Chronobiological analysis and mass spectrometric characterization of pigment-dispersing factor in the cockroach Leucophaea maderae

Hamasaka, Yasutaka; Mohrherr, Carl J.; Predel, Reinhard; Wegener, Christian

doi:10.1093/jis/5.1.43

Cited by 11 publications

(7 citation statements)

References 28 publications

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“…In this study, we could reproduce this pattern using a characterized polyclonal antiserum produced against Drosophila PDF [76], [77], with hitherto undescribed dendritic arborizations that were visualized by pdf -GAL4-directed GFP-expression as described below.…”

Section: Resultsmentioning

confidence: 99%

Neuroarchitecture of Peptidergic Systems in the Larval Ventral Ganglion of Drosophila melanogaster

et al. 2007

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Recent studies on Drosophila melanogaster and other insects have revealed important insights into the functions and evolution of neuropeptide signaling. In contrast, in- and output connections of insect peptidergic circuits are largely unexplored. Existing morphological descriptions typically do not determine the exact spatial location of peptidergic axonal pathways and arborizations within the neuropil, and do not identify peptidergic in- and output compartments. Such information is however fundamental to screen for possible peptidergic network connections, a prerequisite to understand how the CNS controls the activity of peptidergic neurons at the synaptic level. We provide a precise 3D morphological description of peptidergic neurons in the thoracic and abdominal neuromeres of the Drosophila larva based on fasciclin-2 (Fas2) immunopositive tracts as landmarks. Comparing the Fas2 “coordinates” of projections of sensory or other neurons with those of peptidergic neurons, it is possible to identify candidate in- and output connections of specific peptidergic systems. These connections can subsequently be more rigorously tested. By immunolabeling and GAL4-directed expression of marker proteins, we analyzed the projections and compartmentalization of neurons expressing 12 different peptide genes, encoding approximately 75% of the neuropeptides chemically identified within the Drosophila CNS. Results are assembled into standardized plates which provide a guide to identify candidate afferent or target neurons with overlapping projections. In general, we found that putative dendritic compartments of peptidergic neurons are concentrated around the median Fas2 tracts and the terminal plexus. Putative peptide release sites in the ventral nerve cord were also more laterally situated. Our results suggest that i) peptidergic neurons in the Drosophila ventral nerve cord have separated in- and output compartments in specific areas, and ii) volume transmission is a prevailing way of peptidergic communication within the CNS. The data can further be useful to identify colocalized transmitters and receptors, and develop peptidergic neurons as new landmarks.

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Section: Resultsmentioning

confidence: 99%

Neuroarchitecture of Peptidergic Systems in the Larval Ventral Ganglion of Drosophila melanogaster

et al. 2007

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“…PDF is an 18 amino acid neuropeptide that is essential for maintained locomotor activity rhythms in Drosophila (Renn et al, 1999; Helfrich-Förster, 2005). PDF homologues (which usually differ only by 2 amino acid substitutions) have been identified in diverse insect species (reviewed by Hamasaka et al, 2005). Both examined species of moths possess 4 small but clearly stained cells in the Pfv region of the OL (Fig.…”

Section: Discussionmentioning

confidence: 99%

Is the Sex Communication of Two Pyralid Moths, Plodia interpunctella and Ephestia kuehniella, under Circadian Clock Regulation?

et al. 2012

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Females of the Indian meal moth, Plodia interpunctella, and females of the Mediterranean flour month, Ephestia kuehniella (both Lepidoptera: Pyralidae), exhibit daily rhythms in calling behavior. The peak in P. interpunctella calling occurs at dusk, whereas E. kuehniella calls preferentially at dawn. This behavior turned arrhythmic in P. interpunctella females in constant darkness (DD) and remained arrhythmic in constant light (LL), whereas E. kuehniella females showed a persistent rhythm in DD and suppression of the behavior in LL, indicating regulation by a circadian clock mechanism. The rhythm of male locomotor activity corresponded well with the sexual activity of females, reaching the peak at dusk in P. interpunctella and at dawn in E. kuehniella. An immunohistochemical study of the pheromone biosynthesis activating neuropeptide, corazonin, and pigment dispersing factor revealed distinct sets of neurons in the brain-subesophageal complex and in the neurohemal organs of the 2 species.

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“…How likely is coexpression of three or more neuropeptides in single neurons? The peptide PDF has been characterized in the cockroach L. maderae (Hamasaka et al 2005 ), and homologous PDF-ir neurons and respective conserved PDF-peptides have been described in other species (Dircksen et al 1987 ; Park and Hall 1998 ; Chuman et al 2002 ; Matsushima et al 2004 ; Honda et al 2006 ). Therefore, the anti- Drosophila -PDF monoclonal antibody employed in this study most probably recognizes only the PDF peptide in PDFMe neurons, especially as it shows the same labeling pattern as the well-characterized anti- Uca -PDH polyclonal antibody from Dircksen et al ( 1987 ).…”

Section: Discussionmentioning

confidence: 99%

“…This antiserum has been widely used to detect PDF-expressing neurons in many insect species and has recently been systematically characterized by Honda et al ( 2006 ). Furthermore, a PDF peptide was previously isolated in L. maderae by Hamasaka et al ( 2005 ). The anti-β-PDH antiserum was used at a working dilution of 1:15,000.…”

Section: Methodsmentioning

confidence: 99%

Circadian pacemaker coupling by multi-peptidergic neurons in the cockroach Leucophaea maderae

2011

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Lesion and transplantation studies in the cockroach, Leucophaea maderae, have located its bilaterally symmetric circadian pacemakers necessary for driving circadian locomotor activity rhythms to the accessory medulla of the optic lobes. The accessory medulla comprises a network of peptidergic neurons, including pigment-dispersing factor (PDF)-expressing presumptive circadian pacemaker cells. At least three of the PDF-expressing neurons directly connect the two accessory medullae, apparently as a circadian coupling pathway. Here, the PDF-expressing circadian coupling pathways were examined for peptide colocalization by tracer experiments and double-label immunohistochemistry with antisera against PDF, FMRFamide, and Asn13-orcokinin. A fourth group of contralaterally projecting medulla neurons was identified, additional to the three known groups. Group one of the contralaterally projecting medulla neurons contained up to four PDF-expressing cells. Of these, three medium-sized PDF-immunoreactive neurons coexpressed FMRFamide and Asn13-orcokinin immunoreactivity. However, the contralaterally projecting largest PDF neuron showed no further peptide colocalization, as was also the case for the other large PDF-expressing medulla cells, allowing the easy identification of this cell group. Although two-thirds of all PDF-expressing medulla neurons coexpressed FMRFamide and orcokinin immunoreactivity in their somata, colocalization of PDF and FMRFamide immunoreactivity was observed in only a few termination sites. Colocalization of PDF and orcokinin immunoreactivity was never observed in any of the terminals or optic commissures. We suggest that circadian pacemaker cells employ axonal peptide sorting to phase-control physiological processes at specific times of the day.

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Chronobiological analysis and mass spectrometric characterization of pigment-dispersing factor in the cockroach Leucophaea maderae

Cited by 11 publications

References 28 publications

Neuroarchitecture of Peptidergic Systems in the Larval Ventral Ganglion of Drosophila melanogaster

Neuroarchitecture of Peptidergic Systems in the Larval Ventral Ganglion of Drosophila melanogaster

Is the Sex Communication of Two Pyralid Moths, Plodia interpunctella and Ephestia kuehniella, under Circadian Clock Regulation?

Circadian pacemaker coupling by multi-peptidergic neurons in the cockroach Leucophaea maderae

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