Molecular Characterization of Tandem Repeat Protein 36 Gene of Ehrlichia canis Detected in Naturally Infected Dogs from Peru

Geiger, Joseph; Morton, Bridget A; Vasconcelos, Elton J R; Tngrian, Maryam; Kachani, Malika; Barron, Eduardo A.; Gavidia, César; Gilman, Robert H.; Angulo, Noelia; Lerner, Richard A.; Scott, Tamerin; Mir-Rashed, Nadereh; Oakley, Brian B.; Diniz, Pedro

doi:10.4269/ajtmh.17-0776

Cited by 13 publications

(12 citation statements)

References 42 publications

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“…Moreover, some dogs reacted only to TRP19, suggesting that unidentified E canis genotypes might be circulating in the region. TRP36 has been well characterized as a target for immune pressures elicited by the host, which leaves it susceptible to mutations 10,11,28 . This result was observed in a previous study, 12 and to date, a new genotype of E canis based on the TRP36 protein has been described in Costa Rica and Peru 13,28 …”

Section: Discussionsupporting

confidence: 68%

Canonical correlative analyses among an enzyme‐linked immunosorbent assay using synthetic peptides, an indirect fluorescent antibody test, and hematologic measurements in dogs infected with Ehrlichia canis

Taques

Koiyama

Campos

et al. 2020

Veterinary Clinical Pathol

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BackgroundImmunoreactive tandem repeat proteins (TRPs) in amino acid sequences were identified and employed in the serologic diagnosis of canine monocytic ehrlichiosis (CME).ObjectivesThis study evaluated using TRP19 and TRP36 synthetic protein antigens with enzyme‐linked immunosorbent assays (ELISAs) and compared the results with an indirect fluorescent antibody test (IFAT) to diagnose CME in the serum of dogs with suspected CME.MethodsThe sera of 243 dogs that exhibited clinical and hematologic signs suggestive of CME had IFATs performed. An ELISA with synthetic TRP19 and TRP36 antigens from two E canis genotypes (USTRP36 and BrTRP36) that circulate in Brazil were also performed. Canonical correlations and chi‐square tests were evaluated on the results of these tests.ResultsAmong the 243 dogs, 179 (73.6%) were reactive at an IFAT ≥ 40, 167 (68.72%) reacted at the cut‐off ≥160, and 149 (61.31%) reacted at ≥640. The ELISA revealed that 172 (70.8%) dogs reacted to TRP19 peptide, 163 (67.1%) reacted to USTRP36, and 114 (46.9%) reacted to BrTRP36. The canonical correlation analyses showed that the TRP19 ELISA was the most effective diagnostic method. Serum total protein levels showed strong positive correlations with the USTRP36 peptide. Qualitative analyses revealed an association between the TRP19 peptide and the presence of anemia, thrombocytopenia, and hyperproteinemia. The IFAT results at titers of ≥160 and ≥640 were associated with thrombocytopenia.ConclusionsThe results of this study indicated that the TRP19 peptide was an excellent antigen and that the IFAT was diagnostically important in confirming the diagnosis of CME in serum samples.

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Section: Discussionsupporting

confidence: 68%

Canonical correlative analyses among an enzyme‐linked immunosorbent assay using synthetic peptides, an indirect fluorescent antibody test, and hematologic measurements in dogs infected with Ehrlichia canis

Taques

Koiyama

Campos

et al. 2020

Veterinary Clinical Pathol

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“…The genotype CRTRP36 was first detected in the blood of human donors in Costa Rica, exhibiting a potential zoonotic relationship [ 9 ]. However, data from South American serosurveys reveal evidence of infection by this genotype only in dogs from Colombia and Peru [ 11 , 19 ].…”

Section: Discussionmentioning

confidence: 99%

Geographic Distribution of Ehrlichia canis TRP Genotypes in Brazil

Taques

Campos

Kavasaki

et al. 2020

Veterinary Sciences

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Tandem repeat proteins (TRPs) are major immunoreactive proteins of Ehrlichia canis, which have been used in the serological diagnosis of different genotypes of the microorganism. TRP19 is preserved among different E. canis isolates expressed on both reticulate and dense-core cells and observed in the extracellular matrix or associated with the morula membrane. TRP36 is differentially expressed only on the surface of the dense-core form of the bacterium and exhibits more divergence among isolates. The aim of this study was to evaluate the distribution of the American (USTRP36), Brazilian (BrTRP36) and Costa Rican (CRTRP36) genotypes of E. canis in Brazil, using ELISA assays. Serum samples of 814 dogs from 49 municipalities from all over Brazil were analyzed. Our results showed that 33.9% of the samples were reactive to the USTRP36 genotype and 32.6% to the BrTRP36 genotype. The two genotypes appeared to occur equally throughout Brazil, although the frequency of seropositivity was lower in the south than in the country’s other regions. Dogs that reacted to at least one of the synthetic peptides (TRP19 and TRP36) were 456 (56%). A few dogs (n = 5; 0.6%) reactive to the E. canis TRP36 genotype (CRTRP36) were also detected in the northeast and southern regions. We concluded that the American and Brazilian genotypes of E. canis are distributed evenly in Brazil, especially in the tropical region, while the temperate region in the south presented the lowest prevalence rates. This study offers the first report of dogs seropositive for the Costa Rican genotype in Brazil.

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“…These results contribute to determining the negative relationship between feral dogs and wild animals of REPSA regarding disease transmission. Ehrlichia canis (the causative agent of canine monocytic ehrlichiosis) is the main rickettsial agent registered in dogs of the Neotropical region, with prevalence ranging between 15 and 70% (BARRANTES-GONZÁLEZ et al, 2016;MONTENEGRO et al, 2017;ROTONDANO et al, 2017;GEIGER et al, 2018;PAULINO et al, 2018). Clinical signs of an E. canis infection can be variable, depending on the strain, the immune response of the dog, and the presence of concomitant infections with other tick or flea-borne pathogens (SAINZ et al, 2015), however, no clinical signs were observed in the animals captured.…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of Bartonella, Ehrlichia and Mycoplasma in feral dogs of El Pedregal de San Angel Ecological Reserve in Mexico City

Arenas

Gil-Alarcón

Sánchez‐Montes

et al. 2019

Rev. Bras. Parasitol. Vet.

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Free-ranging and feral dogs represent a group of unattended companion animals. They impact wild animal populations by predating native species, displacing predators and introducing exotic pathogens. The aim of this work was to describe the molecular occurrence of Rickettsia, Ehrlichia, Anaplasma, Mycoplasma and Bartonella in feral dogs. The study was carried out in the last relict of a protected area in Mexico City. Blood clots samples from 19 dogs were obtained and analyzed for detection of specific fragments of the 16S-rRNA gene for Anaplasma, Ehrlichia and Mycoplasma and citrate synthase (gltA) for Bartonella and Rickettsia. Our results showed that DNA from three bacteria species (Bartonella vinsonii subsp. berkhoffii, Ehrlichia canis and Mycoplasma haemocanis) was present with frequencies ranging from 5.3 to 15.8%. This is the first record of B. vinsonii subsp. berkhoffii and M. haemocanis in dogs from México, and also the first finding of Ehrlichia canis in Mexico City. It is important to perform surveillance of feral dog populations in order to identify the impact of these pathogens on wild animal populations and Public Health in order to establish prevention and protection programs.

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Molecular Characterization of Tandem Repeat Protein 36 Gene of Ehrlichia canis Detected in Naturally Infected Dogs from Peru

Cited by 13 publications

References 42 publications

Canonical correlative analyses among an enzyme‐linked immunosorbent assay using synthetic peptides, an indirect fluorescent antibody test, and hematologic measurements in dogs infected with Ehrlichia canis

Canonical correlative analyses among an enzyme‐linked immunosorbent assay using synthetic peptides, an indirect fluorescent antibody test, and hematologic measurements in dogs infected with Ehrlichia canis

Geographic Distribution of Ehrlichia canis TRP Genotypes in Brazil

Molecular detection of Bartonella, Ehrlichia and Mycoplasma in feral dogs of El Pedregal de San Angel Ecological Reserve in Mexico City

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