Molecular cloning and characterization of chicken lipopolysaccharide-induced TNF-α factor (LITAF)

Hong, Yeong Ho; Lillehoj, Hyun S.; Lee, Sung Hyen; Park, Dong Woon; Lillehoj, Erik P.

doi:10.1016/j.dci.2005.12.007

Cited by 119 publications

(62 citation statements)

References 33 publications

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“…Expression of LITAF is principally in the spleen of chickens, as well as in intestinal intraepithelial lymphocytes. The LITAF protein is a transcription factor that mediates the expression of members of the tumor necrosis factor ligand superfamily [39]. On d20, expression of LITAF was reduced due to both probiotic administration methods; when coupled with the decrease in IFN-γ levels, it further supports the thought that probiotics may promote an anti-inflammatory environment, suppress Th1 activity, and promote a Th2 mediated response.…”

Section: Edens Et Al Compared the Hatchability Of Broiler Embryos Inmentioning

confidence: 48%

In ovo and Dietary Supplementation of Probiotics Affects Post-Hatch Expression of Immune-Related Genes in Broiler Chicks

Pender¹,

Kim²,

Sumners³

et al. 2017

J immuno Biol

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During the first week post-hatch, the neonatal chick is immunologically vulnerable and subject to infectious threats found in the environment. Probiotics are live, non-pathogenic microorganisms known to have a positive effect on the host by improving the natural balance of gut microbiota and promoting animal health. The objective of this study was to determine the effects of administering probiotics in ovo and in the diet on broiler chick hatchability, and post-hatch immune organ weights and ileal immune-related gene expression. At embryonic day 18, 1584 eggs were injected with nothing (Dry), 1 × 10 6 , or 1 × 10 7 (P1 and P2 respectively) probiotic bacteria. The remaining 393 eggs were left non-injected to serve as a negative control. Immune organ weights and tissue samples were taken on DOH and d4, 6, 8, 14, and 20. No differences were observed for hatchability or relative bursa weights. Only on d6, the P2 birds receiving the probiotic-supplemented diet had larger spleens as a result of a 2-way interaction between in ovo treatment and post-hatch diet. Both in ovo and dietary administration of probiotics were able to modulate the expression of the immune-related genes in the ileum; however, expression patterns differed based on the gene, treatment, and time point evaluated. In conclusion, these results indicate that in ovo supplementation of this commercial probiotic product does not influence hatchability and is capable of differentially modulating expression of certain genes in the ileum. Furthermore, in ovo administration of probiotics has an effect similar to that of dietary supplementation endorsing its usage to potentially promote early colonization of beneficial bacteria to stimulate intestinal and immune system development.

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Section: Edens Et Al Compared the Hatchability Of Broiler Embryos Inmentioning

confidence: 48%

In ovo and Dietary Supplementation of Probiotics Affects Post-Hatch Expression of Immune-Related Genes in Broiler Chicks

Pender¹,

Kim²,

Sumners³

et al. 2017

J immuno Biol

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“…TNF-␣ in mammals is a member of a group of NF-B-activated signaling cytokines that stimulate systemic inflammation. In chickens, the production of a TNF-␣-modulating factor has been shown to increase, along with the production of inflammatory cytokines, after exposure to Eimeria and Salmonella species (6,11,17,29). Given the inflammatory nature of NE disease and the predominance of innate and inflammatory responses observed in the present study, TNF-␣-inducing factor homolog expression may be indicative of the initiation of a TNF-␣-type response in chickens infected with C. perfringens (2,13).…”

Section: Vol 16 2009 Tlr Expression In C Perfringens-challenged Chmentioning

confidence: 75%

Expression Profiles of Genes in Toll-Like Receptor-Mediated Signaling of Broilers Infected with Clostridium perfringens

Lü

Sarson

Gong

et al. 2009

Clin Vaccine Immunol

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Toll-like receptors (TLRs) participate in detecting microbial pattern molecules for activation of the host immune response. We investigated possible roles of TLRs in the chicken response to Clostridium perfringens infection by examining the expression of TLR genes and other genes involved in TLR-mediated signaling within the spleens and ilea of C. perfringens-challenged broilers. Upregulation of a tumor necrosis factor alphainducing factor homolog in challenged chickens compared to naïve chickens was observed, regardless of the incidence of necrotic enteritis. In addition, the members of the TLR2 subfamily were found to be most strongly involved in the host response to C. perfringens challenge, although the expression of TLR4 and TLR7 was also upregulated in spleen tissues. While the combination of TLR1.2, TLR2.1, and TLR15 appeared to play a major role in the splenic response, the expression of TLR2.2 and TLR1.1 was positively correlated to the expression of adaptor molecules MyD88, TRAF6, TRIF, and receptor interacting protein 1 in the ileal tissues, demonstrating a dynamic spatial and temporal innate host response to C. perfringens.

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“…The proinflammatory cytokines IL-6, TNF-α, and iNOS, which are widely expressed in lymphoid (thymus) and nonlymphoid (liver) tissues, are the most powerful cognitive pathways in response to inflammatory process [17]. Supplementation of rats exposed to cyclic heat stress with vitamin C simultaneously decreased the mRna levels of HSP70 and inflammatory cytokines (IL-6 and TNF-α) only in the liver compared with heat-stressed rats.…”

Section: Discussionmentioning

confidence: 99%

Effects of Cyclic Heat Stress or Vitamin C Supplementation during Cyclic Heat Stress on HSP70, Inflammatory Cytokines, and the Antioxidant Defense System in Sprague Dawley Rats

et al. 2012

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A total of 21 male SD rats were divided into three groups to investigate the effects of consecutive cyclic heat stress or vitamin C under heat stress on heat shock protein (HSP) 70, inflammatory cytokines, and antioxidant systems. The heat stress (HS) and vitamin C supplementation during heat stress (HS+VC) groups were exposed to cyclic heat stress (23 to 38 to 23°C) for 2 h on each of seven consecutive days. The HS+VC group had free access to water containing 0.5% vitamin C throughout the experiment. Hepatic HSP70 mRNA in the HS group was significantly (P<0.05) higher than that in the control (CON) or HS+VC group. The mRNA levels of tumor necrosis factor (TNF)-α and inducible nitric oxide synthase (iNOS) in the HS group were greater (P<0.05) than those in the CON group. The HS+VC group showed significantly (P<0.05) lower mRNA levels of hepatic interleukin-6 and TNF-α than the HS group. However, thymic HSP70 and inflammatory cytokines were unaffected by treatments. In the hepatic antioxidant system, the mRNA and activity of glutathione peroxidase (GPX) were greater (P<0.05) in the HS than in the CON group, whereas the HS+VC group showed markedly (P<0.05) lower GPX mRNA and activity than the HS group. However, superoxide dismutase, glutathione S-transferase, and malondialdehyde were unaffected by treatments. In conclusion, cyclic heat stress activated hepatic HSP70, TNF-α, iNOS, and GPX genes, whereas vitamin C during heat stress ameliorated heat stress-induced cellular responses in rats.

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Molecular cloning and characterization of chicken lipopolysaccharide-induced TNF-α factor (LITAF)

Cited by 119 publications

References 33 publications

In ovo and Dietary Supplementation of Probiotics Affects Post-Hatch Expression of Immune-Related Genes in Broiler Chicks

In ovo and Dietary Supplementation of Probiotics Affects Post-Hatch Expression of Immune-Related Genes in Broiler Chicks

Expression Profiles of Genes in Toll-Like Receptor-Mediated Signaling of Broilers Infected with Clostridium perfringens

Effects of Cyclic Heat Stress or Vitamin C Supplementation during Cyclic Heat Stress on HSP70, Inflammatory Cytokines, and the Antioxidant Defense System in Sprague Dawley Rats

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