Molecular cloning and characterization of GhERF105, a gene contributing to the regulation of gland formation in upland cotton (Gossypium hirsutum L.)

Wu, Chaofeng; Cheng, Hongyan; Li, Shuyan; Zuo, Dongyun; Lin, Zhongxu; Zhang, Youping; Lv, Limin; Wang, Qiaolian; Song, Guoli

doi:10.1186/s12870-021-02846-5

Cited by 12 publications

(12 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cotton ( Gossypium spp.) is one of the most important crops in the world due to its economic value in textile fiber, feed, foodstuff, oil, and biofuel products ( Wu et al, 2021 ). Cotton plantation in Xinjiang is one of the most important cotton production regions worldwide and plays an important role in the economy.…”

Section: Introductionmentioning

confidence: 99%

Pseudomonas spp. Enriched in Endophytic Community of Healthy Cotton Plants Inhibit Cotton Verticillium Wilt

et al. 2022

View full text Add to dashboard Cite

The plant microbiome plays a fundamental role in plant growth and health. However, detailed information regarding the plant endophytic microbiome during the infection period of a pathogen is largely unknown. Here, we investigated the microbial community of healthy and diseased cotton plants and the root exudate profiles of susceptible and resistant cultivars utilizing high-throughput sequencing and metabolomics. The results showed that the pathogen infection reduced bacterial diversity and significantly affected the bacterial community composition. The microbiome assembly is shaped predominantly by cultivars. The endophytic microbiome of the infected plants showed greater complexity than the healthy plants in network analysis. The results displayed that a total of 76 compounds were significantly different in the two groups, with 18 compounds showing a higher relative abundance in the resistant cultivars and 58 compounds in the susceptible cultivars. Pathway enrichment analysis showed that pathways related to plant hormone signal transduction, biosynthesis of various secondary metabolites, and biosynthesis and metabolism of amino acids were prominently altered. We also demonstrate that plants inoculated with Pseudomonas sp. strains showed increased resistance to the cotton Verticillium wilt compared with the control plants in pot experiments. Overall, it showed that the pathogen infection affected the community composition, and healthy plants displayed an enriched beneficial microbiome to combat the plant disease. These findings significantly advance our understanding of the endophytic microbiome assembly under the pathogen infection and develop microbiome-based solutions for sustainable crop production systems.

show abstract

Section: Introductionmentioning

confidence: 99%

Pseudomonas spp. Enriched in Endophytic Community of Healthy Cotton Plants Inhibit Cotton Verticillium Wilt

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The thermocycle procedure was as follows: 95°C for 3 minutes, followed by 40 cycles of 95°C for 5 seconds and 60°C for 15 seconds. All the data were normalized to those of actin ( Wu et al., 2021 ), which served as an internal reference gene, and the relative expression of all the evaluated GhNPF genes was calculated using the 2 -ΔΔCt method ( Willems et al., 2008 ). After normalization of the data from three independent experiments, all the data were expressed as the mean ± standard error.…”

Section: Methodsmentioning

confidence: 99%

Genome-wide investigation and expression profiles of the NPF gene family provide insight into the abiotic stress resistance of Gossypium hirsutum

et al. 2023

View full text Add to dashboard Cite

Membrane transporters encoded by NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER (NPF) genes, which play crucial roles in plant growth, development and resistance to various stresses, are involved in the transport of nitrate (NO3-) and peptides. In several plant species, NPF genes are involved in the resistance to abiotic stresses; however, whether the whole NPF gene family in cotton contributes to this resistance has not been systematically investigated. Here, 201 genes encoding NPF proteins with a peptide transporter (PTR) domain were confirmed in three different Gossypium species, namely, Gossypium hirsutum, Gossypium arboreum and Gossypium raimondii. The NPF proteins in these three Gossypium species and Arabidopsis thaliana were classified into three different subfamilies via phylogenetic analysis. Among the genes that encode these proteins, most GhNPF genes in the same subfamily contained similar gene structures and conserved domains. Predictions of the promoters of these genes revealed that the cis-acting elements included phytohormone- and light-responsive elements, indicating that some of these genes might be expressed in response to abiotic stress. Furthermore, 52 common potential candidate genes in 98 GhNPFs were predicted to exhibit specific spatiotemporal expression patterns in different tissues based on two RNA sequencing (RNA-seq) datasets. Finally, the gene expression profiles of abiotic stress indicated that 31 GhNPF genes were upregulated in at least one treatment period. Under abiotic stress for 12 and 24 h, the expression of GhNPF8 was upregulated upon cold treatment but downregulated with heat treatment, salt treatment and drought treatment. Furthermore, the expression of genes GhNPF8, GhNPF54 and GhNPF43 peaked at 6 h after heat and salt treatment. These results indicated that these genes exhibit underlying characteristics related to responses to abiotic stress. The verification of NPFs and analysis of their expression profiles in different tissues and in response to different abiotic stresses of cotton provide a basis for further studying the relationship between abiotic stress resistance and nitrogen (N) transport in cotton, as well as identifying candidate genes to facilitate their functional identification.

show abstract

“…The full-length cDNA of GhERF105 and other genes was amplified from the leaves of CCRI12 and cloned into the relative vector (such as pBI121, pGBKT7 et al) as described previously ( Wu et al 2021). The PCR products were electrophoresed on a 1% agarose gel, then were purified following the instructions in the QIAquick PCR Purification Kit (250) (Qiagen, Düsseldorf, Germany) and eluted in a final sample volume of 35 µL of Qiagen EB buffer.…”

Section: Molecular Cloning Of the Full-length Cdnamentioning

confidence: 99%

Molecular cloning and characterization of an AP2/ERF protein gene in cotton (Gossypium hirsutumL.)

2023

Preprint

Self Cite

View full text Add to dashboard Cite

Upland cotton (Gossypium hirsutum L.) is one of the most economically important crops worldwide due to the significant source of natural fiber, feed, oil and biofuel products. Cottonseed can also serve as an excellent source of edible protein and oil. However,the presence of gossypol in pigment gland has limited it utilization. In the past few decades, some progress has been made in the understanding molecular mechanism of the formation of the pigment gland. However, little is known about the specific mechanism of pigment gland formation in cotton. In this study, the cDNA sequence of a ethylene transcription factor gene, designated GhERF105a, was cloned from upland cotton CCRI12. Sequence alignment revealed that GhERF105a gene contained a typical AP2/ERF domain of 61 amino acids, and belonged to the ERF subgroup of the ERF supfamily. It was highly expressed in the leaves and stems of glanded plants but had substantially lower expression of the glandless plants. GhERF105a, localized to the nucleus, could bind to GCC-box and DRE. Some development, phytohormone and stress related cis-elements were enriched in the promoters of GhERF105a/d. Split ubiquitin assays in yeast and BiFC experiments showed extensive interactions between GhERF105a and Gh_A07G1044. In addition, GhERF105a was highly similar with GhERF105d in the gene length, molecular weight, protein molecule, gene structure and expression pattern. The overall results suggested that GhERF105a might participate in the pigment gland formation and stree-response processes.

show abstract

Molecular cloning and characterization of GhERF105, a gene contributing to the regulation of gland formation in upland cotton (Gossypium hirsutum L.)

Cited by 12 publications

References 59 publications

Pseudomonas spp. Enriched in Endophytic Community of Healthy Cotton Plants Inhibit Cotton Verticillium Wilt

Pseudomonas spp. Enriched in Endophytic Community of Healthy Cotton Plants Inhibit Cotton Verticillium Wilt

Genome-wide investigation and expression profiles of the NPF gene family provide insight into the abiotic stress resistance of Gossypium hirsutum

Molecular cloning and characterization of an AP2/ERF protein gene in cotton (Gossypium hirsutumL.)

Contact Info

Product

Resources

About