Expression Profiles of Genes in Toll-Like Receptor-Mediated Signaling of Broilers Infected with
            <i>Clostridium perfringens</i>

Lü, Yang; Sarson, Aimie J.; Gong, Joshua; Zhou, Huaijun; Zhu, Weiyun; Kang, Zhumei; Yu, Hai; Sharif, Shayan; Han, Yanming

doi:10.1128/cvi.00254-09

Cited by 57 publications

(43 citation statements)

References 43 publications

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“…Therefore, the inclusion of MOS to broiler chickens might have a beneficial property on innate immune system function in the ileum of birds. The findings in the current study in the COC group are in agreement with the report from Lu et al (2009), where they showed no significant up/downregulation in TLR2 and TLR4 expressions in the ileum of birds challenged with C. perfringens 4 d postchallenge. However, there was a higher expression of TLR4 in both the ileum and cecal tonsil in MOSC treatment in the current study.…”

Section: Discussionsupporting

confidence: 92%

Innate immune response to yeast-derived carbohydrates in broiler chickens fed organic diets and challenged with Clostridium perfringens

et al. 2012

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Necrotic enteritis (NE) caused by Clostridium perfringens is a reemerging disease of economic importance in areas of the world where antibiotic growth promoters have been banned. The effect of mannan-oligosaccharide (MOS) supplementation in organic diets of broilers challenged with C. perfringens on performance, gut morphology, and innate immunity was investigated. Three hundred Ross-308 broilers were fed antibiotic-free certified organic starter and grower diets. On d 14, birds were orally challenged with 1 mL of C. perfringens culture at 3 × 10(10) cfu/bird. Treatments consisted of a control no-challenge (CO; 0 g/kg of MOS in the basal diet), control challenge (COC, 0 g/kg of MOS in the basal diet), and MOS challenge (2 g/kg of MOS in the basal diet). Challenge of birds resulted in decreased feed intake and BW gain (P = 0.048 and P = 0.026, respectively). Even though supplementation of diet with MOS improved feed intake (P = 0.985), BW gain and G:F were not improved compared with those of the CO group (P = 0.026 and P = <0.001, respectively). There was no significant difference among treatments in jejunal and ileal villus height, crypt depth, and goblet cells/mm(2) (P > 0.05). Quantitative real-time PCR showed that, in the ileum, the MOS diet resulted in an upregulation of toll-like receptor (TLR)2b, TLR4, interleukin (IL)-12p35, and interferon (IFN)-γ compared with CO (P = 0.003, P = 0.018, and P = 0.024, respectively). In the cecal tonsil, challenging birds with C. perfringens resulted in an upregulation of TLR2b compared with CO (P = 0.036), and MOS resulted in an upregulation of TLR4 (P = 0.018). In conclusion, feeding a MOS-supplemented diet to C. perfringens-challenged broiler chickens did not improve performance and gut morphology-associated responses. However, MOS was capable of altering TLR and cytokine profiles, where dual TLR2 and TLR4 pathways were associated with MOS supplementation with subsequent upregulation of ileal IL-12p35 and IFN-γ, implying that MOS supplementation in C. perfringens-challenged chickens supports a proinflammatory effect via T-helper cell-1 associated pathways.

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Section: Discussionsupporting

confidence: 92%

Innate immune response to yeast-derived carbohydrates in broiler chickens fed organic diets and challenged with Clostridium perfringens

et al. 2012

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“…Variation in immune response during the course of infection is attributed to the difference in disease susceptibility (Zekarias et al 2002). The TLR4 expression is up regulated in spleen of chickens challenged with Clostridium perfringens and S. enteritidis (Lu et al 2009, Abasht et al 2009). Moreover, TLR4 mRNA is differentially expressed in caecal tonsils of Salmonella-resistant and -susceptible lines of laying hens (Sadeyen et al 2006).…”

Section: Resultsmentioning

confidence: 99%

Toll-like receptor mRNA expression, iNOS gene polymorphism and serum nitric oxide levels in indigenous chickens

2011

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Toll-like receptor (TLR) family is one of the important members of innate immune system that recognizes conserved microbial patterns and induces innate immune response. They also act as a link to adaptive immune response. Nitric oxide (NO) is a multi-functional mediator with diverse physiological and immunological roles. In the present study TLR mRNA expression in heterophils, serum nitric oxide level and iNOS (inducible Nitric Oxide Synthase) gene polymorphism were investigated in cockerels of two Indian native chicken breeds, Aseel and Kadaknath. TLR (4 and 5) mRNA expression as quantified by real time RT-PCR revealed Kadaknath males expressed significantly (P < 0.01) higher TLR4 mRNA than Aseel males. iNOS gene polymorphism analyzed by PCR-RFLP method revealed difference in allele frequency. Kadaknath males had higher allele B frequency (0.81) than Aseel males (0.56). However, there were no genotype and breed effect on serum nitric oxide level. Based on the present study we conclude that Kadaknath has comparatively higher innate immunity levels than Aseel, however further investigations are needed.

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“…Given that Toll-like receptors (TLR) participate in pathogen detection and activate innate immune responses, Lu et al (2009) investigated the expression levels of chicken TLR after infection with C. perfringens. In summary, TLR1 type 1 levels in the spleen were upregulated at 1 d postinfection, whereas in the intestine, the levels were increased at d 2.…”

Section: Cytokine and Toll-like Receptor Expressionmentioning

confidence: 99%

Avian necrotic enteritis: Experimental models, host immunity, pathogenesis, risk factors, and vaccine development

et al. 2011

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The increasing trends of legislative restrictions and voluntary removal of antibiotic growth promoters worldwide has already affected, and will continue to affect, poultry production and animal health. Necrotic enteritis (NE) is being considered among the most important infectious diseases in the current poultry production system globally, with an estimated annual economic loss of more than $2 billion, largely attributable to medical treatments and impaired growth performance. Thus, there is an urgent need to develop rational, alternative, and integrated management strategies not only to control NE, but also to prevent it. In both humans and many warm-blooded animals and birds, NE is caused by Clostridium perfringens, a gram-positive, anaerobic, spore-forming bacterium. To accomplish these goals, better understanding of host- and environmentally related factors on the development of NE and potential vaccination strategies against C. perfringens infection will be necessary. Furthermore, a reliable and reproducible NE disease model is needed for characterization of C. perfringens pathogenesis and host protective immunity. This review summarizes recent developments in NE disease models, pathogenesis, host immunity, risk factors, and vaccine development for C. perfringens-associated NE in poultry.

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Expression Profiles of Genes in Toll-Like Receptor-Mediated Signaling of Broilers Infected with Clostridium perfringens

Cited by 57 publications

References 43 publications

Innate immune response to yeast-derived carbohydrates in broiler chickens fed organic diets and challenged with Clostridium perfringens

Innate immune response to yeast-derived carbohydrates in broiler chickens fed organic diets and challenged with Clostridium perfringens

Toll-like receptor mRNA expression, iNOS gene polymorphism and serum nitric oxide levels in indigenous chickens

Avian necrotic enteritis: Experimental models, host immunity, pathogenesis, risk factors, and vaccine development

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