Isolation and characterization of two novel C-type lectins from the oriental river prawn, Macrobrachium nipponense

Xiu, Yunji; Hou, Libo; Liu, Xiaoqian; Wang, Yinghui; Gu, Wei; Meng, Qingguo; Wang, Wen

doi:10.1016/j.fsi.2015.07.011

Cited by 25 publications

(6 citation statements)

References 37 publications

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“…In the phylogenetic analysis of the shrimp CRDs (Fig. 2), MjGCTL clustered closely with four other CTLDs containing a QAP binding motif: LvCTLD25, MjLdlrLec 1 and 227, and MnCTLDcp 1 and 226. Among these, MjGCTL shares highest identity (77%) with LvCTLD (Supporting information, Fig.…”

Section: Discussionmentioning

confidence: 90%

“…Interestingly, all reported CTLDcps with QAP binding motifs are only found in shrimps, including MjGCTL, and they all contain a low-density lipoprotein receptor class A (LDLa) domain. Shrimp CTLDcps with an LDLa domain interact with viral particles2526 or respond to viral infection such as WSSV26. However, in the case of MjGCTL, mRNA levels remained unchanged after WSSV infection (Fig.…”

Section: Discussionmentioning

confidence: 94%

“…These inlcude CTLDs from Litopenaeus vannamei (LvCTLD)25 and Macrobrachium niponense (MnCTLDcp1,2)26 and LdlrLec1,2 from Marsupenaeus japonicus 27. The binding specificity of the QAP motif in LvCTLD, was originally reported to be Gal, suggesting QAP motif has the same specificity as QPD motif 25.…”

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confidence: 96%

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Pathogen recognition of a novel C-type lectin from Marsupenaeus japonicus reveals the divergent sugar-binding specificity of QAP motif

Alenton

Koiwai

Miyaguchi

et al. 2017

Sci Rep

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C-type lectins (CTLs) are calcium-dependent carbohydrate-binding proteins known to assist the innate immune system as pattern recognition receptors (PRRs). The binding specificity of CTLs lies in the motif of their carbohydrate recognition domain (CRD), the tripeptide motifs EPN and QPD bind to mannose and galactose, respectively. However, variants of these motifs were discovered including a QAP sequence reported in shrimp believed to have the same carbohydrate specificity as QPD. Here, we characterized a novel C-type lectin (MjGCTL) possessing a CRD with a QAP motif. The recombinant MjGCTL has a calcium-dependent agglutinating capability against both Gram-negative and Gram-positive bacteria, and its sugar specificity did not involve either mannose or galactose. In an encapsulation assay, agarose beads coated with rMjGCTL were immediately encapsulated from 0 h followed by melanization at 4 h post-incubation with hemocytes. These results confirm that MjGCTL functions as a classical CTL. The structure of QAP motif and carbohydrate-specificity of rMjGCTL was found to be different to both EPN and QPD, suggesting that QAP is a new motif. Furthermore, MjGCTL acts as a PRR binding to hemocytes to activate their adherent state and initiate encapsulation.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 94%

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Pathogen recognition of a novel C-type lectin from Marsupenaeus japonicus reveals the divergent sugar-binding specificity of QAP motif

Alenton

Koiwai

Miyaguchi

et al. 2017

Sci Rep

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“…C-type lectins are a family of calcium-dependent carbohydrate-binding proteins that are believed to play important roles in innate immunity in crustaceans [ 57 , 58 ]. In mammalian reproductive physiology, CTLs are involved in molecular mechanisms that underlie successful fertilization of embryos, a process via which sperm lectins recognize specific carbohydrates present on glycoproteins on the egg surface [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

Sex and tissue specific gene expression patterns identified following de novo transcriptomic analysis of the Norway lobster, Nephrops norvegicus

et al. 2017

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BackgroundThe Norway lobster, Nephrops norvegicus, is economically important in European fisheries and is a key organism in local marine ecosystems. Despite multi-faceted scientific interest in this species, our current knowledge of genetic resources in this species remains very limited. Here, we generated a reference de novo transcriptome for N. norvegicus from multiple tissues in both sexes. Bioinformatic analyses were conducted to detect transcripts that were expressed exclusively in either males or females. Patterns were validated via RT-PCR.ResultsSixteen N. norvegicus libraries were sequenced from immature and mature ovary, testis and vas deferens (including the masculinizing androgenic gland). In addition, eyestalk, brain, thoracic ganglia and hepatopancreas tissues were screened in males and both immature and mature females. RNA-Sequencing resulted in >600 million reads. De novo assembly that combined the current dataset with two previously published libraries from eyestalk tissue, yielded a reference transcriptome of 333,225 transcripts with an average size of 708 base pairs (bp), with an N50 of 1272 bp. Sex-specific transcripts were detected primarily in gonads followed by hepatopancreas, brain, thoracic ganglia, and eyestalk, respectively. Candidate transcripts that were expressed exclusively either in males or females were highlighted and the 10 most abundant ones were validated via RT-PCR. Among the most highly expressed genes were Serine threonine protein kinase in testis and Vitellogenin in female hepatopancreas. These results align closely with gene annotation results. Moreover, a differential expression heatmap showed that the majority of differentially expressed transcripts were identified in gonad and eyestalk tissues. Results indicate that sex-specific gene expression patterns in Norway lobster are controlled by differences in gene regulation pattern between males and females in somatic tissues.ConclusionsThe current study presents the first multi-tissue reference transcriptome for the Norway lobster that can be applied to future biological, wild restocking and fisheries studies. Sex-specific markers were mainly expressed in males implying that males may experience stronger selection than females. It is apparent that differential expression is due to sex-specific gene regulatory pathways that are present in somatic tissues and not from effects of genes located on heterogametic sex chromosomes. The N. norvegicus data provide a foundation for future gene-based reproductive studies.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3981-2) contains supplementary material, which is available to authorized users.

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“…142,143 Previous studies have shown that some immune-related genes such as C-type lectins (CTL) and heat shock protein 90 (HSP90) were also expressed and regulated in the hepatopancreas and gills of M. nipponense. 144,145 The fixed phagocytes in hepatopancreas can phagocytize and finally degrade the accumulated bacteria. 146 In addition, the hepatopancreas in crustaceans is an important source of molecules for humoral immunity, and these molecules include haemocyanin, various lectins, serine protease inhibitors and proteolytic enzymes.…”

Section: Otherorganswithimmune Regulationfunctionmentioning

confidence: 99%

Neuroendocrine‐immune regulation mechanism in crustaceans: A review

Tong

Pan

Zhang

et al. 2021

Reviews in Aquaculture

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With the increase of global population and seafood consumption, aquaculture is becoming more and more important for meeting global seafood demands. In the past decade, aquaculture production increased from 52.5 million tons in 2008 to 82.1 million tons in 2018. The estimated value of aquaculture production increased from USD 98.4 billion in 2008 to USD 250 billion in 2018. 1 Crustaceans contribute to 9.4 (11.4%) million tons of production, worth USD 69.3 billion in 2018. 1 However, disease problems have always been the restricting factor of crustacean culture, and has become the most significant hazard factor, causing substantial economic losses. The outbreak of crustacean diseases results from the invasion of external pathogens, the decline of animal immunity, and environmental stress. In particular, the deterioration of aquaculture environment can inhibit the immune system and increase the susceptibility of

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Isolation and characterization of two novel C-type lectins from the oriental river prawn, Macrobrachium nipponense

Cited by 25 publications

References 37 publications

Pathogen recognition of a novel C-type lectin from Marsupenaeus japonicus reveals the divergent sugar-binding specificity of QAP motif

Pathogen recognition of a novel C-type lectin from Marsupenaeus japonicus reveals the divergent sugar-binding specificity of QAP motif

Sex and tissue specific gene expression patterns identified following de novo transcriptomic analysis of the Norway lobster, Nephrops norvegicus

Neuroendocrine‐immune regulation mechanism in crustaceans: A review

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