Molecular cloning and characterization of sea bass (Dicentrarchus labrax, L.) Tapasin

Pinto, Rute D.; Silva, Diogo V. da; Pereira, Pedro José Barbosa; Santos, Nuno M.S. dos

doi:10.1016/j.fsi.2011.10.029

Cited by 4 publications

(2 citation statements)

References 61 publications

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“…CRT binds Zn 2+ (Baksh et al 1995) with low affinity , presumably through five highly conserved histidine residues located at its Ndomain (Baksh et al 1995 (Pinto et al 2013). However, only codon 118 and 123 resulted negatively selected in these analyses.…”

Section: Positively Selected Sites Identificationsmentioning

confidence: 97%

“…The overall shape of CRT includes a globular structure (N/C domains) (Kozlov et al 2010;Chouquet et al 2011;Pocanschi et al 2011) and an extended arm-like hairpin structure (P domain) (Ellgaard et al 2001). In comparison to present information about the structure and the functions of CRTs, little is known about its evolution (Pinto et al 2013). Evolution aspects are very important in studying the structure and function of a protein (Gaston & Roger 2013).…”

Section: Introductionmentioning

confidence: 99%

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Calreticulin molecular evolution: a strong purifying and episodic diversifying selection result

Bakiu

2014

Biologia

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Calreticulin (CRT) is a low molecular weight protein present in vertebrates, invertebrates and higher plants.Its multiple functions have been demonstrated. It plays an important role as a chaperone and Ca 2+ buffer inside sarcoplasmic/endoplasmic reticulum (SR/ER), and outside the ER in many physiological/pathological processes. Recently it has been observed that CRT over-expression or its absence is linked to various pathological conditions, such as malignant evolution and progression, and these facts really increased its study interests. Using an evolution approach CRT was further characterized. Several Bayesian phylogenetic analyses were performed using coding and amino acid sequences. CRT molecular evolution was investigated for the presence of negative or/and positive selection using HyPhy package. The results indicated that the purifying selection might have operated over the whole CRT primary structure. Although, an episodic diversifying selection was also found on the analyzed CRT sequences.

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Section: Positively Selected Sites Identificationsmentioning

confidence: 97%