelled turtle interleukin-8 (IL-8), the first reptilian chemokine gene

Zhou

Guo

Dai

2009

Developmental & Comparative Immunology

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“…In humans, CXC chemokines have four highly conserved cysteine residues and are characterized by a single amino acid between the number 1 and 2 cysteines as a CXC motif [35]. Ferret IL-8 has four cysteine residues, the same as other mammals, including humans [21,26]. Ferret TNF-α had two cysteine residues at Cys 145 and 177 .…”

Section: Discussionmentioning

confidence: 99%

“…Glu 21 and Val 77 and comprised 147, 235, 183, 81 and 157 amino acids, respectively. Cysteine residues were conserved at Cys 23 in ferret IFN-γ protein (Fig.…”

Section: The Multi-alignments and Characterization Of The Deduced Amimentioning

confidence: 99%

Molecular Cloning and Phylogenetic Analysis of Inflammatory Cytokines of the Ferret (Mustela putorius furo)

Nakata

Itou

Sakai

2008

The Journal of Veterinary Medical Science

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ABSTRACT. The present study determined the cDNA and deduced amino acid sequences of ferret (Mustela putorius furo) inflammatory cytokines, interferon (IFN)-γ, interleukin (IL)-1β, IL-6, IL-8 and tumor necrosis factor (TNF)-α. The homologies of the nucleotide sequences of IFN-γ, IL-1β, IL-6, IL-8 and TNF-α of the ferret to those from other mammalian species ranged from 64.3-92.9%, 73.0-83.9, 58.1-84.8%, 58.1-89.7% and 79.0-95.0%, respectively. As distinctive amino acid residues constituting various motifs and ligandbinding sites and cysteine residues were highly conserved in ferret inflammatory cytokine proteins, ferret cytokines may have fundamentally similar functions to those of other mammals. Phylogenetic analyses based on the deduced amino acid sequences revealed that all ferret inflammatory cytokines were more closely related to those of the Carnivora order, specifically dog and cat, than to other species.

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“…11) The EC secrete IL-8, a neutrophil-activating cytokine, [12][13][14] that acts as a mediator of inflammation, 15) reduces chemotactic activity, 16) and elicits leukocyte extravasation. 14,17) The hematopoietic cytokine, GM-CSF, increases erythroid and multipotential colony formation in enriched human bone marrow and peripheral blood progenitor cell cultures containing erythropoietin, [18][19][20][21] and is both an effective stimulator of leukocytosis 22) and an activator of the phagocytic function of mature neutrophils in vivo.…”

mentioning

confidence: 99%

Release of Cytokines from Human Umbilical Vein Endothelial Cells Treated with Platinum Compounds in vitro

Shi¹,

Inoue²,

Shinozaki³

et al. 1998

Japanese Journal of Cancer Research

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Endothelial cells (EC) produce cytokines, such as interleukin (IL)-1, IL-6, IL-8 and granulocytemacrophage colony-stimulating factor (GM-CSF). These cytokines have an important role in the proliferation and differentiation of hematopoietic progenitor cells. On the other hand, anticancer agents generally cause hematopoietic disorders. However, little is known about the effects of chemotherapeutic agents on the secretion of cytokines from EC. Therefore, we investigated if treatment with platinum compounds may stimulate EC to secrete cytokines. EC newly isolated from a human umbilical vein were exposed to cisplatin, carboplatin, or TRK-710 for 80 min, then the cells were washed and placed in fresh medium. The levels of cytokines in the fresh medium were measured by the ELISA method, the levels of intracellular hydrogen peroxide (H 2 O 2 ) were measured by flow cytometry, and the rhodamine 123-stained live mitochondria of the EC were observed under a confocal laser microscope. Platinum compounds induced cytokine production in human EC: cisplatin most prominently induced the release of IL-1 and IL-6, and TRK-710 had the greatest ability to induce the release of GM-CSF. Intracellular H 2 O 2 production and IL-8 release were transiently induced immediately after treatment with platinum compounds, leading to IL-1 release when H 2 O 2 production was eliminated. These results may provide new insights into the hematological toxicity induced by anticancer agents and the role of IL-1 and IL-6 secreted from EC in this toxicity.

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Molecular cloning and expression of bovine interleukin-8

Cited by 42 publications

References 0 publications

Molecular characterization and expression profiles in response to bacterial infection of Chinese soft-shelled turtle interleukin-8 (IL-8), the first reptilian chemokine gene

Molecular characterization and expression profiles in response to bacterial infection of Chinese soft-shelled turtle interleukin-8 (IL-8), the first reptilian chemokine gene

Molecular Cloning and Phylogenetic Analysis of Inflammatory Cytokines of the Ferret (Mustela putorius furo)

Release of Cytokines from Human Umbilical Vein Endothelial Cells Treated with Platinum Compounds in vitro

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