Molecular characterization of Salmonella isolates by REP-PCR and RAPD analysis

Albufera, U.; Bhugaloo-Vial, P.; Issack, Mohammad Iqbal; Jaufeerally‐Fakim, Yasmina

doi:10.1016/j.meegid.2007.12.003

Cited by 50 publications

(38 citation statements)

References 35 publications

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“…Genetic diversity of Salmonella serovars was described in a number of studies, and molecular methods such as random amplified polymorphic DNA (RAPD), repetetive sequencebased PCR (REP-PCR), enterobacterial repetitive intergenic consensus sequence-based PCR (ERIC-PCR), and PFGE have been implied for characterization of the Salmonella strains (Shabarinath et al 2007;Ponce et al 2008;Albufera et al 2009). Identical or closely related Salmonella types from different foods including meat, poultry, and fish were observed, and despite the fact that data focusing on Salmonella genetic diversity with linkage to human salmoneliosis is limited, fish and fish products remain as important sources of Salmonella infection.…”

Section: Microflora Of Fish and Fish Environmentsmentioning

confidence: 99%

Major foodborne pathogens in fish and fish products: a review

et al. 2015

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Fish plays an important role in the human diet, and there is an observed increase in the consumption of fish per capita in Europe. However, intensive growth of industry and agriculture may cause contamination of natural and humanmade aquatic environments, and may affect not only the health of fish, but also raise safety concerns with regard to fish used for human consumption. It is well known that fish and fish products are often associated with human diseases. Thus, it is necessary to study the prevalence of pathogens in fish to ensure the safety of fish products and environments. Microbial assessment of fish also gives additional information about the hygienic status of environments, including lakes, rivers, ponds, and fish farms. Detection of pathogenic microorganisms or changes in natural microflora in the water environment could be an important indicator of possible contamination. The aim of this review was to describe and discuss the five most relevant bacterial genera and species linked to aquatic environments-Vibrio spp., Listeria monocytogenes, Yersinia spp., pathogenic Salmonella serovars, and Clostridium botulinum-causing human foodborne diseases.

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Section: Microflora Of Fish and Fish Environmentsmentioning

confidence: 99%

Major foodborne pathogens in fish and fish products: a review

et al. 2015

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“…Salmonella Typhi causes enteric fever in humans whereas Salmonella Typhimurium causes systemic disease in mice which closely resembles typhoid fever in humans (Lin et al, 2007). In the United States, approximately 40,000 cases of salmonellosis are reported each year which cause about 600 deaths annually (Albufera, Bhugaloo-Vial, Issack, & Jaufeerally-Fakim, 2009). In Malaysia, it is difficult to evaluate the status of salmonellosis due to the lack of detailed epidemiological studies by the public health and veterinary sector.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR

et al. 2011

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a b s t r a c tThis study aimed to determine the prevalence and quantity of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits from hawker stalls and hypermarkets in Malaysia. Analysis was carried out using the most probable number (MPN) e multiplex polymerase chain reaction (PCR) method. The prevalence of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in 210 samples of sliced fruits examined were 23.3%, 7.6% and 3.8%, respectively with estimated quantity varying from 0 to 19 MPN/g. This study urged the authority to look into the biosafety of sliced fruits in Malaysia.

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“…Diseases in both animals and humans worldwide are caused by different serotypes and strains of the bacterial species Salmonella enterica subsp. enterica (Albufera et al, 2009;Pan et al, 2010). Through the ages, wildlife has been a sig-nificant source of infectious diseases transmissible to humans (Mirzaie et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Prevalence and genetic characteristics of Salmonella strains in wild Mallard ducks (Anas platyrhynchos) in Semnan suburb, Iran

Staji¹,

Rezaei²,

Rassouli³

et al. 2017

BJVM

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Wild birds serve as major reservoirs for transmission of Salmonella to domestic animals and humans. Given the zoonotic potential of salmonellosis, the main goal of this research was to investigate the prevalence and molecular epidemiology of S. enterica infections in wild Mallard ducks. Faecal samples (n=247) from wild Mallard ducks were tested for the prevalence of Salmonella spp., and genotypes of strains were then differentiated by multiplex PCR. From the 247 faecal samples, 18 (7.29%) were positive for Salmonella spp. Biochemically the most predominant serovars were S. Typhimurium and S. Enteritidis (10 and 6 cases each, respectively), whereas only 2 serovars belonged to S. Infantis. Among the 10 S. Typhimurium serovars, nine strains were positive for rfbJ, fljB, invA, and fliC genes based on multiplex PCR assay and one strain contained only the invA gene. In S. Enteritidis serovars, PCR generated amplification products for spv and sefA genes, and a random sequence in all samples. The two S. Infantis contained the random sequence specific for Salmonella genus. With respect to the circulation of virulent Salmonella in wild ducks of Semnan suburbs, more work to assess the correlation of strains from wild life with human and livestock strains is needed.

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Molecular characterization of Salmonella isolates by REP-PCR and RAPD analysis

Cited by 50 publications

References 35 publications

Major foodborne pathogens in fish and fish products: a review

Major foodborne pathogens in fish and fish products: a review

Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR

Prevalence and genetic characteristics of Salmonella strains in wild Mallard ducks (Anas platyrhynchos) in Semnan suburb, Iran

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