Molecular characterization of VP7 gene of human rotaviruses from Bangladesh

Ahmed, Kawsar; Ahmed, Shohel; Mitui, Marcelo Takahiro; Rahman, Aminur; Kabir, Luthful; Hannan, Md. Abdul; Nishizono, Akira; Nakagomi, Osamu

doi:10.1007/s11262-010-0463-x

Cited by 22 publications

(22 citation statements)

References 56 publications

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“…The peak of rotavirus infection in Bhutanese children occurred at a young age, which is consistent with results found in other developing countries such as Bangladesh [18], but not in Turkey [19] or Sri Lanka [20]. By the age of 36–47 months, 100% of Bhutanese children have been infected with rotavirus, which is earlier than in other countries [16].…”

Section: Discussionsupporting

confidence: 86%

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A Prospective Hospital-based Surveillance to Estimate Rotavirus Disease Burden in Bhutanese Children under 5 Years of Age

et al. 2015

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As part of efforts to develop an informed policy for rotavirus vaccination, this prospective study was conducted to estimate the burden of rotavirus diarrhea among children less than 5 years old attended to the Department of Pediatrics, Jigme Dorji Wangchuk National Referral Hospital (JDWNRH), Thimphu, Bhutan. The duration of the study was three years, extending from February 2010 through December 2012. We estimated the frequency of hospitalization in the pediatric ward and dehydration treatment unit (DTU) for diarrhea and the number of events attributable to rotavirus infection among children under 5 years of age. During the study period, a total of 284 children (1 in 45) were hospitalized in the pediatric ward, and 2,220 (1 in 6) in the DTU with diarrhea among children residing in the Thimphu district. Group A rotavirus was detected in 32.5% and 18.8% of the stool samples from children hospitalized in the pediatric ward, respectively. Overall, 22.3% of the stool samples were rotavirus-positive, and the majority (90.8%) of them was detected in children under 2 years of age. From this study, we estimated that the annual incidence of hospitalization in the pediatric ward and DTU due to rotavirus diarrhea was 2.4/1000 (95% CI 1.7–3.4) and 10.8/1000 (95% CI 9.1–12.7) children, respectively. This study revealed that rotavirus is a major cause of diarrhea in Bhutanese children in Thimphu district and since no study has been performed previously, represents an important finding for policy discussions regarding the adoption of a rotavirus vaccine in Bhutan.

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Section: Discussionsupporting

confidence: 86%

“…The overall prevalence of rotavirus was similar to that reported from other South Asian countries, such as Sri Lanka (21.9%) [14] and India [15]. However, the prevalence was higher than that observed in Nepal (17%) [16] and Hong Kong (12%) [17] and lower than that in Bangladesh (39.5%) [18]. …”

Section: Discussionsupporting

confidence: 80%

A Prospective Hospital-based Surveillance to Estimate Rotavirus Disease Burden in Bhutanese Children under 5 Years of Age

et al. 2015

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“…16 The electropherotype was determined by polyacrylamide gel electrophoresis (PAGE) of genomic dsRNA. 17 Genomic dsRNA of rotavirus SA-11 was used as a reference to compare different patterns.…”

Section: Viral Genomic Rna Extraction Electropherotypes and Genotypesmentioning

confidence: 99%

Co-dominance of G1 and emerging G3 rotaviruses in Hong Kong: A three-year surveillance in three major hospitals

Mitui

Chan

Nelson

et al. 2011

Journal of Clinical Virology

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“…Although the prevalence of rotavirus diarrhea in Bangladesh and Turkey is similar [14,15], it is difficult to say why in Bangladeshi children the frequency of antigenemia was higher than Turkish children. Detection of rotavirus antigenemia is influenced by several factors such as increased acute-phase serum immunoglobulin G antibody titer [11], days after onset of diarrhea [11,12] and stool viral antigen levels [11].…”

Section: Discussionmentioning

confidence: 99%

“…This is part of studies on rotavirus diarrhea previously carried out in Turkey and Bangladesh [14,15]. Between September 2004 and December 2005, samples were collected from patients who attended at Gazi University Hospital and the Ministry of Health Ankara Educational and Research Hospital in Ankara, Turkey with watery diarrhea.…”

Section: Methodsmentioning

confidence: 99%

Circulating rotaviral RNA in children with rotavirus antigenemia

Ahmed

Bozdayı

Mitui

et al. 2013

J Negat Results BioMed

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BackgroundRotavirus antigenemia is a common phenomenon in children with rotavirus diarrhea, but information is scarce on aspects of this phenomenon, such as genotype specificity, presence of intact viruses and correlation between genomic RNA and antigen concentration. Such information may help in understanding rotavirus pathogenesis and eventually be useful for diagnosis, treatment and prevention.Methods and findingsSerum samples were collected from children who presented at hospitals with diarrhea. Antigenemia was present in 162/250 (64.8%) samples from children with rotavirus diarrhea. No specific rotavirus genotype was found to be associated with antigenemia. Rotavirus particles could not be found by electron microscopy in concentrated serum from children with high levels of antigenemia. In passaged rotavirus suspension a significant correlation (r = 0.9559; P = 0.0029) was found between antigen level and viral copy number, but no significant correlation (r = 0.001480; P = 0.9919) was found between antigenemia level and viral copy number in serum. When intact rotavirus was treated with benzonase endonuclease, genomic double-stranded (ds) RNA was not degraded, but when sera of patients with antigenemia were treated with benzonase endonuclease, genomic dsRNA was degraded, indicating genomic dsRNA was free in sera and not inside virus capsid protein.ConclusionsAntigenemia is present in a significant number of patients with rotavirus diarrhea. Rotavirus viremia was absent in the children with rotavirus diarrhea who participated in our study, and was not indicated by the presence of antigenemia. The significance of circulating rotavirus antigen and genomic dsRNA in serum of patients with diarrhea deserves further study.

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Molecular characterization of VP7 gene of human rotaviruses from Bangladesh

Cited by 22 publications

References 56 publications

A Prospective Hospital-based Surveillance to Estimate Rotavirus Disease Burden in Bhutanese Children under 5 Years of Age

A Prospective Hospital-based Surveillance to Estimate Rotavirus Disease Burden in Bhutanese Children under 5 Years of Age

Co-dominance of G1 and emerging G3 rotaviruses in Hong Kong: A three-year surveillance in three major hospitals

Circulating rotaviral RNA in children with rotavirus antigenemia

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