Molecular cloning and expression analysis of a cDNA encoding a glutamate transporter in the honeybee brain

Kucharski, Robert; Ball, Eldon E.; Hayward, David C.; Maleszka, Ryszard

doi:10.1016/s0378-1119(99)00503-x

Cited by 55 publications

(30 citation statements)

References 25 publications

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“…For example, genes encoding a putative inositol 1,4,5-triphosphate receptor (IP 3 R) homologue (Kamikouchi et al, 1998), a Ca 2ϩ /calmodulindependent protein kinase II (CaMKII; Kamikouchi et al, 2000), and the putative transcription factor mushroom body large-type Kenyon cell-specific protein-1 (Mblk-1; Takeuchi et al, 2001) have all been reported to be expressed preferentially within NCCs, whereas the putative glutamate transporter Am-EAAT is most prominently expressed in ICCs of the MBs (Kucharski et al, 2000). Although division of Kenyon cells into OCCs, ICCs, and NCCs has proved useful for describing the expression patterns of Amdop1 and Amdop2, these three major cell groupings do not represent fully the heterogeneity that exists among MB intrinsic neurons.…”

Section: Implications Of This Work For Future Studiesmentioning

confidence: 99%

Developmental changes in expression patterns of two dopamine receptor genes in mushroom bodies of the honeybee, Apis mellifera

Kurshan

Hamilton

Mustard

et al. 2003

J of Comparative Neurology

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The expression patterns of two dopamine receptor genes, Amdop1 and Amdop2, in the developing mushroom bodies of the honeybee brain were determined by using in situ hybridisation. Both genes were expressed throughout pupal development, but their patterns of expression in the three major divisions of mushroom body intrinsic neurons (outer compact cells, noncompact cells, and inner compact cells) were quite distinct. Amdop1 expression could be detected in all three mushroom body cell groups throughout development. Staining for Amdop1 mRNA was particularly intense in newly born Kenyon cells, suggesting that levels of Amdop1 expression are higher in newborn cells than in more mature mushroom body neurons. This was not the case for Amdop2. Amdop2 expression in the mushroom bodies was restricted to inner and outer compact cells during most of pupal development, appearing in noncompact cells only late in metamorphosis or at adult eclosion. In contrast to the case with Amdop1, staining for Amdop2 mRNA was observed in glial cells. Expression of Amdop2 in glial cells was detected only at early stages of glial cell development, when the cells are reported to be actively dividing. This study not only implicates dopamine in the development of honeybee mushroom bodies but also suggests different roles for the two dopamine receptors investigated.

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Section: Implications Of This Work For Future Studiesmentioning

confidence: 99%

Developmental changes in expression patterns of two dopamine receptor genes in mushroom bodies of the honeybee, Apis mellifera

Kurshan

Hamilton

Mustard

et al. 2003

J of Comparative Neurology

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“…Glutamate-like immunoreactivity (Bicker et al, 1988;Schür-mann et al, 2000;Sinakevitch et al, 2001) and glutamateinduced ion currents (Cayre et al, 1999) were described in insect CNS neurons. Moreover, molecular studies identified the expression of glutamate transporters and glutamate receptors homologous to mammalian ones (Ultsch et al, 1993;Parmentier et al, 1996;Seal et al, 1998;Kucharski et al, 2000;Volkner et al, 2000;Funada et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Focal and Temporal Release of Glutamate in the Mushroom Bodies Improves Olfactory Memory inApis mellifera

Locatelli

Bundrock²,

Müller³

2005

J. Neurosci.

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In contrast to vertebrates, the role of the neurotransmitter glutamate in learning and memory in insects has hardly been investigated. The reason is that a pharmacological characterization of insect glutamate receptors is still missing; furthermore, it is difficult to locally restrict pharmacological interventions. In this study, we overcome these problems by using locally and temporally defined photo-uncaging of glutamate to study its role in olfactory learning and memory formation in the honeybee, Apis mellifera. Uncaging glutamate in the mushroom bodies immediately after a weak training protocol induced a higher memory rate 2 d after training, mimicking the effect of a strong training protocol. Glutamate release before training does not facilitate memory formation, suggesting that glutamate mediates processes triggered by training and required for memory formation. Uncaging glutamate in the antennal lobes shows no effect on memory formation. These results provide the first direct evidence for a temporally and locally restricted function of glutamate in memory formation in honeybees and insects.

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“…In particular, insect EAAT homologues have been cloned by independent groups from the brain and embryo of Drosophila melanogaster (dEAAT1 and dEAAT2) as well as the brains of caterpillar Trichoplusia ni (TrnEAAT), cockroach Diploptera punctata (DipEAAT) and honeybee Apis mellifera (AmEAAT) (Besson et al, 1999;Donly et al, 1997Donly et al, , 2000Kawano et al, 1999;Kucharski et al, 2000;. With the exception of AmEAAT, all insect homologues cloned thus far have been heterologously expressed and functionally characterized as Na + -dependent high-affinity glutamate and aspartate transporters with affinity in the micromolar range (Besson et al, 1999(Besson et al, , 2000Donly et al, 1997Donly et al, , 2000Kawano et al, 1999;Kucharski et al, 2000;. Electrophysiological studies on dEAAT1 have illustrated a substrate-elicited anion conductance that is stoichiometrically uncoupled from substrate translocation, much like the mammalian EAATs (Fairman et al, 1995;Wadiche et al, 1995b).…”

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Functional characterization of a glutamate/aspartate transporter from the mosquitoAedes aegypti

Umesh

Cohen

Ross

et al. 2003

Journal of Experimental Biology

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SUMMARYGlutamate elicits a variety of effects in insects, including inhibitory and excitatory signals at both neuromuscular junctions and brain. Insect glutamatergic neurotransmission has been studied in great depth especially from the standpoint of the receptor-mediated effects, but the molecular mechanisms involved in the termination of the numerous glutamatergic signals have only recently begun to receive attention. In vertebrates, glutamatergic signals are terminated by Na+/K+-dependent high-affinity excitatory amino acid transporters (EAAT), which have been cloned and characterized extensively. Cloning and characterization of a few insect homologues have followed, but functional information for these homologues is still limited. Here we report a study conducted on a cloned mosquito EAAT homologue isolated from the vector of the dengue virus, Aedes aegypti. The deduced amino acid sequence of the protein, AeaEAAT,exhibits 40–50% identity with mammalian EAATs, and 45–50% identity to other insect EAATs characterized thus far. It transports l-glutamate as well as l- and d-aspartate with high affinity in the micromolar range, and demonstrates a substrate-elicited anion conductance when heterologously expressed in Xenopus laevis oocytes, as found with mammalian homologues. Analysis of the spatial distribution of the protein demonstrates high expression levels in the adult thorax, which is mostly observed in the thoracic ganglia. Together, the work presented here provides a thorough examination of the role played by glutamate transport in Ae. aegypti.

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Molecular cloning and expression analysis of a cDNA encoding a glutamate transporter in the honeybee brain

Cited by 55 publications

References 25 publications

Developmental changes in expression patterns of two dopamine receptor genes in mushroom bodies of the honeybee, Apis mellifera

Developmental changes in expression patterns of two dopamine receptor genes in mushroom bodies of the honeybee, Apis mellifera

Focal and Temporal Release of Glutamate in the Mushroom Bodies Improves Olfactory Memory inApis mellifera

Functional characterization of a glutamate/aspartate transporter from the mosquitoAedes aegypti

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