Molecular cloning and circadian expression profile of insect neuropeptide PDF in black blowfly, Phormia regina

Matsushima, Ayami; Yokotani, Satoru; Lui, Xiaohui; Sumida, Kazunori; Honda, Takeshi; Sato, Seiji; Kaneki, Atsushi; Takeda, Yukimasa; Chuman, Yoshiro; Ozaki, Mamiko; Asai, Daisuke; Nose, Takeru; Onoue, Hitoshi; Ito, Yushi; Tominaga, Yoshiya; Shimohigashi, Yasuyuki; Shimohigashi, Miki

doi:10.1007/s10989-004-2396-5

Cited by 3 publications

(4 citation statements)

References 23 publications

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“…Since I and L are similar in physico-chemical properties and size, we predict that a substitution of position 4 of Lem-PDF with either I or L will only slightly change the peptide's bioactivity. Molecular cloning based on sequence homology of the mRNA for β-PDH and pigment-dispersing factor has recently led to the identification of pigment-dispersing factor in several insect species ( Chuman et al, 2002 ; Sato et al, 2002 ; Matsushima et al, 2004a , b ), and could also be used to clarify the full sequence of Lem-PDF. On the other hand, the occurrence of the pigment-dispersing factor peptides predicted from cDNA cloning still has to be verified biochemically in those insects where only the genes were cloned.…”

Section: Discussionmentioning

confidence: 99%

“…Insect pigment-dispersing factors are neuropeptides with a highly conserved 18 amino acid sequence, and are homologues to the pigment-dispersing hormones (PDH) of crustaceans ( Rao and Riehm, 1988 ). To date, pigment-dispersing factors have been identified in several dipteran insect species as well as in Orthopteromorpha and Hemiptera ( Rao et al, 1987 ; Rao and Riehm, 1988 ; Mohrherr et al, 1991 , 1994 ; Nässel et al, 1993 ; Park and Hall, 1998 ; Sato et al, 2002 ; Chuman et al, 2002 ; Singaravel et al, 2003 ; Matsushima et al, 2004a , b ).…”

Section: Introductionmentioning

confidence: 99%

“…Pigment-dispersing factor immunostaining in the terminals of the sLN v s in the dorsal protocerebrum but not within the somata exhibits a daily and circadian cycle with a peak during early morning, which apparently corresponds to the daily locomotor activity of D. melanogaster ( Park et al, 2000 ). Since the amount of pdf mRNA does not exhibit daily cycles ( Park et al, 2000 ; Matsushima et al, 2004b ), it is likely that the release of pigment-dispersing factor from the dorsal terminals of the s-LN v s is regulated in a circadian fashion.…”

Section: Introductionmentioning

confidence: 99%

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Chronobiological analysis and mass spectrometric characterization of pigment-dispersing factor in the cockroach Leucophaea maderae

Hamasaka

Mohrherr

Predel

et al. 2005

Journal of Insect Science

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Pigment-dispersing factor (PDF) is a neuropeptide that plays a prominent role in the circadian clock of several insects. The cockroach Leucophaea maderae was the first animal where the site of a biological clock could be located, and still is a focal point of circadian research. Although detailed studies on the action of pigment-dispersing factor and the distribution of PDF-like immunoreactivity in the L. maderae brain exist, a native pigment-dispersing factor of this species has not been characterized so far. The authentic Lem-PDF was isolated from L. maderae by a combination of high performance liquid chromatography, crab pigment-dispersion bioassay and an immunosorbent assay. Mass spectrometric characterization and the conserved sequence of pigment-dispersing factor in orthopteromorphan insects suggest that Lem-PDF has the sequence NSEXINSLLGLPKVLNDAa (where X= I or L). Lem-PDF is thus identical to either Periplaneta americana PDF or Acheta domesticus PDF. Detailed analysis of PDF-like immunofluorescence in different regions of the brain suggests that there are no drastic daily changes in the amount of pigment-dispersing factor as occur in Drosophila melanogaster, which might be explained by a lack of circadian pigment-dispersing factor release and production, or by phase differences between the pigment-dispersing factor neurons.AbbreviationsESI-Q-TOF MS electrospray ionization-quadrupole-time of flight mass spectrometryMALDI-TOF MS matrix-assisted laser desorption ionization-time of flight mass spectrometryPDF pigment-dispersing factor (Drm-PDF Drosophila melanogaster-PDF; Lem-PDF, Leucophaea maderae-PDF; Pea-PDF, Periplaneta americana-PDF)PDH pigment-dispersing hormones-LNvs and lLNvs small and large ventral lateral neuronsZT Zeitgeber time

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chronobiological analysis and mass spectrometric characterization of pigment-dispersing factor in the cockroach Leucophaea maderae

Hamasaka

Mohrherr

Predel

et al. 2005

Journal of Insect Science

View full text Add to dashboard Cite

show abstract

“…Pigment-dispersing factor immunostaining in the terminals of the sLN v s in the dorsal protocerebrum but not within the somata exhibits a daily and circadian cycle with a peak during early morning, which apparently corresponds to the daily locomotor activity of D. melanogaster (Park et al, 2000). Since the amount of pdf mRNA does not exhibit daily cycles Matsushima et al, 2004b), it is likely that the release of pigment-dispersing factor from the dorsal terminals of the s-LN v s is regulated in a circadian fashion.…”

Section: Insectmentioning

confidence: 97%

Chronobiological analysis and mass spectrometric characterization of pigment-dispersing factor in the cockroach Leucophaea maderae

2005

Journal of Insect Science

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Structural isoforms of the circadian neuropeptide PDF expressed in the optic lobes of the cricket Gryllus bimaculatus: Immunocytochemical evidence from specific monoclonal antibodies

Honda

Matsushima

Sumida

et al. 2006

J of Comparative Neurology

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Pigment-dispersing factor (PDF) is an 18-mer peptide that acts as a principal neurotransmitter of the insect circadian clock. Our previous study, utilizing anti-Uca beta-PDH polyclonal antibody (pAb) to immunolabel the optic lobe of the cricket Gryllus bimaculatus, suggested the existence of an alternative PDF-like peptide in the outer cells of the first neuropile, or lamina (La), which were much less immunoreactive than the inner cells of the second neuropile, the medulla (Me). To obtain structural information about such a PDF-like peptide, we prepared 10 anti-Gryllus PDF monoclonal (mAb) and pAb antibodies and analyzed their detailed epitope specificities. The PDFMe and PDFLa inner cells and their axonal projections were clearly immunoreactive to all these antibodies, revealing the widespread immunocytochemical organization of the PDF system in the optic lobe, as seen previously with anti-Uca beta-PDH pAb and anti-Gryllus PDF mAb, the epitope structures of which were also clarified in this study. The lamina outer cells, which we found lacked a target pdf mRNA, displayed specific immunoreactivities, indicating that the cells contain a distinct PDF-like peptide possessing both N- and C-terminal structures. These cells were not immunolabeled by some other monoclonal antibodies, however, implying that the PDFLa outer cells have a PDF isoform peptide devoid of Asn at positions 6 and 16. This isoform was also identified in a varicose arborization in the lamina. These results suggest not only the structure of the peptide, but also the possibility of additional functions of this novel PDF isoform.

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Molecular cloning and circadian expression profile of insect neuropeptide PDF in black blowfly, Phormia regina

Cited by 3 publications

References 23 publications

Chronobiological analysis and mass spectrometric characterization of pigment-dispersing factor in the cockroach Leucophaea maderae

Chronobiological analysis and mass spectrometric characterization of pigment-dispersing factor in the cockroach Leucophaea maderae

Chronobiological analysis and mass spectrometric characterization of pigment-dispersing factor in the cockroach Leucophaea maderae

Structural isoforms of the circadian neuropeptide PDF expressed in the optic lobes of the cricket Gryllus bimaculatus: Immunocytochemical evidence from specific monoclonal antibodies

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