Molecular Cloning and Characterization of Anti-Müllerian Hormone (AMH) from the Japanese Wrinkled Frog, Rana rugosa

Kodama, Masaya; Suda, Masashi; Sakamoto, Daisuke; Iwasaki, T; Matsuo, Yasuki; Uno, Yoshinobu; Matsuda, Yoichi; Nakamura, Yoriko; Maekawa, Shun; Katsu, Yoshinao; Nakamura, Masahisa

doi:10.1210/en.2013-2053

Cited by 18 publications

(11 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For all experiments, the ZZ/ZW type of R. rugosa was used. Unfertilized eggs were artificially ovulated and inseminated (Kodama et al., ). Fertilized eggs were developed to tadpoles 1 week after St. 25 and transferred into water containing 0, 0.002, 0.02, 0.2, 2.0, 2.7, 7.1, 18.8, or 50 ng/mL of T (ASKA Pharmaceutical Co., Ltd., Tokyo, Japan) to induce female‐to‐male sex reversal.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

A Threshold Dosage of Testosterone for Female‐to‐Male Sex Reversal in Rana rugosa Frogs

et al. 2016

Self Cite

View full text Add to dashboard Cite

Androgens play a critical role in testicular differentiation in many species of vertebrates. While female-to-male sex reversal can be induced by testosterone (T) in some species of amphibians, the mechanism still remains largely unknown even at the histological level. In this study, we determined a threshold dosage of T to induce female-to-male sex reversal in the Japanese frog Rana (R.) rugosa. Tadpoles were allowed to metamorphose into frogs with T present in the rearing water. At 0.2 ng/mL T, female frogs formed tissue comprising a mixture of ovary and testis, the so-called ovotestis, the size of which was significantly smaller than the wild-type ovary. Histological changes occurring in the oocytes of T-treated ovaries induced oocyte degeneration in the masculinizing ovaries leading to their final disappearance. In parallel, many germ cells emerged in the cortex of the ovotestis and, later, in the medulla as well. RT-PCR analysis revealed upregulated expression of CYP17 and Dmrt1 but not 17βHSD in the ovotestis, and downregulation of Pat1a expression. Furthermore, immunohistology revealed CYP17-positive signals in the cortex of the masculinizing ovary, spreading throughout the whole area as the testis developed. These results indicate that oocytes are sensitive to T in the ovary of R. rugosa and that male-type germ cells expand in the masculinizing gonad (testis) contemporaneous with oocyte disappearance.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Embryos and tadpoles were staged according to Shumway () and Taylor and Kollros (). The genetic sex of each tadpole was determined at the molecular level as reported elsewhere (Kodama et al., ).…”

Section: Methodsmentioning

confidence: 99%

A Threshold Dosage of Testosterone for Female‐to‐Male Sex Reversal in Rana rugosa Frogs

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, their evolution after the step of gonad differentiation is different from those observed in amniotes. The Wolffian ducts differentiate as uro-spermiducts in males (due to the absence of a mesonephros and secondary ureter in anamniotes) and [Kodama et al, 2015;Jansson et al, 2016]. The Amh protein has also been immunodetected in gonads of X. laevis , Bombina bombina , B. viridis , Hyla arborea , R. arvalis, and R. temporaria using a polyclonal antibody raised against the human protein [Piprek et al, 2013].…”

Section: Differentiation Of the Genital Tractmentioning

confidence: 99%

Sex Reversal in Amphibians

Flament¹

2016

Sex Dev

View full text Add to dashboard Cite

Amphibians have been widely used to study developmental biology due to the fact that embryo development takes place independently of the maternal organism and that observations and experimental approaches are easy. Some amphibians like Xenopus became model organisms in this field. In the first part of this article, the differentiation of the gonads in amphibians and the mechanisms governing this process are reviewed. In the second part, the state of the art about sex reversal, which can be induced by steroid hormones in general and by temperature in some species, is presented. Also information about pollutants found in the environment that could interfere with the development of the amphibian reproductive apparatus or with their reproductive physiology is given. Such compounds could play a part in the amphibian decline, since in the wild, many amphibians are endangered species.

show abstract

“…AMH is synthesised in the gonads of all vertebrate species examined to date, including fish (Western et al 1999, Halm et al 2007, amphibians (Kodama et al 2015), birds (Smith et al 1999, Nishikimi et al 2000, reptiles (Western et al 1999, Shoemaker et al 2007, marsupials (Juengel et al 2002, Pask et al 2004 and eutherian mammals (Josso 1973, Meyers-Wallen et al 1987, Kuroda et al 1990. In fish that do not have Müllerian ducts, AMH regulates the proliferation of germ cells in both sexes (Morinaga et al 2007).…”

Section: Amh Is a Gonadal Cytokinementioning

confidence: 99%

Anti-Müllerian hormone is a gonadal cytokine with two circulating forms and cryptic actions

McLennan¹,

Pankhurst²

2015

Journal of Endocrinology

View full text Add to dashboard Cite

Anti-Mü llerian hormone (AMH) is a multi-faceted gonadal cytokine. It is present in all vertebrates with its original function in phylogeny being as a regulator of germ cells in both sexes, and as a prime inducer of the male phenotype. Its ancient functions appear to be broadly conserved in mammals, but with this being obscured by its overt role in triggering the regression of the Mü llerian ducts in male embryos. Sertoli and ovarian follicular cells primarily release AMH as a prohormone (proAMH), which forms a stable complex (AMH N,C ) after cleavage by subtilisin/kexin-type proprotein convertases or serine proteinases. Circulating AMH is a mixture of proAMH and AMH N,C , suggesting that proAMH is activated within the gonads and putatively by its endocrine target-cells. The gonadal expression of the cleavage enzymes is subject to complex regulation, and the preliminary data suggest that this influences the relative proportions of proAMH and AMH N,C in the circulation. AMH shares an intracellular pathway with the bone morphogenetic protein (BMP) and growth differentiation factor (GDF) ligands. AMH is male specific during the initial stage of development, and theoretically should produce male biases throughout the body by adding a male-specific amplification of BMP/GDF signalling. Consistent with this, some of the male biases in neuron number and the non-sexual behaviours of mice are dependent on AMH. After puberty, circulating levels of AMH are similar in men and women. Putatively, the function of AMH in adulthood maybe to add a gonadal influence to BMP/GDF-regulated homeostasis.

show abstract

Molecular Cloning and Characterization of Anti-Müllerian Hormone (AMH) from the Japanese Wrinkled Frog, Rana rugosa

Cited by 18 publications

References 30 publications

A Threshold Dosage of Testosterone for Female‐to‐Male Sex Reversal in Rana rugosa Frogs

A Threshold Dosage of Testosterone for Female‐to‐Male Sex Reversal in Rana rugosa Frogs

Sex Reversal in Amphibians

Anti-Müllerian hormone is a gonadal cytokine with two circulating forms and cryptic actions

Contact Info

Product

Resources

About