Molecular cloning and expression analysis of EIN2, EIN3/EIL, and EBF genes during papaya fruit development and ripening

Zou, Yuan; Ding, Xuezhi; Ye, Lanlan; Zhu, Xiaoyang; Zhang, Lin; Rao, Shen; Chen, Weixin; Li, Xueping

doi:10.1080/01140671.2020.1838571

Cited by 4 publications

(1 citation statement)

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“…The ethylene response is undertaken through a largely linear signaling pathway, in which ethylene is perceived from the membrane‐localized receptors to the essential TFs in the nucleus. As a set of important TFs, EIN3/EIL regulate most ethylene responses in plants, such as leaf senescence in Arabidopsis (Qiu et al, 2015) and ripening and senescence in pear (Shi et al, 2019), peach (Zhang et al, 2019), papaya (Zou et al, 2021), and litchi fruits (Ma et al, 2020). In this context, we found that 13 MaEILs exhibited differential expression profiles during banana fruits under natural, ethylene‐induced and 1‐MCP‐delayed ripening, with MaEIL9 displaying higher expression levels in the ripening (Figures 2A, S1).…”

Section: Discussionmentioning

confidence: 99%

Methionine oxidation and reduction of the ethylene signaling component MaEIL9 are involved in banana fruit ripening

Zhu

Chen

et al. 2022

JIPB

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The ethylene insensitive 3/ethylene insensitive 3‐like (EIN3/EIL) plays an indispensable role in fruit ripening. However, the regulatory mechanism that links post‐translational modification of EIN3/EIL to fruit ripening is largely unknown. Here, we studied the expression of 13 MaEIL genes during banana fruit ripening, among which MaEIL9 displayed higher enhancement particularly in the ripening stage. Consistent with its transcript pattern, abundance of MaEIL9 protein gradually increased during the ripening process, with maximal enhancement in the ripening. DNA affinity purification (DAP)‐seq analysis revealed that MaEIL9 directly targets a subset of genes related to fruit ripening, such as the starch hydrolytic genes MaAMY3D and MaBAM1. Stably overexpressing MaEIL9 in tomato fruit hastened fruit ripening, whereas transiently silencing this gene in banana fruit retarded the ripening process, supporting a positive role of MaEIL9 in fruit ripening. Moreover, oxidation of methionines (Met‐129, Met‐130, and Met‐282) in MaEIL9 resulted in the loss of its DNA‐binding capacity and transcriptional activation activity. Importantly, we identified MaEIL9 as a potential substrate protein of methionine sulfoxide reductase A MaMsrA4, and oxidation of Met‐129, Met‐130, and Met‐282 in MaEIL9 could be restored by MaMsrA4. Collectively, our findings reveal a novel regulatory network controlling banana fruit ripening, which involves MaMsrA4‐mediated redox regulation of the ethylene signaling component MaEIL9.

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