Molecular characterization of zoonotic pathogens Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in calves in Algeria

Baroudi, Djamel; Khelef, Djamel; Hakem, Ahcène; Abdelaziz, Abdelhafidh; Chen, Xiaohua; Lysen, Colleen; Roellig, Dawn M.; Xiao, Lihua

doi:10.1016/j.vprsr.2017.02.005

Cited by 12 publications

(17 citation statements)

References 38 publications

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“… Country Positive/examined (%) Genotypes a ( n ) Ref. Algeria 11/102 (10.8) BEB4 (4), BEB6 (2), BEB3 (1), I (1), J (1), PtEb XI (1), mixed (1) [ 2 ] Argentina 10/70 (14.3) BEB4 (1); I (2), J (4); EbpC (1); BEB10 (1); D (1) [ 5 ] Australia 49/471 (10.4) I (18), J (14), BEB4 (6), TAR_fc2 (6), TAR_fc1 (1), TAR_fc3 (1) [ 48 ] Brazil 79/452 (17.5) I (35), BEB8 (23), BEB4 (7), BEB13 (7),BEB12 (5), D (4), BEB11 (3), EbpA (1), BEB14–BEB17 (1 each) [ 4 ] China 1817/10504 (17.3) J (904); I (519); BEB4 (151), BEB6 (31), O (27), CM8 (18), COS-I (14), EbpC (14), CHN3 (14), D (13), CHN1 (11), CGC3 (11), CGC2 (8), CHC8 (7), CGC1 (6), CHN4 (6), CS-4 (6), Type IV (5), CM19 (5), BEB10 (3), CHG2 (2), CHG3 (2), CHN–DC1 (2), CHN–DC2 (2), CHN–DC3 (2), G (2), NECA1–NECA5, CHC1–CHC7, CHC9–CHC17, N , BEB8, CD6, H , CC4, CSX1–CSX2, CHN15, CM21, PN, and mixed (1 each) [ 10 , 12 , 13 , 19 , 23 , 26 , 37 , ...…”

Section: Introductionmentioning

confidence: 99%

Genotyping and zoonotic potential of Enterocytozoon bieneusi in cattle farmed in Hainan Province, the southernmost region of China

Zheng¹,

Zhou²,

Ren³

et al. 2020

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Enterocytozoon bieneusi is an intestinal pathogen that infects a wide range of species, including humans. Cattle constitute an important host for E. bieneusi; however, there is a scarcity of information on the prevalence and genotyping of E. bieneusi in cattle in the Hainan Province of China. In this study, PCR analysis of 314 fecal samples from cattle in six cities of Hainan was performed for genotype identification. The average prevalence of E. bieneusi in these animals was 9.9% (31/314), and ranged from 0.0% (0/12) to 20.5% (8/39). Five known genotypes – EbpC (n = 14), BEB4 (n = 12), J (n = 2), I (n = 1), and CHG5 (n = 1) – and a novel genotype: HNC-I (n = 1) – were identified. Genotypes EbpC and HNC-I were placed in zoonotic Group 1, and the remaining four genotypes (BEB4, J, I, and CHG5) were placed in Group 2. Since 93.5% of the genotypes found in the cattle (29/31) (EbpC, BEB4, J, and I) have previously been found in humans, these genotypes are probably involved in the transmission of microsporidiosis to humans.

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Section: Introductionmentioning

confidence: 99%

Genotyping and zoonotic potential of Enterocytozoon bieneusi in cattle farmed in Hainan Province, the southernmost region of China

Zheng¹,

Zhou²,

Ren³

et al. 2020

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“…This was indeed the case of our study, where C. parvum was responsible for the majority (84%) of the infections in calves in the 2-30 days age group. Interestingly all C. parvum isolates were assigned to IIaA16G2R1, a sub-genotype less frequently documented causing bovine infections but already described in cattle in Algeria (Baroudi et al, 2017), Belgium (Geurden et al, 2007), and Spain (Quilez et al, 2008), and in yaks in China (Mi et al, 2013). Human infections by IIaA16G2R1 have also been reported in clinical and paediatric populations in Democratic Republic of São Tomé and Principe (Lobo et al, 2014) and Iran (Sharbatkhori et al, 2015;Ranjbar et al, 2016), highlighting the zoonotic potential of this C. parvum sub-genotype.…”

Section: Discussionmentioning

confidence: 97%

“…Human infections by IIaA16G2R1 have also been reported in clinical and paediatric populations in Democratic Republic of São Tomé and Principe (Lobo et al, 2014) and Iran (Sharbatkhori et al, 2015;Ranjbar et al, 2016), highlighting the zoonotic potential of this C. parvum sub-genotype. It is also worth mentioning that other members of the sub-type family IIa found infecting Algerian cattle include IIaA13G2R1 (Benhouda et al, 2017) and IIaA17G3R1 (Baroudi et al, 2017), whereas IIaA15G2R1 has not been identified in isolates of human or animal origin in the country to date.…”

Section: Discussionmentioning

confidence: 99%

“…Using the same diagnostic method, bovine cryptosporidiosis has been reported at infection rates varying from 19% to 84% in a limited number of epidemiological investigations conducted in the provinces of Bejaia, Setif, and Souk Ahras (Ouchene et al, 2014), Annaba and Eltarf (Hocine et al, 2016), and Batna (Benhouda et al, 2017) in the North-eastern part of the country. Molecular information on the presence and relative frequency of Cryptosporidium species are also scarce, with few studies reporting infections by C. bovis, C. ryanae, C. parvum, and C. andersoni in cattle (Baroudi et al, 2017;Benhouda et al, 2017), by C. baileyi and C. meleagridis in poultry and wild birds (Baroudi et al, 2013;Laatamna et al, 2017), and by C. erinacei, C. parvum, C. hominis, and C muris in horses and donkeys (Laatamna et al, 2013(Laatamna et al, , 2015. Although no molecular data from human isolates are currently available, taken together this information indicates that Cryptosporidium infections seem common in Algeria and transmission of the parasite may involve a number of different pathways.…”

Section: Introductionmentioning

confidence: 99%

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Cryptosporidium-associated diarrhoea in neonatal calves in Algeria

Ouakli

Belkhiri

Lucio

et al. 2018

Veterinary Parasitology: Regional Studies and Reports

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A B S T R A C TNeonatal calf diarrhoea triggered by the enteric protozoan parasite Cryptosporidium is a leading cause of morbidity and mortality in calves aged 1-month-old or younger globally. Infected cattle in general and calves in particular have also been demonstrated as major contributors of zoonotic C. parvum oocysts in the environment and have been linked to a number of waterborne outbreaks of human cryptosporidiosis. Little is known on the occurrence, geographical distribution, and molecular diversity of Cryptosporidium infections affecting bovine populations in Algeria. In this study faecal specimens were randomly collected from 460 cattle aged between two days and 18 months on 10 farms located in the provinces of Aïn Defla, Blida, Sétif, and Tizi Ouzou between the autumn of 2015 and the spring of 2016. Faecal samples were microscopically examined using the modified Ziehl-Neelsen acid-fast technique as screening method. Microscopy-positive samples were confirmed by a commercial coproantigen enzyme-linked immunosorbent assay (Bio-X Diagnostics). The identification of Cryptosporidium species and sub-genotypes in confirmed samples was conducted by PCR and sequence analyses of the small subunit ribosomal RNA (ssu rRNA) and the 60 kDa glycoprotein (gp60) genes of the parasite. Overall, 52.2% (240/460) of the investigated cattle tested positive to Cryptosporidium by microscopy. The infection was widespread in all 10 farms surveyed, but was significantly more prevalent in those from Blida in the central part of the country. Bovine cryptosporidiosis affected cattle of all age groups but with different outcomes. Pre-weaned (up to one month old) calves typically presented with diarrhoea, whereas older animals mostly harboured subclinical infections. The commercial ELISA used only detected 15.8% (38/240) of the samples that previously tested positive by microscopy, demonstrating a poor performance in field epidemiological surveys. Sequence analysis of the 29 isolates generated at the ssu rRNA loci confirmed the presence of four Cryptosporidium species including C. parvum (72.4%), C. bovis (13.8%), C. andersoni, (3.4%), and C. ryanae (3.4%). Two additional isolates (7.0%) could only be identified at the genus level. Eight out of the 21 isolates assigned to C. parvum were identified as sub-genotype IIaA16G2R1 at the gp60 locus. C. parvum was almost exclusively found infecting preweaned calves, whereas C. ryanae and C. andersoni were only detected in asymptomatic animals. Bovine cryptosporidiosis is highly endemic in the surveyed area and represents a veterinary public health concern that should be adequately tackled by Algerian veterinary health authorities and policy makers.

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“…Desde o primeiro relato mundial da criptosporidiose bovina em 1971 (Panciera et al, 1971), estudos epidemiológicos vem sendo realizados obtendo diferentes resultados quanto a infecção por parasitos do gênero Cryptosporidium nestes animais (Fayer et al, 2008;Meireles et al, 2011;Taylan-Ozkan et al, 2016;Baroudi et al, 2017;Matsuura et al, 2017;).…”

Section: Introductionunclassified

Criptosporidiose bovina: aspectos clínicos, epidemiológicos e terapêuticos

Teixeira

Vieira²,

Lopes

et al. 2019

Pubvet

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Resumo.A criptosporidiose bovina é causada principalmente pelas seguintes espécies do gênero Cryptosporidium: Cryptosporidium parvum, Cryptosporidium bovis, Cryptosporidium andersoni e Cryptosporidium rynae. O aparecimento de sinais clínicos em bovinos infectados está diretamente correlacionado a idade, estado imunitário do hospedeiro e da espécie do parasito responsável pela infecção. A diarreia é o sinal clínico mais evidente durante a criptosporidiose bovina, sendo normalmente observada em bezerros neonatos infectados por C. parvum. De todas as espécies do gênero Cryptosporidium que acomete bovinos, o C. parvum é a única que reconhecidamente apresenta potencial zoonótico. Os bovinos quando infectados por essa espécie, excretam grandes quantidades de oocistos do parasito no meio ambiente, fator muito importante na epidemiologia desta coccidiose. A ausência de drogas e vacinas eficazes contra a infecção por Cryptosporidium spp. em bovinos, apontam para a necessidade de medidas profiláticas voltadas ao manejo adequado dos animais. O objetivo desta revisão foi elucidar os aspectos clínicos, epidemiológicos e terapêuticos da criptosporidiose em bovinos.

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Molecular characterization of zoonotic pathogens Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in calves in Algeria

Cited by 12 publications

References 38 publications

Genotyping and zoonotic potential of Enterocytozoon bieneusi in cattle farmed in Hainan Province, the southernmost region of China

Genotyping and zoonotic potential of Enterocytozoon bieneusi in cattle farmed in Hainan Province, the southernmost region of China

Cryptosporidium-associated diarrhoea in neonatal calves in Algeria

Criptosporidiose bovina: aspectos clínicos, epidemiológicos e terapêuticos

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