Molecular cloning and characterization of a C-type lectin in roughskin sculpin (Trachidermus fasciatus)

Yu, Shanshan; Yang, Hui; Chai, Yingmei; Liu, Yingying; Zhang, Qiuxia; Ding, Xinbiao; Zhu, Qian

doi:10.1016/j.fsi.2012.11.033

Cited by 47 publications

(9 citation statements)

References 76 publications

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“…5, MnCTLDcp2 and MnCTLDcp3 was rapidly up-regulated to the highest level within 1 h and then decreased from 1 h, finally up-regulated at 36 he48 h which is similar to the expression pattern of EsCTLDcp from E. sinensis challenged with A. hydrophila [33]. Also, the expression of a C-type lectin TfCTL1 form Trachidermus fasciatus was stimulated by LPS challenge with a significant increase at 2 h post challenge, followed by three declines at 6 he24 h and rises at 48 h [38]. Based on our results and other studies, seem to suggest that the diverse nature of C-type lectins are involved in innate immunity against PAMPs and bacterial challenge in crustacean species.…”

Section: Discussionsupporting

confidence: 66%

“…An LDLa domain-containing C-type lectin EsCTLDcp [33] and three single-novel CRD containing C-type lectins TfCTL1, EsLecA and EsLecG [26,38] have been reported to agglutinate both Gram-positive and Gram-negative bacteria. In this research, MnCTLDcp2 and MnCTLDcp3 could also agglutinate two Grampositive bacteria (S. aureus and B. subtilis) and two Gramnegative bacteria (A. hydrophila and V. parahaemolyticus) (Fig.…”

Section: Discussionmentioning

confidence: 99%

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Isolation and characterization of two novel C-type lectins from the oriental river prawn, Macrobrachium nipponense

Xiu

Hou

Liu

et al. 2015

Fish & Shellfish Immunology

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of two novel C-type lectins from the oriental river prawn, Macrobrachium nipponense

Xiu

Hou

Liu

et al. 2015

Fish & Shellfish Immunology

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“…MaCTL transcripts were prominently presented in the loach liver, which is also true for many fish CTLs reported (Ji et al., 2014; Lv et al., 2016; Wang et al., 2017; Yu et al., 2013; Zhang, Hu, & Sun, 2010). Similarly, many crustacean CTLs are mainly distributed in their hepatopancreas, an equivalent of fish liver (Wang & Wang, 2013).…”

Section: Discussionsupporting

confidence: 65%

A C‐type lectin with antibacterial activity in weather loach, Misgurnus anguillicaudatus

Zhang

Yang

Zhang³

et al. 2020

Journal of Fish Diseases

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C‐type lectins are carbohydrate‐binding proteins that play important roles in immunity by serving as pattern recognition receptors. In the present study, a novel nattectin‐like C‐type lectin was obtained from the weather loach, Misgurnus anguillicaudatus, designated as MaCTL. MaCTL encodes a peptide with 165 amino acids, with a signal peptide and a single C‐type lectin domain (CTLD), containing a galactose‐specific QPD motif and a conserved Ca2+‐binding site. Transcripts of MaCTL were significantly upregulated after immune challenge with its pathogen A. hydrophila. In vitro assays with recombinant MaCTL protein revealed that it exhibited hemagglutinating and bacterial agglutinating activities, in a Ca2+‐dependent manner. MaCTL was found to bind to a wide range of bacteria, as well as bind to bacterial polysaccharides LPS and PGN. Moreover, MaCTL displayed antimicrobial activity by inhibiting the growth of bacteria. These results collectively suggest that MaCTL is involved in the antibacterial defence of weather loach.

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“…Ctl contained a QPD motif, indicating that it might be galactose specific, which was supported by the agglutination assay. The QPD motif has also been reported in C‐type lectin of T. fasciatus (Yu et al ., ). There is another type of EPD motif identified in fish lectins, e.g ., in Ec‐CTL in E. coioides (Wei et al ., ) and JFCTL in P. olivaceus (Kondo et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…Tissue distribution patterns of C-type lectins may show species differences. For example, SmLec1 in S. maximus, TfCTL1 in T. fasciatus and CsCTL1 in C. semilaevis have been reported largely expressed in liver (Zhang et al, 2010;Yu et al, 2013;Zhou & Sun, 2015). The expression of JFCTL in P. olivaceus was restricted to liver (Kondo et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and characterization of a C‐type lectin in yellow catfish Tachysurus fulvidraco

Fei

Zhang

Wang

et al. 2016

Journal of Fish Biology

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This study represents the first report of a C-type lectin (ctl) in yellow catfish Tachysurus fulvidraco. The complete sequence of ctl complementary (c)DNA consisted of 685 nucleotides. The open reading frame potentially encoded a protein of 177 amino acids with a calculated molecular mass of c.y 20.204 kDa. The deduced amino-acid sequence contained a signal peptide and a single carbohydrate recognition domain with four cysteine residues and GlnProAsp (QPD) and TrpAsnAsp (WND) motifs. Ctl showed the highest identity (56.0%) to the predicted lactose binding lectin from channel catfish Ictalurus punctatus. Quantitative real-time (qrt)-PCR analysis showed that ctl messenger (m)RNA was constitutively expressed in all examined tissues in normal fish, with high expression in trunk kidney and head kidney, which was increased following Aeromonas hydrophila challenge in a duration-dependent manner. Purified recombinant Ctl (rCtl) from Escherichia coli BL21 was able to bind and agglutinate Gram-positive and Gram-negative bacteria in a calcium-dependent manner. These results suggested that Ctl might be a C-type lectin of T. fulvidraco involved in innate immune responses as receptors (PRR).

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Molecular cloning and characterization of a C-type lectin in roughskin sculpin (Trachidermus fasciatus)

Cited by 47 publications

References 76 publications

Isolation and characterization of two novel C-type lectins from the oriental river prawn, Macrobrachium nipponense

Isolation and characterization of two novel C-type lectins from the oriental river prawn, Macrobrachium nipponense

A C‐type lectin with antibacterial activity in weather loach, Misgurnus anguillicaudatus

Molecular cloning and characterization of a C‐type lectin in yellow catfish Tachysurus fulvidraco

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