Molecular cloning and expression of interleukin-1 receptor-associated kinase 4, an important mediator of Toll-like receptor signal pathway, from small abalone Haliotis diversicolor

Ge, Hui; Wang, Guodong; Zhang, Lili; Zhang, Ziping; Wang, Shuhong; Zou, Zhihua; Su-fen, Yan; Wang, Yilei

doi:10.1016/j.fsi.2011.02.018

Cited by 31 publications

(16 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…High expression of csIRAK-4 in unfertilized eggs suggested the existence of maternal mRNAs. And the presence of maternal transcripts of IRAK-4 was also reported in zebrafish and abalone [3,10]. Besides IRAK-4, we also detected the maternal mRNAs of other immune-related genes (TLR9, MyD88 and MHC) in C. semilaevis.…”

Section: Developmental Expression Of the Csirak-4supporting

confidence: 68%

“…Similarly, IRAK-4 has also been detected in various tissues of other fish and human [10,18]. The constitutive expression of IRAK-4 indicated it might be involved in a wide variety of physiological processes [3]. In teleosts, spleen and head kidney play essential roles in the immune defense, just like bone marrow and lymph nodes in mammals [19,20].…”

Section: Tissue Mrna Expression Of the Csirak-4mentioning

confidence: 99%

“…Up to now, four members of IRAK family (IRAK-1, 2, M, and 4) have been described [3]. Although all IRAKs are categorized as serine/threonine protein kinases and share similar domains, they have distinct functional roles in signal transduction [4].…”

Section: Introductionmentioning

confidence: 99%

“…However, to our knowledge, indepth expression analysis and characterization of IRAK-4 gene have only been conducted in zebrafish (Danio rerio) [10] and small abalone (Haliotis diversicolor) [3]. Although these identified IRAK-4s shared high identity in amino acid sequence, diverse responses of IRAK-4 to experimental challenges were observed in different species [10e12].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Identification and characterization of IL-1 receptor-associated kinase-4 (IRAK-4) in half-smooth tongue sole Cynoglossus semilaevis

Zhong

et al. 2012

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Section: Developmental Expression Of the Csirak-4supporting

confidence: 68%

Section: Tissue Mrna Expression Of the Csirak-4mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification and characterization of IL-1 receptor-associated kinase-4 (IRAK-4) in half-smooth tongue sole Cynoglossus semilaevis

Zhong

et al. 2012

Fish & Shellfish Immunology

View full text Add to dashboard Cite

“…Similar tissue-distribution pattern was also found in other species. Some immunity-related genes identified from abalone, such as saIRAK-4, had the highest expression in the gills (Ge et al, 2011). Allograft inflammatory factory-1 (AIF-1) of sea cucumber was highly expressed in the tissues of respiratory tree, tube, body wall and CM (Ji et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Isolation of immune-relating 185/333-1 gene from Sea Urchin (Strongylocentrotus intermedius) and Its expression analysis

Wang

Ding

Liu

et al. 2016

J. Ocean Univ. China

View full text Add to dashboard Cite

The 185/333 gene family involved in the immune response of sea urchin. One 185/333 cDNA was isolated from Strongylocentrotus intermedius, and named as Si185/333-1. Its full-length cDNA was 1246 bp in length with a 906 bp open reading frame encoding a protein of 301 aa. The molecular weight of the deduced protein was approximately 33.1 kD with an estimated PI of pH 6.26. Si185/333-1 had high identities (70%-86%) to most of Sp185/333. An extraordinary identity of 92% was found between Si185/333-1 and Sp185/333 C5 alpha (ABR22474). Moderate identities (63%-64%) were displayed between Si185/333-1 and He185/333. Si185/333-1 had similar structure to Sp185/333. A signal-peptide, a gly-rich region and a his-rich region were found in its secondary structure. RGD motif was found in gly-rich region at position 116-118aa. There was no transmembrane region in Si185/333-1. The element pattern of Si185/333-1 is different from any available pattern that identified in Sp185/333. Si185/333-1 clustered together with pattern C Sp185/333 in phylogenetic tree. The Si185/333-1 mRNA could be detected in tissues including peristomial membrane, coelomocytes, muscle of Aristotles lantern, gut and tube feet, with the highest expression level detected in peristomial membrane and a relatively low expression in ovary and testis. The temporal expression of Si185/333-1 in peristomial membrane and coelomocytes were up-regulated after bacterial, β-D-glucan and dsRNA challenges, reaching the maximum at 12 h post-stimulation. The up-regulation was more obvious in coelomocytes, and bacterial challenge triggered the highest response. These results proved that 185/333-1 gene was involved in the immune defense of S. intermedius, while more studies were necessary for its function in S. intermedius immunity.

show abstract

Disease and potential disease agents in wild and cultured abalone

Moore

2023

Abalone: Biology, Ecology, Aquaculture and Fisheries

View full text Add to dashboard Cite

Molecular cloning and expression of interleukin-1 receptor-associated kinase 4, an important mediator of Toll-like receptor signal pathway, from small abalone Haliotis diversicolor

Cited by 31 publications

References 45 publications

Identification and characterization of IL-1 receptor-associated kinase-4 (IRAK-4) in half-smooth tongue sole Cynoglossus semilaevis

Identification and characterization of IL-1 receptor-associated kinase-4 (IRAK-4) in half-smooth tongue sole Cynoglossus semilaevis

Isolation of immune-relating 185/333-1 gene from Sea Urchin (Strongylocentrotus intermedius) and Its expression analysis

Disease and potential disease agents in wild and cultured abalone

Contact Info

Product

Resources

About