Co-dominance of G1 and emerging G3 rotaviruses in Hong Kong: A three-year surveillance in three major hospitals

Mitui, Marcelo Takahiro; Chan, Paul K.S.; Nelson, E. A. S.; Leung, Ting Fan; Nishizono, Akira; Ahmed, Kamruddin

doi:10.1016/j.jcv.2011.01.008

Cited by 28 publications

(19 citation statements)

References 38 publications

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“…The overall prevalence of rotavirus was similar to that reported from other South Asian countries, such as Sri Lanka (21.9%) [14] and India [15]. However, the prevalence was higher than that observed in Nepal (17%) [16] and Hong Kong (12%) [17] and lower than that in Bangladesh (39.5%) [18]. …”

Section: Discussionsupporting

confidence: 80%

“…Understanding the seasonal patterns of rotavirus has several implications, such as testing the efficacy of currently available vaccines and the vaccine implementation programs [25]. We found that rotavirus infection in Bhutan is predominantly seasonal, occurring in the winter-spring season, similar to the findings from countries in temperate climates [17, 26, 27]. Bhutan has different geographical regions, but as a whole, there are four seasons.…”

Section: Discussionsupporting

confidence: 53%

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A Prospective Hospital-based Surveillance to Estimate Rotavirus Disease Burden in Bhutanese Children under 5 Years of Age

et al. 2015

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As part of efforts to develop an informed policy for rotavirus vaccination, this prospective study was conducted to estimate the burden of rotavirus diarrhea among children less than 5 years old attended to the Department of Pediatrics, Jigme Dorji Wangchuk National Referral Hospital (JDWNRH), Thimphu, Bhutan. The duration of the study was three years, extending from February 2010 through December 2012. We estimated the frequency of hospitalization in the pediatric ward and dehydration treatment unit (DTU) for diarrhea and the number of events attributable to rotavirus infection among children under 5 years of age. During the study period, a total of 284 children (1 in 45) were hospitalized in the pediatric ward, and 2,220 (1 in 6) in the DTU with diarrhea among children residing in the Thimphu district. Group A rotavirus was detected in 32.5% and 18.8% of the stool samples from children hospitalized in the pediatric ward, respectively. Overall, 22.3% of the stool samples were rotavirus-positive, and the majority (90.8%) of them was detected in children under 2 years of age. From this study, we estimated that the annual incidence of hospitalization in the pediatric ward and DTU due to rotavirus diarrhea was 2.4/1000 (95% CI 1.7–3.4) and 10.8/1000 (95% CI 9.1–12.7) children, respectively. This study revealed that rotavirus is a major cause of diarrhea in Bhutanese children in Thimphu district and since no study has been performed previously, represents an important finding for policy discussions regarding the adoption of a rotavirus vaccine in Bhutan.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 53%

A Prospective Hospital-based Surveillance to Estimate Rotavirus Disease Burden in Bhutanese Children under 5 Years of Age

et al. 2015

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“…The VP6 and VP1 gene segments of the CAU12-2-51 strain clustered closely with strains originating from human/bovine-like rotavirus strains (BP1879, Hun5, KF17, PAH136, PAI11-96, Nov10-N507, and RUS-Nov06-K2), a feline/bovine-like G3P[9] strain (BA222), and a human/bovine-porcine like G12P[4] strain (L26; Figure 1c, 1d, and Table S2) [19], [33]–[37]. In contrast, VP2 , VP3 , and NSP2 gene segments were found to cluster together with most of the known rotavirus strains originating from human/bovine-like G6P[14] rotaviruses (B1711, Hun5, PAH136, PAI58, and KF17), a feline/bovine-like G3P[9] rotavirus (BA222), and bovine rotaviruses (NCDV, RF, WC3, 1063, and 1065; Figure 1e, 1f, 1h, and Table S2) [12], [19], [37], [39], [40], [42], [43], [44], [45], [46].…”

Section: Resultsmentioning

confidence: 99%

“…G3 rotavirus genotypes have a broad host range and they have been discovered in several host species. G3 has been shown to be the dominant genotype and has spread throughout many Asian countries, including Japan [43], China [44], Vietnam [45], and Hong Kong [46]. Recently, the incidences of the G2, G3, and G4 genotypes decreased, while the incidence of the G1 genotype increased, suggesting that the predominant genotypes of rotaviruses in South Korea can change rapidly within a short period of time [33].…”

Section: Discussionmentioning

confidence: 99%

Whole-Genome Analysis of a Rare Human Korean G3P[9] Rotavirus Strain Suggests a Complex Evolutionary Origin Potentially Involving Reassortment Events between Feline and Bovine Rotaviruses

et al. 2014

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A rare human rotavirus, G3P[9] strain RVA/Human-tc/KOR/CAU12-2-51/2013/G3P[9], was isolated from the stool of a 9-year-old female hospitalized with acute watery diarrhea in August 2012 in South Korea using a cell culture system, and its genome was analyzed. The complete genomic constellation of the CAU12-2-51 strain revealed a novel genotype constellation for human rotavirus, G3-P[9]-I2-R2-C2-M2-A3-N2-T3-E3-H3. Phylogenetic analysis revealed that the CAU12-2-51 strain originated from feline- and bovine-like reassortment strains. The genes encoding VP4, VP7, NSP1, NSP3, NSP4, and NSP5 were related to human/feline-like and feline rotavirus strains, whereas the remaining five genes encoding VP1, VP2, VP3, VP6, and NSP2 were related to the human/bovine-like and bovine rotavirus strains. This novel strain was identified for the first time, providing evidence of feline/bovine-to-human transmission of rotavirus. The data presented herein provide information regarding rotavirus diversity and evolution.

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“…An overall increase in G3 strains has recently being reported from many countries [14-16], some of them in association with the introduction of the pentavalent RVA vaccine [17,18]. How vaccine pressure has resulted in emergence of this genotype is unknown, but sustained and accurate monitoring is clearly desirable to clarify this.…”

Section: Resultsmentioning

confidence: 99%

Inaccurate identification of rotavirus genotype G9 as genotype G3 strains due to primer mismatch

Mitui

Chandrasena

Chan

et al. 2012

Virol J

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Reverse transcription (RT)-PCR is now the standard method for typing group A rotaviruses (RVA) to monitor the circulating genotypes in a population. Selection of primers that can accurately type the circulating genotypes is crucial in the context of vaccine introduction and correctly interpreting the impact of vaccination on strain distribution. To our knowledge this study is the first report from Asia of misidentification of genotype G9 as G3 due to a primer-template mismatch. We tested two published G-genotype specific primers sets, designed by Gouvea and colleagues (Set A) and Iturriza‐Gomara and colleagues (Set B) on RVA from Hong Kong and Sri Lanka. Among 52 rotaviruses typed as G3 by set A primers, 36 (69.2%) were identified as G9 by nucleotide sequencing and set B primers. Moreover, of 300 rotaviruses tested, 28.3% were untypable by set A primers whereas only 12.3% were untypable by set B primers. Our findings reinforce the need to periodically monitor the primers used for RVA genotyping.

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Co-dominance of G1 and emerging G3 rotaviruses in Hong Kong: A three-year surveillance in three major hospitals

Cited by 28 publications

References 38 publications

A Prospective Hospital-based Surveillance to Estimate Rotavirus Disease Burden in Bhutanese Children under 5 Years of Age

A Prospective Hospital-based Surveillance to Estimate Rotavirus Disease Burden in Bhutanese Children under 5 Years of Age

Whole-Genome Analysis of a Rare Human Korean G3P[9] Rotavirus Strain Suggests a Complex Evolutionary Origin Potentially Involving Reassortment Events between Feline and Bovine Rotaviruses

Inaccurate identification of rotavirus genotype G9 as genotype G3 strains due to primer mismatch

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