The 2-C-methylerythritol 4-phosphate pathway in melon is regulated by specialized isoforms for the first and last steps

Saladié, Montserrat; Wright, Louwrance P.; García-Más, Jordi; Rodríguez‐Concepción, Manuel; Phillips, Michael A.

doi:10.1093/jxb/eru275

Cited by 41 publications

(33 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DXS1 is a limiting factor in upregulation of the carotenoid pathway in A. thaliana 49 . DXS1 expression is induced by light 50,51 , and it is the main DXS isoform catalyzing the biosynthesis of isoprenoid and carotenoid precursors in photosynthetic metabolism 52,53 . DXS1 also regulates carotenoid accumulation in A. thaliana and tomato 54,55 .…”

Section: A Model For Carotenoid Accumulation In Carrot Rootsmentioning

confidence: 99%

A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution

et al. 2016

View full text Add to dashboard Cite

5 7Carrot (Daucus carota subsp. carota L.; 2n = 2x = 18) is a globally important root crop whose production has quadrupled between 1976 and 2013 (FAO Statistics; see URLs), outpacing the overall rate of increase in vegetable production and world population growth (FAO Statistics; see URLs) through development of high-value products for fresh consumption, juices, and natural pigments and cultivars adapted to warmer production regions 1 .The first documented colors for domesticated carrot root were yellow and purple in Central Asia approximately 1,100 years ago 2,3 , with orange carrots not reliably reported until the sixteenth century in Europe 4,5 . The popularity of orange carrots is fortuitous for modern consumers because the orange pigmentation results from high quantities of alpha-and beta-carotene, making carrots the richest source of provitamin A in the US diet 6 . Carrot breeding has substantially increased nutritional value, with a 50% average increase in carotene content in the United States as compared to 40 years ago 6 . Lycopene and lutein in red and yellow carrots, respectively, are also nutritionally important carotenoids, making carrot a model system to study storage root development and carotenoid accumulation.Carrot is the most important crop in the Apiaceae family, which includes numerous other vegetables, herbs, spices, and medicinal plants that enhance the epicurean experience 7 , including celery, parsnip, arracacha, parsley, fennel, coriander, and cumin. The Apiaceae family belongs to the euasterid II clade, which includes important crops such as lettuce and sunflower 8 . Genome sequences of euasterid I species have been reported, but only two genomes 9,10 have been published among the other euasterid II species.Here we report a high-quality genome assembly of a doubledhaploid orange carrot, characterization of the mechanism controlling carotenoid accumulation in storage roots, and the resequencing of 35 accessions spanning the genetic diversity of the Daucus genus. Our comprehensive genomic analyses provide insights into the evolution of the asterids and several gene families. These results will facilitate biological discovery and crop improvement in carrot and other crops.A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution We report a high-quality chromosome-scale assembly and analysis of the carrot (Daucus carota) genome, the first sequenced genome to include a comparative evolutionary analysis among members of the euasterid II clade. We characterized two new polyploidization events, both occurring after the divergence of carrot from members of the Asterales order, clarifying the evolutionary scenario before and after radiation of the two main asterid clades. Large- and small-scale lineage-specific duplications have contributed to the expansion of gene families, including those with roles in flowering time, defense response, flavor, and pigment accumulation. We identified a candidate gene, DCAR_032551, that conditions caro...

show abstract

Section: A Model For Carotenoid Accumulation In Carrot Rootsmentioning

confidence: 99%

A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Moreover, specialized isoforms of DXS can also regulate the MEP pathway in melons (Saladie et al . ). Previous studies have shown that the overexpression of DXS results in elevated levels of carotenoids and other terpenoids (Estévez et al .…”

Section: Discussionmentioning

confidence: 97%

“…As a major controlling enzyme of the MEP pathway, the activity of DXS can be regulated by different mechanisms (Banerjee & Sharkey 2014) such as feedback inhibition by IDP and DMADP (the final metabolites of the MEP pathway) (Banerjee et al 2013) and feedforward regulation by GAP (a substrate of DXS) (Patel et al 2012). Moreover, specialized isoforms of DXS can also regulate the MEP pathway in melons (Saladie et al 2014). Previous studies have shown that the overexpression of DXS results in elevated levels of carotenoids and other terpenoids (Estévez et al 2001;Enfissi et al 2005).…”

Section: Hc-pro Promotes Plastidic Isoprenoids Biosynthesis 2031mentioning

confidence: 99%

“…Our results revealed a possible mechanism of the MEP pathway affected by virus infection. As an intermediate in the MEP pathway, the levels of MEcDP (methylerythritol cyclodiphosphate) were also increased induced by DXS overexpression (Wright et al 2014). Recently, MEcDP was found to be a potential retrograde signalling molecule that regulates the expression of nuclearencoded, stress-responsive plastidial protein genes such as hydroperoxide lyase (HPL) (Xiao et al 2012).…”

Section: Hc-pro Promotes Plastidic Isoprenoids Biosynthesis 2031mentioning

confidence: 99%

“…Then, we identified an enzyme in the MEP pathway [1-deoxy-D-xylulose-5-phosphate synthase (NtDXS), or DXS] that could interact with PVY HC-Pro. DXS catalyses the rate-limiting step of the plastidic MEP pathway that produces isopentenyl diphosphate (Estévez et al 2001;Rohmer 2003;Xiang et al 2007;Banerjee et al 2013;Banerjee & Sharkey 2014;Saladie et al 2014;Wright et al 2014). Transgenic Arabidopsis thaliana with suppressed levels of DXS contained decreased amounts of isoprenoids such as chlorophylls, tocopherols, carotenoids, ABA and gibberellic acid (GA), while Arabidopsis overexpressing DXS had increased levels of these products (Estévez et al 2001).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Helper component‐proteinase enhances the activity of 1‐deoxy‐_D‐xylulose‐5‐phosphate synthase and promotes the biosynthesis of plastidic isoprenoids in Potato virus Y‐infected tobacco

Jin

et al. 2015

Plant Cell & Environment

View full text Add to dashboard Cite

Virus-infected plants show strong morphological and physiological alterations. Many physiological processes in chloroplast are affected, including the plastidic isoprenoid biosynthetic pathway [the 2C-methyl-D-erythritol-4-phosphate (MEP) pathway]; indeed, isoprenoid contents have been demonstrated to be altered in virus-infected plants. In this study, we found that the levels of photosynthetic pigments and abscisic acid (ABA) were altered in Potato virus Y (PVY)-infected tobacco. Using yeast two-hybrid assays, we demonstrated an interaction between virus protein PVY helper component-proteinase (HC-Pro) and tobacco chloroplast protein 1-deoxy-D-xylulose-5-phosphate synthase (NtDXS). This interaction was confirmed using bimolecular fluorescence complementation (BiFC) assays and pull-down assays. The Transket_pyr domain (residues 394-561) of NtDXS was required for interaction with HC-Pro, while the N-terminal region of HC-Pro (residues 1-97) was necessary for interaction with NtDXS. Using in vitro enzyme activity assays, PVY HC-Pro was found to promote the synthase activity of NtDXS. We observed increases in photosynthetic pigment contents and ABA levels in transgenic plants with HC-Pro accumulating in the chloroplasts. During virus infection, the enhancement of plastidic isoprenoid biosynthesis was attributed to the enhancement of DXS activity by HC-Pro. Our study reveals a new role of HC-Pro in the host plant metabolic system and will contribute to the study of host-virus relationships.

show abstract

Carrot Carotenoid Genetics and Genomics

et al. 2019

View full text Add to dashboard Cite

The 2-C-methylerythritol 4-phosphate pathway in melon is regulated by specialized isoforms for the first and last steps

Abstract: Highlight textThis research describes the role of specialized isoforms encoding regulated steps of the MEP pathway in melon and provides evidence for their functional specialization in seedling greening.

Cited by 41 publications

References 70 publications

A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution

A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution

Helper component‐proteinase enhances the activity of 1‐deoxy‐_D‐xylulose‐5‐phosphate synthase and promotes the biosynthesis of plastidic isoprenoids in Potato virus Y‐infected tobacco

Carrot Carotenoid Genetics and Genomics

Contact Info

Product

Resources

About

The 2-C-methylerythritol 4-phosphate pathway in melon is regulated by specialized isoforms for the first and last steps

Abstract: Highlight textThis research describes the role of specialized isoforms encoding regulated steps of the MEP pathway in melon and provides evidence for their functional specialization in seedling greening.

Cited by 41 publications

References 70 publications

A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution

A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution

Helper component‐proteinase enhances the activity of 1‐deoxy‐D‐xylulose‐5‐phosphate synthase and promotes the biosynthesis of plastidic isoprenoids in Potato virus Y‐infected tobacco

Carrot Carotenoid Genetics and Genomics

Contact Info

Product

Resources

About

Helper component‐proteinase enhances the activity of 1‐deoxy‐_D‐xylulose‐5‐phosphate synthase and promotes the biosynthesis of plastidic isoprenoids in Potato virus Y‐infected tobacco