Molecular Characterization of Some Virulence Genes of Salmonella enterica Serotype Sandow and Saintpaul Isolated from Environment of Dairy Farms at Assiut Province, Egypt

Sedeek, Eman K.; Sedeek, Sotohy Ahmed Sotohy Eman Khalifa; Sotohy, Sotohy Ahmed

doi:10.4172/2157-7579.1000559

Cited by 1 publication

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“…According to (Table 4), the considerable differences in virulence determinants of Salmonella serovars in this study may be attributed to the variation in sample sources, types of serovars and presence or absence of plasmids carrying virulence associated genes, this is in accordance with (Porwollik et al, 2004) and this is could be showed by (Sotohy et al, 2018), who collected (95) samples from three dairy farms at 3 different localities in Assiut Province including for detection of Salmonella species by molecular characterization for the presence of 6 virulence genes; pefA (700 bp), mgtC (677 bp), stn (617 bp), sopB (517 bp), invA (284 bp) and avrA (422 bp) in Salmonella isolates revealed that all 6 tested virulence genes were detected in Salmonella enterica serotype isolated from manure.…”

Section: Discussionsupporting

confidence: 59%

Characterization of Salmonella spp. Isolated From Poultry Giblets, Calves and Human Beings in Menoufiya Governorate

Elwaraqi

Bayomi

Zidan

2019

Journal of Current Veterinary Research

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Salmonellosis remains as an important public health problem and of economic importance worldwide. The aim of this study was to indicate the epidemiology of salmonellosis in both animal and human being in Menofiya Governorate. A total of 352 samples from poultry giblets (52 liver, 32 gizzard, 30 heart), frozen minced meat (58), calves faeces (50) and 130 stool samples were collected and examined bacteriologically, biochemically. Our results revealed that the prevalence of Salmonella spp. was 71.15% (37/52), 59.4% (19/32), 60% (18/30), 24.14% (14/58), 6% (3/50) and 42.3% (55/130) in liver, gizzard, heart, frozen minced meat, calves fecal matter and human stool, respectively. Moreover, 10 isolates of them had been serotyped and for further confirmation 33 isolates from these sources were examined using PCR to detect presence of Salmonella species invA gene and virulence genes (mgtC and hilA). By serotyping S.typhimurium was detected in liver, heart, frozen minced meat and calves fecal matter, S.enteritidis was found in gizzard and human stool samples, while S.kentucky was only detected from human stool samples. Furthermore, PCR on 33 isolates detected (100%) for invA, (84.85%) for mgtC and (90.9%) for hilA gene. In conclusion, Salmonella species has potential zoonotic spread and the presence of virulence genes in isolates from animals, products and in-contact human being, confirming the role of food from animal origin in transmission of salmonellosis.

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Section: Discussionsupporting

confidence: 59%