Molecular cloning and characterization of the glutathione reductase gene from Stipa purpurea

Wang, Qiuli; Pu, Yanan; Yang, Danni; Yin, Xin; He, Zhao‐Rong; Yang, Yunqiang; Yang, Yongping

doi:10.1016/j.bbrc.2017.12.054

Cited by 15 publications

(12 citation statements)

References 45 publications

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“…In the presence of salt stress, yeast expressing genes from the salt-tolerant reed plants grew better than yeast expressing genes from salt-sensitive reed plants ( Takahashi, Liu & Takano, 2009 ; Takahashi et al, 2007 ). Recently, a GR gene named spGR was also cloned from Stipa purpurea into Arabidopsis thaliana , which subsequently allowed the genetically engineered plant to show greater tolerance to salt stress than the control plant ( Wang et al, 2018 ). Therefore, genes encoding GR from the common reed in our study can be the potential target genes in improving the salt tolerance of plants through genetic manipulation.…”

Section: Discussionmentioning

confidence: 99%

Differentially expressed genes related to oxidoreductase activity and glutathione metabolism underlying the adaptation of Phragmites australis from the salt marsh in the Yellow River Delta, China

Zhang

Chen

Feng

et al. 2020

PeerJ

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The common reed (Phragmites australis) is a dominant species in the coastal wetlands of the Chinese Yellow River Delta, where it tolerates a wide range of salinity. Recent environmental changes have led to the increase of soil salinity in this region, which has degraded much of the local vegetation. Clones of common reeds from the tidal marsh may have adapted to local high salinity habitat through selection on genes and metabolic pathways conferring salt tolerance. This study aims to reveal molecular mechanisms underlying salt tolerance in the tidal reed by comparing them to the salt-sensitive freshwater reed under salt stress. We employed comparative transcriptomics to reveal the differentially expressed genes (DEGs) between these two types of common reeds under different salinity conditions. The results showed that only three co-expressed genes were up-regulated and one co-expressed gene was down-regulated between the two reed types. On the other hand, 1,371 DEGs were exclusively up-regulated and 285 DEGs were exclusively down-regulated in the tidal reed compared to the control, while 115 DEGs were exclusively up-regulated and 118 DEGs were exclusively down-regulated in the freshwater reed compared to the control. From the pattern of enrichment of transcripts involved in salinity response, the tidal reed was more active and efficient in scavenging reactive oxygen species (ROS) than the freshwater reed, with the tidal reed showing significantly higher gene expression in oxidoreductase activity. Furthermore, when the reeds were exposed to salt stress, transcripts encoding glutathione metabolism were up-regulated in the tidal reed but not in the freshwater reed. DEGs related to encoding glutathione reductase (GR), glucose-6-phosphate 1-dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PD), glutathione S-transferase (GST) and L-ascorbate peroxidase (LAP) were revealed as especially highly differentially regulated and therefore represented candidate genes that could be cloned into plants to improve salt tolerance. Overall, more genes were up-regulated in the tidal reed than in the freshwater reed from the Yellow River Delta when under salt stress. The tidal reed efficiently resisted salt stress by up-regulating genes encoding for oxidoreductase activity and glutathione metabolism. We suggest that this type of common reed could be extremely useful in the ecological restoration of degraded, high salinity coastal wetlands in priority.

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Section: Discussionmentioning

confidence: 99%

Differentially expressed genes related to oxidoreductase activity and glutathione metabolism underlying the adaptation of Phragmites australis from the salt marsh in the Yellow River Delta, China

Zhang

Chen

Feng

et al. 2020

PeerJ

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“…It plays a critical role in the defense system against ROS, which occurs as a result of stress factors, by maintaining the GSH level and acts as a substrate for glutathione-Stransferases. In many recent studies, it was stated that GR-1 expression increased against cadmium stress in various plants [7,9,[31][32][33][34][35].…”

Section: Resultsmentioning

confidence: 99%

“…This also shows that; it is the ineffectiveness of the resistance mechanism against cadmium stress in both roots and shoots after the specified concentrations. It was determined that the GR pathway was inhibited due to possible cellular damage and it could not provide protection against cadmium stress by using the stress recovery pathway [7,[31][32][33][34].…”

Section: Resultsmentioning

confidence: 99%

“…Glutathione reductase, an enzyme especially active in the ascorbate-glutathione (ASH-GSH) pathway, plays a role in defense against ROS by maintaining the low status of GSH [30]. By determining the changes in gene expression levels of metallothionein and glutathione reductase in plants, the tolerance of plants to various types of stress can be determined [29][30][31][32][33].…”

Section: First Strand Cdna Synthesis Assaymentioning

confidence: 99%

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Expression analysis of Some Stress-Related Genes Induced by Cadmium on Tomato (Solanum lycopersicum L.) Plants

Bolukbasi

2021

Hittite Journal of Science and Engineering

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T omato is an important fruit crop grown mainly as an annual and economically valuable plant for growers in the Mediterranean basin (Solanum lycopersicum L. formerly Lycopersicon esculentum Mill. belongs to the Solanaceae family). It is an important source of vitamins, minerals, fiber and a dietary antioxidants [1]. It is also among the anti-carcinogenic foods due to the carotenoids it contains. Consumed as fresh and dried fruit, tomatoes are also processed in industry as tomato paste. Turkey, Egypt, Italy, Spain, Greece and Morocco are among the world's largest tomato producers and exporters [2].With the increasing population, unplanned urbanization and developing technology, heavy metal pollution has become an important environmental problem worldwide. In addition to these, as a result of industrial activities, mining, using pesticide in agriculture, metallurgy, combustion of fossil fuels, faulty waste disposal, metal-enriched some materials, automotive emissions and domestic wastes and many other factors [3][4][5]. He-Article History:

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“…Transgenic cotton overexpressing a mutated chloroplastic AtGR2 resulted in a 36-fold increase in GR activity, coupled with increased tolerance to short-term chilling stress (Kornyeyev et al, 2003; Logan et al, 2003). A recent report has shown that expressing a GR from Stipa purpurea in Arabidopsis resulted in greater tolerance to salt stress than that of wild-type (WT) plants (Wang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Tolerance to Methyl Viologen-Mediated Oxidative Stress via AtGR2 Expression From Chloroplast Genome

et al. 2019

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Owing to their sessile life habit, plants are continuously subjected to a broad range of environmental stresses. During periods of (a)biotic stresses, reactive oxygen species (ROS) levels can rise excessively, leading to oxidative stress. Glutathione reductase (GR) plays an important role in scavenging the ROS and maintenance of redox potential of the cell during oxidative stress. To enhance ROS scavenging capacity, and hence stress tolerance, the Arabidopsis thalianaGR2 (AtGR2) gene was expressed from the tobacco plastid (chloroplast) genome, the main source of ROS production in plant photosynthetic tissues, in this study. Leaves of transplastomic tobacco plants had about seven times GR activity and 1.5 times total glutathione levels compared to wild type. These transplastomic tobacco plants showed no discernible phenotype and exhibited more tolerance to methyl viologen-induced oxidative stress than wild-type control plants. The results indicate that introducing AtGR2 in chloroplasts is an efficient approach to increase stress tolerance. This study also provides evidence that increasing antioxidant enzyme via plastid genome engineering is an alternative to enhance plant’s tolerance to stressful conditions.

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Molecular cloning and characterization of the glutathione reductase gene from Stipa purpurea

Cited by 15 publications

References 45 publications

Differentially expressed genes related to oxidoreductase activity and glutathione metabolism underlying the adaptation of Phragmites australis from the salt marsh in the Yellow River Delta, China

Differentially expressed genes related to oxidoreductase activity and glutathione metabolism underlying the adaptation of Phragmites australis from the salt marsh in the Yellow River Delta, China

Expression analysis of Some Stress-Related Genes Induced by Cadmium on Tomato (Solanum lycopersicum L.) Plants

Enhanced Tolerance to Methyl Viologen-Mediated Oxidative Stress via AtGR2 Expression From Chloroplast Genome

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