Identification and characterization of IL-1 receptor-associated kinase-4 (IRAK-4) in half-smooth tongue sole Cynoglossus semilaevis

Yu, Yan; Zhong, Qing; Li, Chunmei; Jiang, Liming; Wang, Yanan; Sun, Yeying; Wang, Xubo; Wang, Zhigang; Zhang, Quanqi

doi:10.1016/j.fsi.2011.12.011

Cited by 22 publications

(7 citation statements)

References 31 publications

(41 reference statements)

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“…Our candidate factors contributing downstream to tlr signaling were eventually upregulated in the livers of infected fish and none of them was significantly downregulated at any time after infection. Upregulated mRNA concentrations of such factors in liver were reported from a variety of challenge studies and infection experiments in different fish species [56][57][58][59]. Irak1 was distinct from all those other factors in so far as its mRNA abundance was increased already at 6 hpi, not only in liver, but also in spleen and head kidney.…”

Section: Irak1 May Have a Peculiar Role For Constraining Tlr Signalinmentioning

confidence: 90%

Aeromonas salmonicidaInfection Only Moderately Regulates Expression of Factors Contributing to Toll-Like Receptor Signaling but Massively Activates the Cellular and Humoral Branches of Innate Immunity in Rainbow Trout (Oncorhynchus mykiss)

Brietzke

Korytář

Jaros

et al. 2015

Journal of Immunology Research

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Toll-like receptors (TLRs) are known to detect a defined spectrum of microbial structures. However, the knowledge about the specificity of teleost Tlr factors for distinct pathogens is limited so far. We measured baseline expression profiles of 18 tlr genes and associated signaling factors in four immune-relevant tissues of rainbow trout Oncorhynchus mykiss. Intraperitoneal injection of a lethal dose of Aeromonas salmonicida subsp. salmonicida induced highly increased levels of cytokine mRNAs during a 72-hour postinfection (hpi) period. In contrast, only the fish-specific tlr22a2 and the downstream factor irak1 featured clearly increased transcript levels, while the mRNA concentrations of many other tlr genes decreased. Flow cytometry quantified cell trafficking after infection indicating a dramatic influx of myeloid cells into the peritoneum and a belated low level immigration of lymphoid cells. T and B lymphocytes were differentiated with RT-qPCR revealing that B lymphocytes emigrated from and T lymphocytes immigrated into head kidney. In conclusion, no specific TLR can be singled out as a dominant receptor for A. salmonicida. The recruitment of cellular factors of innate immunity rather than induced expression of pathogen receptors is hence of key importance for mounting a first immune defense against invading A. salmonicida.

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Section: Irak1 May Have a Peculiar Role For Constraining Tlr Signalinmentioning

confidence: 90%

Aeromonas salmonicidaInfection Only Moderately Regulates Expression of Factors Contributing to Toll-Like Receptor Signaling but Massively Activates the Cellular and Humoral Branches of Innate Immunity in Rainbow Trout (Oncorhynchus mykiss)

Brietzke

Korytář

Jaros

et al. 2015

Journal of Immunology Research

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“…Consistently, the omnipresence of OfIRAK4 transcripts implied its involvement in a broad spectrum of physiological processes [10,40]. Earlier reports revealed the teleostean IRAK4 to be dominantly expressed in head kidney and spleen [16,17,19]. Nevertheless, similar to a contrasting pattern noticed in roughskin sculpin with highest IRAK4 transcripts in skin [18], OfIRAK4 mRNA level was prominently detected in liver, along with moderate levels in head kidney and spleen.…”

Section: Discussionmentioning

confidence: 55%

“…A few invertebrate IRAK4 homologs have been identified, including those from Euprymna scolopes [12], Suberites domuncula [13], Mya arenaria [14], Haliotis diversicolor [15], and their mRNA expression has been investigated in healthy tissues and postpathogen infection. To the best of our knowledge, a few teleostean IRAK4 homologs have also been reported from Danio rerio [16], Cynoglossus semilaevis [17], Trachidermus fasciatus [18] and three salmonids [19]. However, these studies have not provided an insight into the exon-intron structure (except the recently published data in salmonids [19]), and genomic evolution of IRAK4 in teleost with respect to other lineages.…”

Section: Introductionmentioning

confidence: 94%

Insights into molecular profiles and genomic evolution of an IRAK4 homolog from rock bream (Oplegnathus fasciatus): Immunogen- and pathogen-induced transcriptional expression

Umasuthan¹,

Bathige²,

Whang

et al. 2015

Fish & Shellfish Immunology

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“…Because the harvested ovary mainly consisted of unspawned eggs, this observation suggests that BbIRAK4, but not BbPelle, is abundant as maternal transcripts and may have an important role in embryogenesis. In line with this, abundant maternal transcripts of IRAK4 were found in half-smooth tongue sole, zebrafish, and abalone (26,27,67). Moreover, the Drosophila Tube, the homolog of IRAK4, has been shown to participate in the formation of the dorsoventral axis during development (66).…”

Section: Expression Profile Of Amphioxus Irak4 and Pellementioning

confidence: 57%

“…However, there are few studies on IRAKs from lower vertebrates and invertebrates. But it appears that the composition and function of the IRAK family could be quite different from mammals (25)(26)(27)(28)(29)(30)(31)(32). For example, in lower vertebrates, birds lack IRAK1 and IRAK3, reptiles and amphibians lack IRAK3, and bony fishes lack IRAK2 (33).…”

mentioning

confidence: 99%

Functional Variation of IL-1R–Associated Kinases in the Conserved MyD88–TRAF6 Pathway during Evolution

Yan

Chen

Huang

et al. 2020

The Journal of Immunology

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IL-1R-associated kinases (IRAK) are important regulators in the TLR/IL-1R pathways, but their function appears inconsistent between Drosophila, bony fishes, and vertebrates. This causes a difficulty to understand the IRAK functions. As a step to reveal the evolution of IRAKs, in this study, we performed comparative and functional analysis of IRAKs by exploiting the amphioxus, a pivotal taxon connecting invertebrates and vertebrates. Sequence and phylogenetic analysis indicated three major IRAK lineages: IRAK1/2/3 is a vertebrate-specific lineage, IRAK4 is an ancient lineage conserved between invertebrate and vertebrates, and Pelle is another ancient lineage that is preserved in protostomes and invertebrate deuterostomes but lost in vertebrate deuterostomes. Pelle is closer neither to IRAK4 nor to IRAK1/2/3, hence suggesting no clear functional analogs to IRAK1/2/3 in nonvertebrates. Functional analysis showed that both amphioxus IRAK4 and Pelle could suppress NF-kB activation induced by MyD88 and TRAF6, which are unlike mammalian and Drosophila IRAKs, but, surprisingly, similar to bony fish IRAK4. Also unlike Drosophila IRAKs, no interaction was detected between amphioxus IRAK4 and Pelle, although both of them were shown capable of binding MyD88. These findings, together with previous reports, show that unlike other signal transducers in the TLR/IL-1R pathways, such as MyD88 and TRAF6, the functions of IRAKs are highly variable during evolution and very specialized in different major animal taxa. Indeed, we suggest that the functional variability of IRAKs might confer plasticity to the signal transduction of the TLR/IL-1R pathways, which in return helps the species to evolve against the pathogens.

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Identification and characterization of IL-1 receptor-associated kinase-4 (IRAK-4) in half-smooth tongue sole Cynoglossus semilaevis

Cited by 22 publications

References 31 publications

Aeromonas salmonicidaInfection Only Moderately Regulates Expression of Factors Contributing to Toll-Like Receptor Signaling but Massively Activates the Cellular and Humoral Branches of Innate Immunity in Rainbow Trout (Oncorhynchus mykiss)

Aeromonas salmonicidaInfection Only Moderately Regulates Expression of Factors Contributing to Toll-Like Receptor Signaling but Massively Activates the Cellular and Humoral Branches of Innate Immunity in Rainbow Trout (Oncorhynchus mykiss)

Insights into molecular profiles and genomic evolution of an IRAK4 homolog from rock bream (Oplegnathus fasciatus): Immunogen- and pathogen-induced transcriptional expression

Functional Variation of IL-1R–Associated Kinases in the Conserved MyD88–TRAF6 Pathway during Evolution

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