Molecular cloning and characterization of two cDNAs encoding 1-deoxy-d-xylulose 5-phosphate reductoisomerase from Hevea brasiliensis

Seetang-Nun, Yortyot; Sharkey, Thomas D.; Suvachittanont, Wallie

doi:10.1016/j.jplph.2007.06.014

Cited by 37 publications

(14 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All classes of DXS isozymes have been found to participate in carotenoid biosynthesis. The rest of the MEP pathway enzymes are encoded by a single gene in most plants, with only some recently reported exceptions in the case of DXR and HDR Seetang-Nun et al, 2008;Kim et al, 2009b). Overexpression of DXS, DXR or HDR results in an increased production of carotenoids in light-grown Arabidopsis seedlings, whereas increased levels of other pathway enzymes such as HDS do not alter the levels of these plastidial isoprenoids (Estévez et al, 2001;Botella-Pavía et al, 2004;Carretero-Paulet et al, 2006;Flores-Perez et al, 2008a).…”

Section: Mep Pathway To Ggppmentioning

confidence: 99%

Carotenoid Biosynthesis in Arabidopsis: A Colorful Pathway

Ruiz-Sola

Rodríguez‐Concepción

2012

The Arabidopsis Book

452

346

View full text Add to dashboard Cite

Plant carotenoids are a family of pigments that participate in light harvesting and are essential for photoprotection against excess light. Furthermore, they act as precursors for the production of apocarotenoid hormones such as abscisic acid and strigolactones. In this review, we summarize the current knowledge on the genes and enzymes of the carotenoid biosynthetic pathway (which is now almost completely elucidated) and on the regulation of carotenoid biosynthesis at both transcriptional and post-transcriptional levels. We also discuss the relevance of Arabidopsis as a model system for the study of carotenogenesis and how metabolic engineering approaches in this plant have taught important lessons for carotenoid biotechnology.

show abstract

Section: Mep Pathway To Ggppmentioning

confidence: 99%

Carotenoid Biosynthesis in Arabidopsis: A Colorful Pathway

Ruiz-Sola

Rodríguez‐Concepción

2012

The Arabidopsis Book

452

346

View full text Add to dashboard Cite

show abstract

“…HDR, like DXS, is often encoded in plant genomes as multiple copies (Kim et al , 2008; Kim et al , 2009; Grassi et al , 2013; Kang et al , 2013), while the five intervening enzymes are encoded by singleton genes with few exceptions (Seetang-Nun et al , 2008). Gene duplications leading to multiple copies of a gene may arise through several unrelated mechanisms, including retroposition, unequal crossing over, segmental or chromosomal duplications, and polyploidy (Zhang, 2003; Shoemaker et al , 2006).…”

Section: Introductionmentioning

confidence: 99%

The 2-C-methylerythritol 4-phosphate pathway in melon is regulated by specialized isoforms for the first and last steps

Saladié

Wright

García-Más

et al. 2014

Journal of Experimental Botany

View full text Add to dashboard Cite

show abstract

“…Recently, a new twist was added to the picture. Hevea brasilensis was shown to harbor two copies of DXR gene, whose physiological signiWcance are not yet known (Seetang-Nun et al 2008), and the list of the multiple copy MEP pathway proteins is growing longer.…”

Section: Discussionmentioning

confidence: 99%

Two copies of 4-(cytidine 5′-diphospho)-2-C-methyl-d-erythritol kinase (CMK) gene in Ginkgo biloba: molecular cloning and functional characterization

Kim

Kuzuyama

et al. 2008

Planta

View full text Add to dashboard Cite

4-(Cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase (CMK or YchB), the fourth enzyme of the 2-C-methyl-D-erythritol 4-phosphate pathway, phosphorylates the 2-hydroxyl group of 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol in the presence of ATP. Two isogenes encoding CMK (GbCMK1 and GbCMK2) were cloned and characterized from Ginkgo biloba. The activities of both isozymes were confirmed by complementation assay using Escherichia coli NMW29, a ychB knock-out mutant. The transcript profiles of GbCMKs in the radicles and the cotyledons of the cultured Ginkgo biloba embryos demonstrated that the transcript levels of GbCMK1 were similar in both organs, whereas that of GbCMK2 was predominantly high in the ginkgolide-synthesizing radicles. Selective increases in the transcript abundance of GbCMK2 in the radicles, induced by light and methyl jasmonate treatments, were observed. These differential induction patterns of the transcripts imply GbCMK1 and GbCMK2 respectively have high correlations with the primary and the secondary metabolisms. The transit peptides of both isozymes delivered the fused green fluorescent protein (GFP) into the chloroplast in the Arabidopsis and the Nicotiana transient expression systems; interestingly, the transit peptide of GbCMK1 delivered the GFP protein into the cytosol and the nucleus in addition to the chloroplasts.

show abstract

Molecular cloning and characterization of two cDNAs encoding 1-deoxy-d-xylulose 5-phosphate reductoisomerase from Hevea brasiliensis

Cited by 37 publications

References 50 publications

Carotenoid Biosynthesis in Arabidopsis: A Colorful Pathway

Carotenoid Biosynthesis in Arabidopsis: A Colorful Pathway

The 2-C-methylerythritol 4-phosphate pathway in melon is regulated by specialized isoforms for the first and last steps

Two copies of 4-(cytidine 5′-diphospho)-2-C-methyl-d-erythritol kinase (CMK) gene in Ginkgo biloba: molecular cloning and functional characterization

Contact Info

Product

Resources

About