Molecular Characterization of Sarcocytis Fusiformis and Sarcocystis Buffalonis Infecting Water Buffaloes (Bubalus Bubalis) From Egypt

Abstract

Parasites of the genus Sarcocystis have the ability to infect wide range of animals, birds and reptiles. Water buffaloes are intermediate hosts for four Sarcocystis species; S. fusiformis, S. buffalonis, S. levinei and S. dubeyi. There was no previous molecular identification of Sarcocystis spp. parasitizing water buffaloes from Egypt. The current study was established to screen infection rates and apply genetic identification of the macroscopic Sarcocystis spp. infecting Egyptian water buffalos in El-Gharbia province, Egypt. Sarcocysts of both S. fusiformis and S. buffalonis were grossly examined through meat inspection in slaughterhouse, followed by histological identification of the detected sarcocysts. Finally, molecular identification using PCR, sequencing and phylogenetic analysis of the 18S rRNA gene was conducted. Comparison of the obtained sequences of the local tested isolates of both S. fusiformis and S. buffalonis with those previously reported for different Sarcocystis species those were recorded in Genbank, revealed that all the tested specimens represented both S. fusiformis and S. buffalonis. Moreover, sequence alignments of the partial 18S rRNA gene ascertained the presence of genetic variations revealing the heterogenic nature of the gene among different strains of the same Sarcocystis species in relation to the geographic distribution.

Detection of Humoral and Cellular Immune Responses in Buffaloes Naturally Infected with Sarcocystosis with Risk Factor Assessment

“…The cyst wall was smooth, thick and measured about 1.5 -4 µm. The obtained result is supported by observation of El-Dakhly et al [9], El-Seifyet al [33] where they found that S.fusiformis cyst wall measured 1-3 µm and 2.6 -14.5 µm respectively.…”

Molecular approaches in species identification of Sarcocysts in Indian buffalo meat

“…1 and 2). Similar observations were made by El -Seify et al 2014. S. fusiformis infecting water buffaloes was studied in different countries and showed high incidences like 83.3% in Turkey (Retzlaff and Weise, 1969), 94% in China (Xiao et al, 1988) and 88% in Vietnam (Huong et al, 1995).…”

DETECÇÃO ATRAVÉS DE REAÇÃO EM CADEIA PELA POLIMERASE EM TEMPO REAL SEGUIDA DE ANÁLISE DE ALTA RESOLUÇÃO DE CURVAS DE DISSOCIAÇÃO (qPCR-HRM) DE PROTOZOÁRIOS PARASITOS (APICOMPLEXA: SARCOCYSTIDAE) EM AMOSTRAS DE EMBUTIDOS DE ORIGEM SUÍNA

“…Das amostras analisadas em Yazd, Irã (Hajimohammadi et al, 2014), 77,9% foram positivas para Sarcocystis spp. No Egito do total de amostras analisadas, 53% e 12,3% estavam infectadas por S. fusiformis e S. buffalonis, respectivamente (El-Seify, 2014). No norte da Índia pela PCR convencional 72,8% das amostras foram positivas, enquanto pela qPCR houve positividade em 76,4% (Kaur et al, 2015).…”