Molecular Cloning and Characterization of &lt;small&gt;L&lt;/small&gt;-Galactose-1-phosphate Phosphatase from Tobacco (&lt;i&gt;Nicotiana tabacum&lt;/i&gt;)

Sakamoto, Shingo; Fujikawa, Yukichi; Tanaka, Nobukazu; Esaka, Muneharu

doi:10.1271/bbb.110995

Cited by 5 publications

(3 citation statements)

References 35 publications

(41 reference statements)

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“…L-Galactose-1-phosphate phosphatase (GPPase) is an AsA-biosynthetic enzyme participating in L-Gal production in the Lgalactose pathway, the principal AsA biosynthetic pathway in plants. 15) Hence GFP-conjugated Nicotiana tabacum GPPase (NtGPPase; accession no. EU700060) was transiently expressed in tomato fruit protoplasts.…”

Section: )mentioning

confidence: 99%

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Analysis of Ascorbic Acid Biosynthesis Using a Simple Transient Gene Expression System in Tomato Fruit Protoplasts

Sakamoto

Fujikawa

Esaka

2013

Bioscience, Biotechnology, and Biochemistry

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Section: )mentioning

confidence: 99%

“…The protein expressed in the transfected protoplasts was analyzed by the method described in the legend to Fig. 1, using anti-NtGPPase antiserum 15) (1:10,000 dilution with 4% skim milk). The black triangle shows immunoreactive NtGPPase protein band.…”

Section: )mentioning

confidence: 99%

Analysis of Ascorbic Acid Biosynthesis Using a Simple Transient Gene Expression System in Tomato Fruit Protoplasts

Sakamoto

Fujikawa

Esaka

2013

Bioscience, Biotechnology, and Biochemistry

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“…L-Galactose-1-P Phosphatase (GalPPase) catalyzes the synthesis of L-galactose (L-Gal), whose role as a key intermediate of AsA biosynthesis pathway has been demonstrated by strong biochemical and genetic evidence [11,12]. Thus this step is suggested probably more important in the formation of AsA.…”

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confidence: 99%

Metabolic engineering of vitamin C production in Arabidopsis

Xiao

Wang

et al. 2015

Biotechnol Bioproc E

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Vitamin C (L-ascorbic acid, AsA) is a compound which provides major nutritional value, both for plants and humans. In this study, three distinct metabolic engineering strategies, including overexpression of biosynthesis enzyme, suppression of catabolism enzyme and switching the sub-cellular localization of compartment enzyme, were employed to enhance the production of AsA in Arabidopsis thaliana. The results showed that (1) overexpression of L-Galactose-1-P Phosphatase (GalPPase) enhanced AsA content to 3.96 µmol/g FW, which was 1.6-fold more than that in their wild-type (WT) counterparts; (2) RNAi suppression of ascorbate oxidase (AO) resulted in a significant increase of AsA accumulation (0.86 µmol/g FW) in apoplast; (3) both mitochondrion-target and none-target overexpression of L-Galactose dehydrogenase (GalDH) did not significantly promote AsA production compared with WT (1.96 µmol/g), however a dramatic enhancement was observed following infiltration with L-galactono-1, 4-lactone (L-GalL), both in transgenic and WT plants. The best line produced AsA with the content of 3.90 µmol/g FW, which was about 2-fold of that in the untreated control (1.99 µmol/g FW). This study provides new strategies including GalPPase overexpression, AO suppression as well as L-GalL feeding for modern breeding aimed at stimulating the AsA content in plants.

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Chemistry and Metabolism of Ascorbic Acid in Plants

Yoshimura

Ishikawa²

2017

Ascorbic Acid in Plant Growth, Development and Stress Tolerance

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Molecular Cloning and Characterization of <small>L</small>-Galactose-1-phosphate Phosphatase from Tobacco (<i>Nicotiana tabacum</i>)

Cited by 5 publications

References 35 publications

Analysis of Ascorbic Acid Biosynthesis Using a Simple Transient Gene Expression System in Tomato Fruit Protoplasts

Analysis of Ascorbic Acid Biosynthesis Using a Simple Transient Gene Expression System in Tomato Fruit Protoplasts

Metabolic engineering of vitamin C production in Arabidopsis

Chemistry and Metabolism of Ascorbic Acid in Plants

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