Molecular characterization of Toxoplasma gondii in pork meat from different production systems in the Czech Republic

Slaný, Michal; Reslová, Nikol; Babák, Vladimír; Lorencova, Alena

doi:10.1016/j.ijfoodmicro.2016.09.020

Cited by 35 publications

(25 citation statements)

References 32 publications

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“…Similar results were obtained in Spain (38.4%, MAT) [48], Italy (43.3%, ELISA) [32], Sweden (49.5%, ELISA) [49], and the Czech Republic (39.6%, ELISA) [9]. The percentage of seropositive wild boar was lower in several studies conducted in the Czech Republic (15.4% and 26.2%) [31,37], Spain (23.8%) [50] and Sweden (28.6%) [29], as well as in France (16.8% and 17.6%) [4,51], Switzerland (6.7%) [2], The Netherlands (24.4%) [39], Slovakia (8.1%) [3], Finland (33%) [52], Portugal (20.6%) [1], Denmark (27.7%) [53], Greece (5.2%) [25], Romania (16%) [54], Italy (14%) [55], Latvia (33.2%) [56] and Estonia (24%) [38].…”

Section: Discussionsupporting

confidence: 82%

“…In the current study, a commercial ELISA kit was used for detecting antibodies to T. gondii in serum and meat juice samples obtained from pigs which used an anti-pig IgG conjugate. Wild boar and pigs are close-related species belonging to the family Suidae, subfamily Sus [34], and tests using anti-pig IgG conjugate, intended for use in pigs only or in several animal species (pigs, ruminants, cats and dogs), have been successfully used in the diagnosis of T. gondii infections in wild boars [31,32,34,35,47,50,53,58]. Moreover, commercial ELISA kit intended for use in pigs, ruminants, cats and dogs, was used for comparison of the results obtained for wild boar by an in-house ELISA, and agreement between the two ELISA tests was evaluated as excellent [9].…”

Section: Discussionmentioning

confidence: 99%

“…Despite EFSA recommendations on the monitoring of T. gondii infections in sheep, goats, pigs and game animals [5], no such programme has been implemented for any food animals. Infection is usually asymptomatic and there is no way to distinguish infected from uninfected carcasses during slaughter inspection [5,31,34]. The tissue cysts of T. gondii are also undetectable during routine examination of meat for trichinosis, based on the magnetic stirrer method for pooled-sample digestion, referred to in Regulation 2015/1375 of 10 August 2015, which provides specific rules on official controls for Trichinella in meat.…”

Section: Discussionmentioning

confidence: 99%

“…The increase in their population size observed in recent years increases the risk of wild boar contact with people and pigs, and thus the risk of pathogen transmission, including zoonotic pathogens such as T. gondii [30]. The consumption of raw or undercooked meat is considered an important route of infection for humans [1,31,32,33,34]. However, the source of infection is difficult to determine [35], and varies depending on the populations tested (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence of <i>Toxoplasma gondii</i> antibodies in wild boar (Sus scrofa) from Strzałowo Forest Division, Warmia and Mazury Region, Poland

Puchalska¹,

Pyziel²,

Wisniewski³

et al. 2021

Ann Agric Environ Med.

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Introduction and objective. Toxoplasmosis is an important zoonosis caused by a protozoan, Toxoplasma gondii. Raw or undercooked venison may be a source of infection in humans. The aim of the study was to determine the prevalence of T. gondii antibodies in wild boar from the Strzałowo Forest Division of the Warmia and Mazury Region of Poland. Materials and method. A total of 90 samples were collected from 50 wild boar: 40 from both tongue and diaphragm muscles, 4 from diaphragm muscles and six from tongue muscles. Samples were analyzed using the commercial PrioCHECK® Toxoplasma Ab porcine ELISA, according to the manufacturer's instructions. Results. T. gondii antibodies were detected in 24 of 50 (48%) tested animals. T. gondii antibodies were detected in 40 of 90 (44.4%) tested samples (21 of tongue muscles and 19 of diaphragm muscles). In the 40 wild boar that provided samples of meat juice from the tongue and diaphragm muscles, specific antibodies were more prevalent in the tongue (20 of 40 animals-50%) than in the diaphragm muscles (17 of 40 animals-42.5%). Conclusions. The study revealed a high percentage of wild boar seropositive to T. gondii. Muscle samples to obtain meat juice are easily available and simple to collect, even on the hunting grounds, which makes them suitable material for detecting T. gondii antibodies in wild boar. Wild boar are essential to T. gondii circulation in the environment, and raw or undercooked venison may be a source of human infections with this parasite.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prevalence of <i>Toxoplasma gondii</i> antibodies in wild boar (Sus scrofa) from Strzałowo Forest Division, Warmia and Mazury Region, Poland

Puchalska¹,

Pyziel²,

Wisniewski³

et al. 2021

Ann Agric Environ Med.

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show abstract

“…Since tissue cysts are not visible on postmortem inspection, meat of infected animals represents a source of infection for man [34]. The risk of infection is further increased by the consumption of the hunted wild boar by hunters and their families, or the use of meat of infected animals in products intended for the local market.…”

Section: Introductionmentioning

confidence: 99%

The Seroprevalence of Toxoplasma gondii in Wild Boars from Three Voivodeships in Poland, MAT Analyses

et al. 2020

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Purpose The European wild boar (Sus scrofa) is a popular game animal species. Its meat, however, can represent a reservoir of dangerous foodborne diseases and can play an important role in the transmission of many pathogens, including Toxoplasma gondii, in humans and animals worldwide. The aim of the present study was to determine the presence of antibodies to T. gondii in the serum of hunted wild boars in Poland. Methods Using the commercial direct agglutination test, 398 serum samples collected during the hunting season 2009/2010 were tested for the presence of T. gondii antibodies, and the titre of 40 was considered indicative of T. gondii infection in analysed samples. Results It was found that nationwide, 37.7% were seropositive to T. gondii, although seroprevalence varied from 11.6 to 50% depending on the Voivodeship. Significant differences were observed between the Great Poland and Lubusz Voivodeships and between Great Poland and Warmian-Masurian. Conclusion Serological test indicated widespread exposure to T. gondii by wild boar; therefore, consumption of raw or undercooked game meat of infected animals can carry a significant risk of T. gondii infection.

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Detection of Toxoplasma gondii infection in buffaloes (Bubalus bubalis) and cattle (Bos taurus) at the Tabriz abattoir, Iran

Jabbari,

Hajipour,

Hassanzadeh

et al. 2024

Veterinary Medicine & Sci

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BackgroundToxoplasma gondii is a widely prevalent zoonotic protozoan parasite in humans and warm‐blooded animals worldwide. Infection of humans by this parasite can result in severe clinical symptoms, particularly in individuals with congenital toxoplasmosis or immunocompromised patients. Contamination mainly occurs through foodborne routes, especially the consumption of raw or undercooked meat from animals.ObjectivesThe aim of this study was to use PCR to detect T. gondii in tissues and organs of buffaloes and cattle slaughtered at Tabriz slaughterhouse, in Iran.MethodsFifty grams of heart, thigh, diaphragm and tongue from 50 buffaloes and 100 cattle slaughtered at the Tabriz industrial slaughterhouse were selected for sampling using a combination of convenience sampling. The samples were tested using a previously published PCR method.ResultsOut of the 150 animal samples, T. gondii was detected in 10 (6.7%, 95%CI: 3.2–11.9), including one buffalo (2%, 95%CI: 0.1–10.6) and nine cattle (9%, 95%CI: 4.2–16.4). There was no statistically significant difference in the rate of T. gondii infection among cattle based on age and sex (p > 0.05).ConclusionsThe results indicated a potential risk of T. gondii transmission to humans through the consumption of infected meat. Therefore, appropriate and effective preventive measures should be taken to limit the transmission of this parasite to humans, and the consumption of raw and undercooked meat should be discouraged.

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Molecular characterization of Toxoplasma gondii in pork meat from different production systems in the Czech Republic

Cited by 35 publications

References 32 publications

Prevalence of <i>Toxoplasma gondii</i> antibodies in wild boar (Sus scrofa) from Strzałowo Forest Division, Warmia and Mazury Region, Poland

Prevalence of <i>Toxoplasma gondii</i> antibodies in wild boar (Sus scrofa) from Strzałowo Forest Division, Warmia and Mazury Region, Poland

The Seroprevalence of Toxoplasma gondii in Wild Boars from Three Voivodeships in Poland, MAT Analyses

Detection of Toxoplasma gondii infection in buffaloes (Bubalus bubalis) and cattle (Bos taurus) at the Tabriz abattoir, Iran

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