Molecular cloning and expression analysis of a CXC chemokine gene from large yellow croaker Pseudosciaena crocea

Wan, Xiang; Chen, Xinhua

doi:10.1016/j.vetimm.2008.09.009

Cited by 30 publications

(12 citation statements)

References 27 publications

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“…From the present study, the high-level elevation of CXCR4expression occurred in spleen, kidney and gills, which corresponded well to the major roles of these three tissues played in fish immune system [12, 28, 29]. It might suggest that the CXCR4 was relative to the immune system and it had a high constitutive expression in head kidney, kidney and spleen.…”

Section: Discussionsupporting

confidence: 81%

“…Therefore, the data for turbot CXCR4 obtained here matched to those of the expression of SDF-1. In the large yellow croaker, the expression of SDF-1 was induced initially and then decreased in kidney and spleen postinduction by bacterial vaccine [12]. This expression pattern was mostly matched to the expression of turbot CXCR4 in head kidney and spleen, in which the expression levels were suppressed at the very beginning prior to increasing.…”

Section: Discussionmentioning

confidence: 99%

“…Based on these findings, CXCR4 could serve as a novel biomarker for cancer metastasis and even the inflammatory reaction [11]. CXCR4/SDF-1 interaction is necessary in the immune response [3, 12], and CXCR4 influences the immune system under physiologic and pathologic conditions through negative regulation of MHC class II expression [13]. …”

Section: Introductionmentioning

confidence: 99%

“…Some studies focused on the regulation of chemokines in response to bacterial infection and vaccination, since SDF-1 is thought to play an important role in the first line of defense against pathogens in fish [12, 17]. However, few studies have been performed in fish concerning the expression of its receptor.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus

Jia

Zhang

2009

BioMed Research International

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Chemokine receptor 4 (CXCR4) belongs to the large superfamily of G protein-coupled receptors. The EST sequence of CXCR4 from turbot (Scophthalmus maximus L.) was obtained from a subtractive cDNA library. In the present study, the full-length cDNA sequence of turbot CXCR4 was obtained, and sequence analysis indicated that its primary structure was highly similar to CXCR4 from other vertebrates. Quantitative real-time PCR demonstrated that the highest expression level of turbot CXCR4 was in the spleen following injection with physiological saline (PS). After turbot were challenged with Vibrio harveyi, the lowest expression level of CXCR4 was detected at 8 hours in the spleen and 12 hours in the head kidney, and then increased gradually to 36 hours. These findings suggested that CXCR4 may play a significant role in the immune response of turbot.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus

Jia

Zhang

2009

BioMed Research International

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“…Large yellow croaker, Pseudosciaena crocea , belongs to the family Sciaenidae and represents the largest yield for a single species in Chinese marine net‐cage farming (Wan and Chen ). Because of a close taxonomic relationship, Wang et al () attempted hybridizing P. crocea and M. miiuy , but failed.…”

mentioning

confidence: 99%

Development of Microsatellite Markers for Miiuy Croaker, Miichthys miiuy (Sciaenidae), and Their Application in Verifying Gynogenesis of Large Yellow Croaker, Pseudosciaena crocea, Induced with M. miiuy Sperm

Miao

Chen

et al. 2015

J World Aquaculture Soc

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A total of 30 microsatellite markers were isolated from miiuy croaker, Miichthys miiuy, and characterized. Of these, 24 were polymorphic with an average of 4.33 alleles per locus (range: 2-8). The observed (H o ) and expected (H e ) heterozygosities ranged from 0.0667 to 1.0000 (mean: 0.6361) and 0.3828 to 0.8139 (mean: 0.6443), respectively. The mean polymorphism information content (PIC) value was 0.576, ranging from 0.348 to 0.823. Eight loci deviated significantly from the Hardy-Weinberg equilibrium (HWE). Twelve of the 30 primer pairs amplified DNA sequences of large yellow croaker, Pseudosciaena crocea, and 10 were polymorphic. Except for MM007, sequence alignments of microsatellite flanking regions of the other 11 loci were highly similar between the two species. The gynogenetic P. crocea induced by the sperm of M. miiuy were verified using four cross-amplified primer pairs. No specific paternal bands were found in the offspring, indicating exclusive maternal inheritance.

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Advances in chemokines of teleost fish species

Liu

2024

Aquaculture and Fisheries

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Molecular cloning and expression analysis of a CXC chemokine gene from large yellow croaker Pseudosciaena crocea

Cited by 30 publications

References 27 publications

Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus

Molecular Cloning, Characterization, and Expression Analysis of the CXCR4 Gene from Turbot: Scophthalmus maximus

Development of Microsatellite Markers for Miiuy Croaker, Miichthys miiuy (Sciaenidae), and Their Application in Verifying Gynogenesis of Large Yellow Croaker, Pseudosciaena crocea, Induced with M. miiuy Sperm

Advances in chemokines of teleost fish species

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